ID A0A171A8Z1_9ACTN Unreviewed; 552 AA.
AC A0A171A8Z1;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=STXM2123_4339 {ECO:0000313|EMBL:GAT83638.1};
OS Streptomyces sp. F-3.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1840095 {ECO:0000313|EMBL:GAT83638.1, ECO:0000313|Proteomes:UP000078145};
RN [1] {ECO:0000313|EMBL:GAT83638.1, ECO:0000313|Proteomes:UP000078145}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Wang L.S., Gao P.J., Liu L., Zhang H.Q., Cheng Z., Sun X.M.;
RT "Streptomyces sp. F-3 geneom sequencing.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAT83638.1}.
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DR EMBL; BDDR01000030; GAT83638.1; -; Genomic_DNA.
DR RefSeq; WP_067397509.1; NZ_BDDR01000030.1.
DR AlphaFoldDB; A0A171A8Z1; -.
DR STRING; 1840095.STXM2123_4339; -.
DR OrthoDB; 291295at2; -.
DR Proteomes; UP000078145; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR InterPro; IPR006311; TAT_signal.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000078145};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..35
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 36..552
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023028712"
FT DOMAIN 84..130
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 225..374
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 377..551
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 367
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 454
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 552 AA; 57861 MW; 98FD1591E93AAC82 CRC64;
MTPRCTRPRR TALALATAVA TAALLGTGLS TGASAQPQAG STGAAPLAAA PVLLSDAERT
SLIRQAQADA SGTAREIGLG SQEKLVVKDV VKDADGTVHT RYERTYAGLP VLGGDLVVHT
SRTGRTQGVT RATEAAIEVD TLTPRITAAK AEKQAVTLAR AAGSENTTAD QAPRKVIWAA
DGTPVLAYET VVGGFQEDGT PNELHVITDA TTGEKLYEYQ GVVNGVGRTL YSGTVTLSTT
RSGSTYQLYD TTRGGHRTYN LARATSGTGT LFTDADDVWG TGTASSSSSD QTAAADAAYG
AQVTWDFYKN VFGRNGIRNN GTAAYSRVHY GNNYINAFWS DSCFCMTYGD GAGNVKPLTS
LDVAGHEMSH GLTSYTAGLR YSGESGGLNE ATSDIFGTGV EFYANNASDP GDYLIGEKID
INGNGTPLRY MDRPSKDGAS ADYWSSGVGN RDVHYSSGVA NHFFYLLAEG SGAKTINGVS
YNSPTYDGSR ITGIGRDKAL QIWYKALTTY MTSTTNYKGA RTATLNAAAA LYGSGSTEYN
TVAAAWTAVN VT
//
