ID A0A173MMJ4_9BACT Unreviewed; 675 AA.
AC A0A173MMJ4;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Oxygen sensor histidine kinase NreB {ECO:0000256|ARBA:ARBA00017322};
DE EC=2.7.13.3 {ECO:0000256|ARBA:ARBA00012438};
DE AltName: Full=Nitrogen regulation protein B {ECO:0000256|ARBA:ARBA00030800};
GN ORFNames=SAMN05421788_101164 {ECO:0000313|EMBL:SIS60344.1};
OS Filimonas lacunae.
OC Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae;
OC Filimonas.
OX NCBI_TaxID=477680 {ECO:0000313|EMBL:SIS60344.1, ECO:0000313|Proteomes:UP000186917};
RN [1] {ECO:0000313|Proteomes:UP000186917}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 21054 {ECO:0000313|Proteomes:UP000186917};
RA Varghese N., Submissions S.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Member of the two-component regulatory system NreB/NreC
CC involved in the control of dissimilatory nitrate/nitrite reduction in
CC response to oxygen. NreB functions as a direct oxygen sensor histidine
CC kinase which is autophosphorylated, in the absence of oxygen, probably
CC at the conserved histidine residue, and transfers its phosphate group
CC probably to a conserved aspartate residue of NreC. NreB/NreC activates
CC the expression of the nitrate (narGHJI) and nitrite (nir) reductase
CC operons, as well as the putative nitrate transporter gene narT.
CC {ECO:0000256|ARBA:ARBA00024827}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000256|ARBA:ARBA00000085};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; FTOR01000001; SIS60344.1; -; Genomic_DNA.
DR RefSeq; WP_076374737.1; NZ_FTOR01000001.1.
DR AlphaFoldDB; A0A173MMJ4; -.
DR STRING; 477680.SAMN05421788_101164; -.
DR KEGG; fln:FLA_4753; -.
DR OrthoDB; 617348at2; -.
DR Proteomes; UP000186917; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR CDD; cd16917; HATPase_UhpB-NarQ-NarX-like; 1.
DR Gene3D; 1.20.5.1930; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 2.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR011712; Sig_transdc_His_kin_sub3_dim/P.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR24421; NITRATE/NITRITE SENSOR PROTEIN NARX-RELATED; 1.
DR PANTHER; PTHR24421:SF65; SENSOR HISTIDINE KINASE COMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF07730; HisKA_3; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00028; TPR; 5.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF48452; TPR-like; 2.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:SIS60344.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000186917};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Two-component regulatory system {ECO:0000256|ARBA:ARBA00023012}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..675
FT /note="Oxygen sensor histidine kinase NreB"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011522658"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 582..668
FT /note="Histidine kinase"
FT /evidence="ECO:0000259|PROSITE:PS50109"
SQ SEQUENCE 675 AA; 77698 MW; D42E002FDE63BBBF CRC64;
MKNKLKAIYW LQFICLLIVF HFATAQNVPQ QNTATLPQLH DTAAINTMMQ KGYSIREQNT
DSSILLYTHT LNSSKLAGYN YGIGNSYLGL ARAHSILNKD QEAIAYSYQA LTYCTDTSNT
NKELRFNIYH CMAQVYYYLG KFDSCAWYRY QALHIVELNP DLSIQVQMRA YGSILQFWMN
VHNDIQHDRY IQSLWEHLNA IEKKAMAIGD STLLVSIYFQ KEAYYENIRL RDSSRYYCQL
TCEMGRRLHV TPSIVIASLM NTAISLLQDK QPDKALEYVN EGLKEMPLQN KANNRYYVFA
HFVLGEVYLQ QKKYQAAVNA LVPALKTAAA VHMIAYTDYA HKTLAQTYDA LKDYKNAGEH
WRLHAEILDS MQKDKKMELV YNVEMRYRIA DKEREIAQKE LSITRNKSRL RTQQFWIGGI
LCGTLLVLLV SLLLYRNSKH KHKLQTEKIR NLKQELQISS LQAMIAGEEK ERSRIARELH
DGLGGTLATI RTRLSYVFRK LTTSGESGHD LTEILQLLEE ASVELRKTAH NLMPEILLRE
GLAKASLLFC ERVRKGHMLE INTEIWGEVR RLPKDFELTA YRIIQELIHN TLKHARATQA
LVQIVFHESL LCITVEDNGT GITENNRQGE GMGLRTIEDR VKSLNGQMDI ASSPGKGTSI
YIELNIITTR QNILE
//
