ID A0A170YUD1_9ACTN Unreviewed; 1183 AA.
AC A0A170YUD1;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=STXM2123_3421 {ECO:0000313|EMBL:GAT82720.1};
OS Streptomyces sp. F-3.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1840095 {ECO:0000313|EMBL:GAT82720.1, ECO:0000313|Proteomes:UP000078145};
RN [1] {ECO:0000313|EMBL:GAT82720.1, ECO:0000313|Proteomes:UP000078145}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Wang L.S., Gao P.J., Liu L., Zhang H.Q., Cheng Z., Sun X.M.;
RT "Streptomyces sp. F-3 geneom sequencing.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAT82720.1}.
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DR EMBL; BDDR01000020; GAT82720.1; -; Genomic_DNA.
DR RefSeq; WP_067396476.1; NZ_BDDR01000020.1.
DR AlphaFoldDB; A0A170YUD1; -.
DR STRING; 1840095.STXM2123_3421; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000078145; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000078145}.
FT DOMAIN 647..808
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 826..983
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1183 AA; 129482 MW; C3C24D2BF0AD50BE CRC64;
MTLHGLLDAV VKDPALAEAI TAAADGNRMH IDLVGPPAAR PLAVAALARE TARPVLAVTA
TGREAEDLAD ALRSLLPPHG VVEYPAWETL PHERLSPRSD TMGRRLAVLR RLAHPDPDDP
ETGPVSVVVA PVRSVLQPQV KGLGDLEPVS LRTGQSADLD DVVEALAAAA YSRVELVEKR
GEFAVRGGIL DVFPPTEEHP LRIEFWGDDV EEIRYFKVAD QRSLEVAEHG LWAPPCRELL
LTEDVRARAR ELAERHPELG ELLGRIAEGI AVEGMESLAP VLVDDMELLL DVLPEGAMTV
VCDPERVRTR AADLVATSQE FLQASWAATA GGAEAPIDVG AASLRSIADI RDHARELGMM
WWTVSPFAAD ETAYGGEDEG ADTLKLGMRA PETYRGDTAR ALADTKGWLA DGWRTVFVTE
GHGPASRTVE VLGGEGIAAR LDVDLKELSP SVVHVACGSV THGFVDPGLK LAVLTETDLT
GQKTAGRDGA RMPVRRRKSI DPLTLEPGDY IVHEQHGVGR YIEMVQRTVQ GATREYLVVE
YAPAKRGQPG DRLYIPTDQL EQITKYVGGE APTLHRLGGA DWTKTKARAR KAVKEIAADL
IKLYSARMAA PGHAFGPDTP WQRELEDAFP YTETPDQLTT IAEVKADMEK PVPMDRLICG
DVGYGKTEIA VRAAFKAVQD GKQVAVLVPT TLLVQQHFAT FSERYAQFPV NVKALSRFQT
DAEAKAVLDG LRDGSVDVVI GTHRLFSSET KFKDLGLVIV DEEQRFGVEH KEQLKKLRAN
VDVLTMSATP IPRTLEMAVT GIREMSTITT PPEERHPVLT FVGPYDEKQI GAAIRRELLR
EGQVFYIHNR VESIDRAAAR LREIVPEARI ATAHGQMSEA ALEQVVVDFW EKKYDVLVST
TIVESGIDIS NANTLIVERS DTFGLSQLHQ LRGRVGRGRE RGYAYFLYPP EKPLTETAHE
RLATIAQHTE MGAGMYVAMK DLEIRGAGNL LGGEQSGHIA GVGFDLYVRM VGEAVADYRR
QLETGEIKEE PPLEVKIELP VDAHVPHDYA PGERLRLQAY RSIASANSEE DIKAVREELT
DRYGKLPEPV ENLLLVAGLR MLARACGVSE IVLQGTNIRF APVELRESQE LRLKRLYPGS
VIKPAVHQVL VPRPKTAKVG GKPLVGRELL GWVGEFLTSA LGS
//
