ID A0A173KSE8_9SPHN Unreviewed; 402 AA.
AC A0A173KSE8;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=23S rRNA (Uracil(1939)-C(5))-methyltransferase {ECO:0000313|EMBL:ANI76600.1};
DE EC=2.1.1.190 {ECO:0000313|EMBL:ANI76600.1};
GN Name=rumA {ECO:0000313|EMBL:ANI76600.1};
GN ORFNames=EP837_00145 {ECO:0000313|EMBL:ANI76600.1};
OS Sphingobium sp. EP60837.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=1855519 {ECO:0000313|EMBL:ANI76600.1, ECO:0000313|Proteomes:UP000078304};
RN [1] {ECO:0000313|EMBL:ANI76600.1, ECO:0000313|Proteomes:UP000078304}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EP60837 {ECO:0000313|EMBL:ANI76600.1,
RC ECO:0000313|Proteomes:UP000078304};
RA Lee C.-M., Yoon S.-H., Kim B.-Y.;
RT "Complete genome sequence of a organophosphorus pesticides.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
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DR EMBL; CP015986; ANI76600.1; -; Genomic_DNA.
DR RefSeq; WP_066523761.1; NZ_CP015986.1.
DR AlphaFoldDB; A0A173KSE8; -.
DR STRING; 1855519.EP837_00145; -.
DR KEGG; sphb:EP837_00145; -.
DR PATRIC; fig|1855519.3.peg.151; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000078304; Chromosome 1.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS01230; TRMA_1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000078304};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT ACT_SITE 359
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 359
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 240
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 267
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 287
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 333
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 402 AA; 43416 MW; BF0F84C14508CB2A CRC64;
MTEIDTDIIV RIAAKGDGVT SDGRHVQLAA PGDRLTGDGR LLPGPHHITP PCMHFPACGG
CELQHVDEES FAAFATARVT GPLAGQGITA ETVLPAHVSP PRTRRRASLR AAREGRRIVI
GFAESGSHKL IDIAMCEILD PRLFALVQPL REVLPLILPD RRAAHVRMSL VDQGVDLLLE
RVRVEGLAAD EALRDFADTW KLARLTIDEG DGQQVRWEPE PVTVSFGGVA VGFPSFAFLQ
ATPDGEAALV EFVREALPGR GAVADLFCGL GTFALAIGEG RPVYAAEGAR DLVLSLKLAA
NRAQRRMVVD HRDLFRKPLT PEELNRFAAV VIDPPRAGAR EQVMQLAASA VPLIAYVSCN
PASFARDAAH LVAGGYVLES VKPVGQFRWS THVELAATFR RR
//
