ID A0A174KEC4_9FIRM Unreviewed; 341 AA.
AC A0A174KEC4;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000256|HAMAP-Rule:MF_00113};
DE EC=2.4.99.17 {ECO:0000256|HAMAP-Rule:MF_00113};
DE AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000256|HAMAP-Rule:MF_00113};
GN Name=queA {ECO:0000256|HAMAP-Rule:MF_00113,
GN ECO:0000313|EMBL:CUP07650.1};
GN ORFNames=DW021_04040 {ECO:0000313|EMBL:RHL49522.1}, DW222_02000
GN {ECO:0000313|EMBL:RHH21232.1}, DW272_01380
GN {ECO:0000313|EMBL:RHG19885.1}, DW723_05250
GN {ECO:0000313|EMBL:RHE76492.1}, DW767_03580
GN {ECO:0000313|EMBL:RHE14875.1}, DWW07_04040
GN {ECO:0000313|EMBL:RGV65730.1}, DWX77_00770
GN {ECO:0000313|EMBL:RGS76266.1}, DWY46_03945
GN {ECO:0000313|EMBL:RGR50544.1}, DXB38_07300
GN {ECO:0000313|EMBL:RGN87904.1}, DXB81_01855
GN {ECO:0000313|EMBL:RGN07300.1}, EAI82_06425
GN {ECO:0000313|EMBL:RYT67553.1}, ERS852394_00087
GN {ECO:0000313|EMBL:CUN38256.1}, ERS852533_00234
GN {ECO:0000313|EMBL:CUP07650.1};
OS Blautia obeum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Blautia.
OX NCBI_TaxID=40520 {ECO:0000313|EMBL:CUP07650.1, ECO:0000313|Proteomes:UP000095413};
RN [1] {ECO:0000313|Proteomes:UP000095409, ECO:0000313|Proteomes:UP000095413}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2789STDY5608837 {ECO:0000313|EMBL:CUN38256.1,
RC ECO:0000313|Proteomes:UP000095409}, and 2789STDY5834921
RC {ECO:0000313|EMBL:CUP07650.1, ECO:0000313|Proteomes:UP000095413};
RG Pathogen Informatics;
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000261105, ECO:0000313|Proteomes:UP000261222}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF14-23 {ECO:0000313|EMBL:RGV65730.1,
RC ECO:0000313|Proteomes:UP000265828}, AF21-24
RC {ECO:0000313|EMBL:RGS76266.1, ECO:0000313|Proteomes:UP000284242},
RC AF25-21 {ECO:0000313|EMBL:RGR50544.1,
RC ECO:0000313|Proteomes:UP000285839}, AF37-6AC
RC {ECO:0000313|EMBL:RHL49522.1, ECO:0000313|Proteomes:UP000285897},
RC AM18-2AC {ECO:0000313|EMBL:RHH21232.1,
RC ECO:0000313|Proteomes:UP000284024}, AM22-9LB
RC {ECO:0000313|EMBL:RHG19885.1, ECO:0000313|Proteomes:UP000284220},
RC AM27-32LB {ECO:0000313|EMBL:RHE76492.1,
RC ECO:0000313|Proteomes:UP000283928}, AM29-25AC
RC {ECO:0000313|EMBL:RHE14875.1, ECO:0000313|Proteomes:UP000284644},
RC OM03-6 {ECO:0000313|EMBL:RGN87904.1,
RC ECO:0000313|Proteomes:UP000261105}, and OM06-11AA
RC {ECO:0000313|EMBL:RGN07300.1, ECO:0000313|Proteomes:UP000261222};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:RYT67553.1, ECO:0000313|Proteomes:UP000293506}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=af_0058 {ECO:0000313|Proteomes:UP000293506}, and Af_0058
RC {ECO:0000313|EMBL:RYT67553.1};
RX PubMed=30630933; DOI=.1126/science.aau5238;
RA Jiang X., Hall A.B., Arthur T.D., Plichta D.R., Covington C.T., Poyet M.,
RA Crothers J., Moses P.L., Tolonen A.C., Vlamakis H., Alm E.J., Xavier R.J.;
RT "Invertible promoters mediate bacterial phase variation, antibiotic
RT resistance, and host adaptation in the gut.";
RL Science 363:181-187(2019).
