ID A0A179ERP6_ENTTH Unreviewed; 254 AA.
AC A0A179ERP6;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=HAD family hydrolase {ECO:0000313|EMBL:OAQ55911.1};
DE SubName: Full=Haloacid dehalogenase {ECO:0000313|EMBL:GEK37448.1};
DE SubName: Full=TIGR01457 family HAD-type hydrolase {ECO:0000313|EMBL:ASZ08136.1};
GN ORFNames=A6E74_04105 {ECO:0000313|EMBL:OAQ55911.1}, CK496_09530
GN {ECO:0000313|EMBL:ASZ08136.1}, ETH01_17350
GN {ECO:0000313|EMBL:GEK37448.1};
OS Enterococcus thailandicus.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=417368 {ECO:0000313|EMBL:OAQ55911.1, ECO:0000313|Proteomes:UP000078516};
RN [1] {ECO:0000313|EMBL:OAQ55911.1, ECO:0000313|Proteomes:UP000078516}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0711D 46 {ECO:0000313|EMBL:OAQ55911.1,
RC ECO:0000313|Proteomes:UP000078516};
RA Beukers A.G., Zaheer R., Goji N., Cook S.R., Amoako K., Chaves A.V.,
RA Ward M.P., Mcallister T.A.;
RT "Draft genome of an Enterococcus thailandicus strain isolated from bovine
RT feces.";
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ASZ08136.1, ECO:0000313|Proteomes:UP000217197}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=a523 {ECO:0000313|Proteomes:UP000217197}, and A523
RC {ECO:0000313|EMBL:ASZ08136.1};
RA Ybazeta G., Douglas L., Graham J., Fraleigh N., Murad Y., Perez J.,
RA Diaz-Mitoma F., Nokhbeh R.;
RT "Whole genome sequencing and hybrid de novo assembly of Enterococcus
RT thailandicus a523.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:GEK37448.1, ECO:0000313|Proteomes:UP000321361}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101867 {ECO:0000313|EMBL:GEK37448.1,
RC ECO:0000313|Proteomes:UP000321361};
RA Hosoyama A., Uohara A., Ohji S., Ichikawa N.;
RT "Whole genome shotgun sequence of Enterococcus thailandicus NBRC 101867.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR000915-3};
CC Note=Divalent metal ions. Mg(2+) is the most effective.
CC {ECO:0000256|PIRSR:PIRSR000915-3};
CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. NagD family.
CC {ECO:0000256|ARBA:ARBA00006696}.
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DR EMBL; CP023074; ASZ08136.1; -; Genomic_DNA.
DR EMBL; BJUG01000009; GEK37448.1; -; Genomic_DNA.
DR EMBL; LWMN01000011; OAQ55911.1; -; Genomic_DNA.
DR RefSeq; WP_067482464.1; NZ_LWMN01000011.1.
DR AlphaFoldDB; A0A179ERP6; -.
DR GeneID; 77487881; -.
DR KEGG; eth:CK496_09530; -.
DR PATRIC; fig|417368.6.peg.2590; -.
DR OrthoDB; 9810449at2; -.
DR Proteomes; UP000078516; Unassembled WGS sequence.
DR Proteomes; UP000217197; Chromosome.
DR Proteomes; UP000321361; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07530; HAD_Pase_UmpH-like; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 2.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006357; HAD-SF_hydro_IIA.
DR InterPro; IPR006354; HAD-SF_hydro_IIA_hyp1.
DR InterPro; IPR023214; HAD_sf.
DR NCBIfam; TIGR01460; HAD-SF-IIA; 1.
DR NCBIfam; TIGR01457; HAD-SF-IIA-hyp2; 1.
DR PANTHER; PTHR19288; 4-NITROPHENYLPHOSPHATASE-RELATED; 1.
DR PANTHER; PTHR19288:SF46; HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF13344; Hydrolase_6; 1.
DR Pfam; PF13242; Hydrolase_like; 1.
DR PIRSF; PIRSF000915; PGP-type_phosphatase; 1.
DR SFLD; SFLDG01139; C2.A:_Pyridoxal_Phosphate_Phos; 1.
DR SFLD; SFLDS00003; Haloacid_Dehalogenase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:OAQ55911.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR000915-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000915-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000078516}.
FT ACT_SITE 9
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-1"
FT ACT_SITE 11
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-1"
FT BINDING 9
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
FT BINDING 11
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
FT BINDING 181
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-2"
FT BINDING 206
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
SQ SEQUENCE 254 AA; 27802 MW; C30F4B4E8261881F CRC64;
MSYKGYLIDL DGTIYRGTEA IPAGKRFVEE LQQRELPFLF VTNNTTKSPA TVAKRLADEF
DIHVAAETVY TASLATIDFM QTDGKGKKVF VIGESGLIDL ILEAGFEWEE ENPDYVVVGL
DNYLTYEKVV AATLAIQKGA TFIGTNPDKN IPTERGLLPG AGSVVSFVET ATQTTPIYIG
KPEAIIMDKA VEILGLQKEE VIMVGDNYET DIQAGIQNGI DTLLVLSGFT KAEDVPHLPV
PATFVKNSLD EWQF
//
