ID A0A177HRY1_9ACTN Unreviewed; 432 AA.
AC A0A177HRY1;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC {ECO:0000256|ARBA:ARBA00020092};
DE EC=2.7.7.43 {ECO:0000256|ARBA:ARBA00012491};
DE EC=3.1.3.45 {ECO:0000256|ARBA:ARBA00013066};
DE AltName: Full=KDO 8-P phosphatase {ECO:0000256|ARBA:ARBA00031051};
GN Name=neuA {ECO:0000313|EMBL:OAH13207.1};
GN ORFNames=STSP_35320 {ECO:0000313|EMBL:OAH13207.1};
OS Streptomyces jeddahensis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1716141 {ECO:0000313|EMBL:OAH13207.1, ECO:0000313|Proteomes:UP000077381};
RN [1] {ECO:0000313|EMBL:OAH13207.1, ECO:0000313|Proteomes:UP000077381}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G25(2015) {ECO:0000313|Proteomes:UP000077381};
RA Poehlein A., Roettig A., Hiessl S., Hauschild P., Schauer J., Madkour M.H.,
RA Al-Ansari A.M., Almakishah N.H., Steinbuechel A., Daniel R.;
RT "Genome sequence of Streptomyces sp. G25.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-deoxy-alpha-D-manno-2-octulosonate-8-phosphate + H2O = 3-
CC deoxy-alpha-D-manno-oct-2-ulosonate + phosphate;
CC Xref=Rhea:RHEA:11500, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:85985, ChEBI:CHEBI:85986; EC=3.1.3.45;
CC Evidence={ECO:0000256|ARBA:ARBA00000898};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acylneuraminate + CTP = a CMP-N-acyl-beta-neuraminate +
CC diphosphate; Xref=Rhea:RHEA:11344, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:60073, ChEBI:CHEBI:68671; EC=2.7.7.43;
CC Evidence={ECO:0000256|ARBA:ARBA00001862};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminate metabolism.
CC {ECO:0000256|ARBA:ARBA00005141}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the CMP-NeuNAc synthase family.
CC {ECO:0000256|ARBA:ARBA00010726}.
CC -!- SIMILARITY: Belongs to the KdsC family.
CC {ECO:0000256|ARBA:ARBA00005893}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAH13207.1}.
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DR EMBL; LOHS01000081; OAH13207.1; -; Genomic_DNA.
DR RefSeq; WP_067278501.1; NZ_LOHS01000081.1.
DR AlphaFoldDB; A0A177HRY1; -.
DR STRING; 1716141.STSP_35320; -.
DR PATRIC; fig|1716141.3.peg.3707; -.
DR OrthoDB; 9805604at2; -.
DR UniPathway; UPA00628; -.
DR Proteomes; UP000077381; Unassembled WGS sequence.
DR GO; GO:0019143; F:3-deoxy-manno-octulosonate-8-phosphatase activity; IEA:RHEA.
DR GO; GO:0008781; F:N-acylneuraminate cytidylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006054; P:N-acetylneuraminate metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd02513; CMP-NeuAc_Synthase; 1.
DR CDD; cd01630; HAD_KDO-like; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR003329; Cytidylyl_trans.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR010023; KdsC_fam.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR21485:SF5; 3-DEOXY-D-MANNO-OCTULOSONATE 8-PHOSPHATE PHOSPHATASE KDSC; 1.
DR PANTHER; PTHR21485; HAD SUPERFAMILY MEMBERS CMAS AND KDSC; 1.
DR Pfam; PF02348; CTP_transf_3; 1.
DR Pfam; PF08282; Hydrolase_3; 1.
DR SFLD; SFLDG01138; C1.6.2:_Deoxy-d-mannose-octulo; 1.
DR SFLD; SFLDS00003; Haloacid_Dehalogenase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipopolysaccharide biosynthesis {ECO:0000256|ARBA:ARBA00022985};
KW Nucleotidyltransferase {ECO:0000313|EMBL:OAH13207.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000077381};
KW Transferase {ECO:0000313|EMBL:OAH13207.1}.
SQ SEQUENCE 432 AA; 45498 MW; EAAFB816F7EC06F1 CRC64;
MTHPEAGTAA PVRRVLAVIP ARGGSKGVPA KNLAPVGGVP LVARAVRECR ATRLVTDVVV
STDDAAIAAC AREAGAEVVL RPAAIAGDTA TSEAAVLHAM DAHEALHGAA VDAVLLVQCT
SPFLVREDID GVAAAVVENG ADTAVTVAPF HGFIWRDADD EPAAIGTERA AVEGGTDTLV
ADPTTRGGYG VNHDKSFRPR RQDRPQDLLE TGAAYAMDAA GFREHKHRFF GHTELVRTDP
ARVLEVDDPH DLARARALAP LFDADRPGAL PTADDIDAVV LDFDGTQTDD RVLIDSEGRE
FVSVHRGDGL GIAALRRSGL TMLILSTEQN PVVAARARKL KIPVLHGIDR KDLALKQWCE
EQGIAPERVL YVGNDVNDLP CFALVGWPVA VASAHDVVRG AARAVTTVPG GDGAIREIAS
WILGPSLDSL NK
//