CC -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the
CC 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC epoxyqueuosine (oQ-tRNA). {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-
CC methionine = adenine + epoxyqueuosine(34) in tRNA + 2 H(+) + L-
CC methionine; Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342, Rhea:RHEA-
CC COMP:18582, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708, ChEBI:CHEBI:57844,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:82833, ChEBI:CHEBI:194443;
CC EC=2.4.99.17; Evidence={ECO:0000256|HAMAP-Rule:MF_00113};
CC -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SIMILARITY: Belongs to the QueA family. {ECO:0000256|HAMAP-
CC Rule:MF_00113}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CYZD01000001; CUN38256.1; -; Genomic_DNA.
DR EMBL; CZBA01000001; CUP07650.1; -; Genomic_DNA.
DR EMBL; QSUB01000001; RGN07300.1; -; Genomic_DNA.
DR EMBL; QSUZ01000007; RGN87904.1; -; Genomic_DNA.
DR EMBL; QRUH01000002; RGR50544.1; -; Genomic_DNA.
DR EMBL; QRVV01000001; RGS76266.1; -; Genomic_DNA.
DR EMBL; QRZI01000002; RGV65730.1; -; Genomic_DNA.
DR EMBL; QSJW01000002; RHE14875.1; -; Genomic_DNA.
DR EMBL; QSKO01000005; RHE76492.1; -; Genomic_DNA.
DR EMBL; QRHZ01000001; RHG19885.1; -; Genomic_DNA.
DR EMBL; QRJH01000001; RHH21232.1; -; Genomic_DNA.
DR EMBL; QROS01000002; RHL49522.1; -; Genomic_DNA.
DR EMBL; RCXQ01000004; RYT67553.1; -; Genomic_DNA.
DR RefSeq; WP_055055219.1; NZ_WWVS01000004.1.
DR AlphaFoldDB; A0A174KEC4; -.
DR OrthoDB; 9805933at2; -.
DR UniPathway; UPA00392; -.
DR Proteomes; UP000095409; Unassembled WGS sequence.
DR Proteomes; UP000095413; Unassembled WGS sequence.
DR Proteomes; UP000261105; Unassembled WGS sequence.
DR Proteomes; UP000261222; Unassembled WGS sequence.
DR Proteomes; UP000265828; Unassembled WGS sequence.
DR Proteomes; UP000283928; Unassembled WGS sequence.
DR Proteomes; UP000284024; Unassembled WGS sequence.
DR Proteomes; UP000284220; Unassembled WGS sequence.
DR Proteomes; UP000284242; Unassembled WGS sequence.
DR Proteomes; UP000284644; Unassembled WGS sequence.
DR Proteomes; UP000285839; Unassembled WGS sequence.
DR Proteomes; UP000285897; Unassembled WGS sequence.
DR Proteomes; UP000293506; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051075; F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.10.240; QueA-like; 1.
DR Gene3D; 3.40.1780.10; QueA-like; 1.
DR HAMAP; MF_00113; QueA; 1.
DR InterPro; IPR003699; QueA.
DR InterPro; IPR042118; QueA_dom1.
DR InterPro; IPR042119; QueA_dom2.
DR InterPro; IPR036100; QueA_sf.
DR NCBIfam; TIGR00113; queA; 1.
DR PANTHER; PTHR30307; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR PANTHER; PTHR30307:SF0; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR Pfam; PF02547; Queuosine_synth; 1.
DR SUPFAM; SSF111337; QueA-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00113};
KW Glycosyltransferase {ECO:0000313|EMBL:RGN07300.1};
KW Isomerase {ECO:0000313|EMBL:CUP07650.1};
KW Queuosine biosynthesis {ECO:0000256|ARBA:ARBA00022785, ECO:0000256|HAMAP-
KW Rule:MF_00113}; Reference proteome {ECO:0000313|Proteomes:UP000095409};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_00113};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00113}.
SQ SEQUENCE 341 AA; 38638 MW; DE00538FA97D01DA CRC64;
MKTSDFYYDL PKELIAQDPL EDRSSSRLLH LSMQDGSFEH RHFTDILDYL HKGDCLVIND
TKVIPARLYG HKEETGAVIE ILLLKRREND IWECLVKPGK KARPGAKITF GDGILKGEII
DVVDEGNRLI QFHYDGIFEE ILDQLGEMPL PPYITHKLKD KNRYQTVYAK NDGSAAAPTA
GLHFTRELLK QVEDMGVKIA HVTLHVGLGT FRPVKVDDVE QHHMHSEFYM VEEDQAKLIN
DTKKNGGRVI SVGTTSCRTL ESATDENGIL QAGSGWTEIF IYPGYHFKMI DGLITNFHLP
ESTLLMLVSA LAGKEHIMAA YEEAVKERYR FFSFGDAMLI L
//
