ID A0A177NT35_9GAMM Unreviewed; 462 AA.
AC A0A177NT35;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE SubName: Full=Glucosylceramidase {ECO:0000313|EMBL:OAI21071.1};
GN ORFNames=A1359_02670 {ECO:0000313|EMBL:OAI21071.1};
OS Methylomonas lenta.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylomonas.
OX NCBI_TaxID=980561 {ECO:0000313|EMBL:OAI21071.1, ECO:0000313|Proteomes:UP000078476};
RN [1] {ECO:0000313|EMBL:OAI21071.1, ECO:0000313|Proteomes:UP000078476}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R-45370 {ECO:0000313|EMBL:OAI21071.1,
RC ECO:0000313|Proteomes:UP000078476};
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAI21071.1}.
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DR EMBL; LUUI01000022; OAI21071.1; -; Genomic_DNA.
DR RefSeq; WP_066977001.1; NZ_LUUI01000022.1.
DR AlphaFoldDB; A0A177NT35; -.
DR STRING; 980561.A1359_02670; -.
DR OrthoDB; 9806701at2; -.
DR Proteomes; UP000078476; Unassembled WGS sequence.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361188};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW Reference proteome {ECO:0000313|Proteomes:UP000078476};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 65..393
FT /note="Glycosyl hydrolase family 30 TIM-barrel"
FT /evidence="ECO:0000259|Pfam:PF02055"
FT DOMAIN 398..457
FT /note="Glycosyl hydrolase family 30 beta sandwich"
FT /evidence="ECO:0000259|Pfam:PF17189"
SQ SEQUENCE 462 AA; 50963 MW; CFF3CA5053945891 CRC64;
MSEKSNVLLP AESLVEDAYS LAVWLTCADQ SALFQRQAQD LRFSNISYDL PAIMVDAEQT
FQVMEGFGFS LTGGSAMLIS RLPDAVKNAL LQELFAPAAD GIGVSFLRLS IAASDLSERS
YSYDDRPDGQ ADFELAYFDI EAGDREVIPL LEEILAINPA VKIMATAWSA PRWMKTNQSF
VGGKLRPDCY GVYAAYLVKY LEAMRGRGIT IHAITPQNEP LNQKNDPSML MDASEQVEFI
KHHLGPTLAQ AGLDDVELFC WDHNCDVKAY PLTVFADAEA RQYLAGSAWH LYGGDISVLS
EVHQAYPEMK LYFTEQWVGS DGQFADDLWW HLRNVLIGSI RNWSQVVLEW NLASDPFCGP
HTKGGEARCV GALTIGDEVT RNVAYYVIAH AAKHVRPGSV RIYSDTQTAL ANVAFLTPSG
HVVLIVLNDG VQAESFCISY RNKNAVAHLS AGTVATYVWR AA
//
