ID A0A179FU19_METCM Unreviewed; 1558 AA.
AC A0A179FU19;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=VFPPC_04933 {ECO:0000313|EMBL:OAQ68738.1};
OS Pochonia chlamydosporia 170.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Pochonia.
OX NCBI_TaxID=1380566 {ECO:0000313|EMBL:OAQ68738.1, ECO:0000313|Proteomes:UP000078397};
RN [1] {ECO:0000313|EMBL:OAQ68738.1, ECO:0000313|Proteomes:UP000078397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=170 {ECO:0000313|EMBL:OAQ68738.1};
RX PubMed=27416025;
RA Wang G., Liu Z., Lin R., Li E., Mao Z., Ling J., Yang Y., Yin W.B., Xie B.;
RT "Biosynthesis of antibiotic leucinostatins in bio-control fungus
RT Purpureocillium lilacinum and their inhibition on phytophthora revealed by
RT genome mining.";
RL PLoS Pathog. 12:E1005685-E1005685(2016).
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAQ68738.1}.
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DR EMBL; LSBJ02000003; OAQ68738.1; -; Genomic_DNA.
DR RefSeq; XP_018145588.1; XM_018284196.1.
DR STRING; 1380566.A0A179FU19; -.
DR GeneID; 28848190; -.
DR KEGG; pchm:VFPPC_04933; -.
DR OrthoDB; 1427975at2759; -.
DR Proteomes; UP000078397; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000078397};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 41..141
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 145..588
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 754..998
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1081..1542
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1558 AA; 175024 MW; 4B68934C55216A5C CRC64;
MSPGTMTTTE VYLLPLNDDG SPDVERQYIY IAPPSNDPVT LRFSIEGTSS ICRNGSLWVN
IPKKGKEFRR DSFREFKLTP DFNRSIEISI QIHQPGAYAF YTTYAELPSL EPHLSEDSSQ
KAVELKKTPV YYIDVAPRLR LDGRPLPLPA LSVFSVISKF MGKYPTDWDS HLRGISDRGY
NMIHFAPLQV RGSSNSPYSL YDQLGWDPEC FPHGERDVEK LVASLERDHS LLSLTDIVLN
HTAHNTKWLQ EHPEAGYNLS TAPWLESAYE LDSKLLELSA NLASLGLPVH PKSPEDLLLI
MEAIKKEVIA KIRLWEYYAL DVERDADAAV DAWTKTKTYT SQNTDGLQDE LEQLKDAGLA
QQVSFLKKFG LLGAGQLGER FLRSVDPNVA ATLLAFTFGR YEGESADSAD KAAARSKMVE
ILDAMNVPLY EEYDGDVKEI LEQLYNRIKY VRLDEHGPKL GPINDENPLI ETYFTRLPKN
AATTKHNPRD LMLVNNGWVW GGNALVDNAG PDARVYLRRE VIVWGDCVKL RYGSKPDDSP
WLWNHMTEYA RKLATHFAGL RIDNCHSTPI HVAEHILDEA RRVRPDLYVV AELFSGSEDT
DYVFVKRLGL SSLIREAMQA WSTAELSRLV HRHGGRPIGS FEVDEISRGD PTLSPGSQNK
KDLTREIIRR IKPTPVHALF MDCTHDNETP AQKRDARDTL PNAALVSMCS SATGSVMGYD
EVYPKLVDLV NETRLYTSES SRSAPIKVGR GKNGIAGVKK LLNQIHTLMG KDGYDETHIH
HEEEYITVHR VHPESRKGYF LIAHTAFPGY GNGNGALNPV HLAGTRAQHL GSWKLEVDDS
PEATAKAVNE GQFLVGLPSR VVDITGVDLD VQGEDTIITV RDKFPPGSIA LFETWIPAAE
HSAGLDTYVT SGAKAAWSQL DLIDLNFLMY RCAAEELDSN NGRDGTYDIP GYGTLVYAGL
QGWWSLMKDI IRENNLAHPL CQNLRQGTWA LDYILGRLQR MSETEGKEQL ARPASWLKER
FDAIRTIPSF LLPRYFGLVL RTAYNASRDR ALELMGENIE KGQWFLQSLA LVSVQQTGYV
KSASLWPNKA VPSVAAGLPH FAVEWSRCWG RDVFISIRGL YLGTGRFDEA KEHILAFASV
LKHGMIPNLL SSGDAPRYNS RDSIWFFLQT IQDYVHRAPE GTSLLKTSVK RRFLPYDDTW
FPTDDPRAYS KESTIEDIIQ EALQRHASGM KYREANAGPQ IDSQMKDEGF NQDIHVDWET
GIIFGGNQSN CGTWMDKMGE SERAGSKGVP GTPRDGAAIE ITGLLYSTLA WLVGLNEQGK
YAYSSVKTGD GKSVSFKDWA ERIKANFERC YFVPLTTEDD AKYNVNPAVI NRRGIYKDLY
KSGKEYEDYQ FRSNFPIAMA VAPSLFDPDH AMHALCLADS VLRGPTGMAT LDPADLNYRP
YYRNSEDSDD FATSKGRNYH QGPEWLWPTG FFLRALLKFD LMRRTTPEGR TEAFQQVTRR
LAGCKKMIQE SPWAGLTELT QKNGEFCPDS SPTQAWSAGC LIDLYMDGRE AQQEAAET
//
