ID A0A179SX96_9BACI Unreviewed; 1176 AA.
AC A0A179SX96;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=A6K24_23755 {ECO:0000313|EMBL:OAS85720.1};
OS Metabacillus litoralis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Metabacillus.
OX NCBI_TaxID=152268 {ECO:0000313|EMBL:OAS85720.1, ECO:0000313|Proteomes:UP000078534};
RN [1] {ECO:0000313|Proteomes:UP000078534}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C44 {ECO:0000313|Proteomes:UP000078534};
RA Lyu Z., Lyu W.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAS85720.1}.
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DR EMBL; LWSG01000019; OAS85720.1; -; Genomic_DNA.
DR RefSeq; WP_066333492.1; NZ_LWSG01000019.1.
DR AlphaFoldDB; A0A179SX96; -.
DR STRING; 152268.A6K24_23755; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000078534; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000078534}.
FT DOMAIN 637..798
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 819..973
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1176 AA; 134791 MW; FCD1B4FC97F9CC5C CRC64;
MNSLQQYFYD TDDFKTIVSG IEEGLKEQLV AGLSGSARTV FTAALYNDLK KSVLIVTHNL
YQAQKIYEDL VHLIVEDVYL YPVNDLIASE IAIASPELKA QRIEVLNRLA EGKRSIIVAP
VAGVRRLLPP TEIWKDKQLK LTLGQDIDLE EYIQQFISLG YERTDMVSSP GEFSVRGGII
DIYPLTEENA LRIELFDTEI DSIRSFNIDD QRSIHMLNEI SIGPAIEMIV NEDSKARSIQ
AVEFGLAKSL KKMKNEQQKE LLIENIQYDL ERLRNGQFSQ DIFKYSSLIY DKPTSLLDYF
SDNSIVILDE ISRIHETYDQ LDREEAEWYT SLLEDGKIFQ DVKMSHSYVD VVTKSKHPLI
YLSLFLRHVP YTSPQNILNL SCKQMQNFHG QMNVLKNELD RWKKSNYAVV FLGVNEERTK
KLEQVLEDYE INATILGDKE ALVSGKVCII EGDLQTGFEL PMQKLAVITE EELFKKRVKK
QVRRQKLSNA ERIKSYSELE IGDYVVHVNH GIGKYLGIET LEINGIHKDY LNIRYQGSDQ
LYVPVEQIDQ VQKYVGSEGK EPKIYKLGGS DWRRVKKKVE SSVQDIADDL IKLYAEREAS
EGYAFSPDGE MQKEFELAFP YQETEDQIRS IQEIKKDMER LRPMDRLLCG DVGYGKTEVA
IRAAFKAIAD GKQVALLVPT TILAQQHFET VRERFQEYPI KVGLLSRFRS RKEINETKKG
LSNGTVDMVI GTHRLLSKDV LYKDLGLLII DEEQRFGVTH KEKIKQLKSN VDVLTLTATP
IPRTLHMSML GVRDLSVIET PPENRFPVQT YVVEYNGALV RESIEREMAR GGQVFFLYNR
VEDIERKAEE ISMLVPDARV TYAHGKMTEN ELESVMLNFL EGEFDVLVST TIIETGVDIP
NVNTLIVNDA DKMGLSQLYQ LRGRVGRSNR VAYAYFTHRK DKVLTEVAEK RLQAIKEFTE
LGSGFKIAMR DLSIRGAGNL LGAQQHGFID SVGFDLYSQM LKEAIEERQT DPTKEKAIEV
EIDLQVDAYL PQEYITDGRQ KIDMYKRFRA ITSLEELEEL QEEIIDRFGE YPVEVSYLFQ
IARIKVFAIQ ERVELIKQDK DVINILIEEG ASNKIDGHRL FELSNRYNRI VGLGMDGSRL
KLTIATKGLA VPKWLDIINE LLNGLAQVKK EEMTLN
//
