ID A0A176Y9G6_9BRAD Unreviewed; 389 AA.
AC A0A176Y9G6;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=Mandelate racemase/muconate lactonizing protein {ECO:0000313|EMBL:OAE97724.1};
GN ORFNames=AYJ54_34760 {ECO:0000313|EMBL:OAE97724.1};
OS Bradyrhizobium centrolobii.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=1505087 {ECO:0000313|EMBL:OAE97724.1, ECO:0000313|Proteomes:UP000076959};
RN [1] {ECO:0000313|EMBL:OAE97724.1, ECO:0000313|Proteomes:UP000076959}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BR 10245 {ECO:0000313|EMBL:OAE97724.1,
RC ECO:0000313|Proteomes:UP000076959};
RA Simoes-Araujo J.L.Sr., Barauna A.C., Silva K., Zilli J.E.;
RT "Draft Genome Sequence of the Strain BR 10245 (Bradyrhizobium sp.) isolated
RT from nodules of Centrolobium paraense.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR633978-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR633978-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAE97724.1}.
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DR EMBL; LUUB01000124; OAE97724.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A176Y9G6; -.
DR STRING; 1505087.AYJ54_34760; -.
DR OrthoDB; 9802699at2; -.
DR Proteomes; UP000076959; Unassembled WGS sequence.
DR GO; GO:0008867; F:galactarate dehydratase activity; IEA:InterPro.
DR GO; GO:1990594; F:L-altrarate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03316; MR_like; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR033978; L-talarate_dehydratase.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR InterPro; IPR046945; RHMD-like.
DR PANTHER; PTHR13794; ENOLASE SUPERFAMILY, MANDELATE RACEMASE; 1.
DR PANTHER; PTHR13794:SF58; MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDF00134; L-talarate/galactarate_dehydra; 1.
DR SFLD; SFLDG00179; mandelate_racemase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR633978-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR633978-3}.
FT DOMAIN 168..264
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 189
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-1"
FT ACT_SITE 319
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-1"
FT BINDING 38..40
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 74..75
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 187
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 217
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 219
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 243
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 269
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 339
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT SITE 292
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-4"
SQ SEQUENCE 389 AA; 42891 MW; 49D6D9094B385EF1 CRC64;
MLANPLAAGL AVGEATTDRI AWVRTSLVFL PLAQAISDAK VLTGRQKPLT EIAFLFAEIE
TENGHSGIGY SYSKRAGGPG QFAHAREIAG ELIGEDPSDI ARLWDKLVWA GASVGRSGMS
TQAIAAFDIA LWDLKAKRAK LPLAKLLGAH RDSVRCYNTS GGYLSMPIEE VKERITMALE
KGIGAIKLKV GQPDPMVDLK RIEAVRNHIG DVPLAIDANQ QWDRTTALRF GRIAEQFNLT
WIEEPLDAYD YEGHAALAAE LAAPVATGEM LTSVDEHYEL IRNNSVDFIQ PDAPRVGGIT
PFLKIMALAD HKKLQLAPHY CMEIHLHLTA AYAREPWVEH FEWSEAMFNE KLEVMNGRMI
VPNRPGLGFS LSEQARAWAR DVHEVGKRP
//
