ID A0A175YE12_DAUCS Unreviewed; 410 AA.
AC A0A175YE12;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=rRNA N-glycosylase {ECO:0000256|ARBA:ARBA00012001};
DE EC=3.2.2.22 {ECO:0000256|ARBA:ARBA00012001};
GN ORFNames=DCAR_029064 {ECO:0000313|EMBL:KZM81451.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZM81451.1, ECO:0000313|Proteomes:UP000077755};
RN [1] {ECO:0000313|EMBL:KZM81451.1, ECO:0000313|Proteomes:UP000077755}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DH1 {ECO:0000313|Proteomes:UP000077755};
RC TISSUE=Leaf {ECO:0000313|EMBL:KZM81451.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC adenosine on the 28S rRNA.; EC=3.2.2.22;
CC Evidence={ECO:0000256|ARBA:ARBA00000237,
CC ECO:0000256|RuleBase:RU004915};
CC -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC {ECO:0000256|RuleBase:RU004915}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZM81451.1}.
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DR EMBL; LNRQ01000009; KZM81451.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A175YE12; -.
DR EnsemblPlants; KZM81451; KZM81451; DCAR_029064.
DR Gramene; KZM81451; KZM81451; DCAR_029064.
DR Proteomes; UP000077755; Chromosome 9.
DR GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.420.10; Ricin (A subunit), domain 1; 2.
DR InterPro; IPR036041; Ribosome-inact_prot_sf.
DR InterPro; IPR001574; Ribosome_inactivat_prot.
DR InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR PANTHER; PTHR33453; -; 1.
DR PANTHER; PTHR33453:SF48; RRNA N-GLYCOSYLASE; 1.
DR Pfam; PF00161; RIP; 1.
DR SUPFAM; SSF56371; Ribosome inactivating proteins (RIP); 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU004915};
KW Plant defense {ECO:0000256|RuleBase:RU004915};
KW Protein synthesis inhibitor {ECO:0000256|RuleBase:RU004915};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755};
KW Toxin {ECO:0000256|RuleBase:RU004915}.
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 410 AA; 47877 MW; 6D9D077B9BDD9989 CRC64;
MTSTSQPTAV SSDEPLTLNL DKNEKDEYEK FIKSLIKEFP PCDVEKELRN FDKESADKEL
KSKIEKCFPG IPGNAASTRQ KESVRRTCSI EELKDKNWLL KVRLKHECRN LDILIKKNNL
YLVGYKGKYK EKEEDGAVNA ETEQADYWIV LTGKNHREVV KSFQKFPPSK ADRSAEKEEK
SKMVLARKGR DKERKITDAV TELKAMKEQI ERLKGKDNKD LIKRVDTLLA MVPRDITLER
VKELIGADDK AALDFLNYLK EPREDYNPER QHRKFIEQSV KVDNEIRRVK DRLAKVKLNR
RAFIKAVNYL TASSLDQEDR KFAKHIIKLA LMICEAVRFQ SIAEHVACNY YHKETHTDSN
LPENCITSIY YWGDRSALMQ RGGWYDAERR LLGENPTVIR QMDKDKRDKE
//
