ID A0A179EQE9_ENTTH Unreviewed; 558 AA.
AC A0A179EQE9;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs2 {ECO:0000313|EMBL:GEK36705.1};
GN Synonyms=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=A6E74_09215 {ECO:0000313|EMBL:OAQ55292.1}, ETH01_09920
GN {ECO:0000313|EMBL:GEK36705.1};
OS Enterococcus thailandicus.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=417368 {ECO:0000313|EMBL:OAQ55292.1, ECO:0000313|Proteomes:UP000078516};
RN [1] {ECO:0000313|EMBL:OAQ55292.1, ECO:0000313|Proteomes:UP000078516}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0711D 46 {ECO:0000313|EMBL:OAQ55292.1,
RC ECO:0000313|Proteomes:UP000078516};
RA Beukers A.G., Zaheer R., Goji N., Cook S.R., Amoako K., Chaves A.V.,
RA Ward M.P., Mcallister T.A.;
RT "Draft genome of an Enterococcus thailandicus strain isolated from bovine
RT feces.";
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GEK36705.1, ECO:0000313|Proteomes:UP000321361}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101867 {ECO:0000313|EMBL:GEK36705.1,
RC ECO:0000313|Proteomes:UP000321361};
RA Hosoyama A., Uohara A., Ohji S., Ichikawa N.;
RT "Whole genome shotgun sequence of Enterococcus thailandicus NBRC 101867.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAQ55292.1}.
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DR EMBL; BJUG01000004; GEK36705.1; -; Genomic_DNA.
DR EMBL; LWMN01000014; OAQ55292.1; -; Genomic_DNA.
DR RefSeq; WP_067484344.1; NZ_LWMN01000014.1.
DR AlphaFoldDB; A0A179EQE9; -.
DR PATRIC; fig|417368.6.peg.1593; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000078516; Unassembled WGS sequence.
DR Proteomes; UP000321361; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:OAQ55292.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000078516}.
FT BINDING 67..74
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 558 AA; 59515 MW; E4E6A00DA0C8BEC8 CRC64;
MTNLSDIEIA NQVEMNPITD IAEKLGIPKE SLALYGDYKA KINVSKLENL DKAKRGKLIL
VTAITPTPAG EGKTTTSVGL VDALTKIGKK AAIALREPSL GPVFGVKGGA AGGGYAQVVP
MEDINLHFTG DFHAIGAANN LLAALLDNHI HQGNALGIDS RRITWKRVVD MNDRQLRHIV
NGLQGRINGV PREDGYDITV ASEIMAVLCL ANNITDLKEK LKKIIVAYSF EGTPITAGDL
KAEGAMTALL KEAIQPNIVQ TLEHSPAFIH GGPFANIAHG CNSVLATKLA LQYADYAVTE
AGFGADLGAE KFIDIKCRLA ELKPDAVVLV ATIRALKMHG GMKKADLGTE SVEAVLKGLP
NLEKHLSNIQ EVYGMPVVVA INKFPTDTDA ELAAVEKACE ERNVEVTLSD VWAKGSEGGI
DLANKVIELA EKPNSFSHVY DLSDSIEEKL TKIVQRVYGG KGIELAPSAR KELAELEKLG
FTEYPICMAK TQYSFSDDAT LLGAPKDFTV TIRNLKVSAG AGFIVALTGA VMTMPGLPKS
PAAERIDVDA EGNISGLF
//
