ID A0A182UAI3_9DIPT Unreviewed; 1370 AA.
AC A0A182UAI3;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
OS Anopheles melas.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=34690 {ECO:0000313|EnsemblMetazoa:AMEC016895-PA, ECO:0000313|Proteomes:UP000075902};
RN [1] {ECO:0000313|Proteomes:UP000075902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM1001059 {ECO:0000313|Proteomes:UP000075902};
RG The Broad Institute Genomics Platform;
RA Neafsey D.E., Besansky N., Howell P., Walton C., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Anopheles melas CM1001059_A (V2).";
RL Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:AMEC016895-PA}
RP IDENTIFICATION.
RC STRAIN=CM1001059 {ECO:0000313|EnsemblMetazoa:AMEC016895-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR STRING; 34690.A0A182UAI3; -.
DR EnsemblMetazoa; AMEC016895-RA; AMEC016895-PA; AMEC016895.
DR VEuPathDB; VectorBase:AMEC016895; -.
DR Proteomes; UP000075902; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0031981; C:nuclear lumen; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR CDD; cd02583; RNAP_III_RPC1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR035697; RNAP_III_RPC1_N.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 251..556
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 1370 AA; 152814 MW; 964329CD54561DA1 CRC64;
MPKEQFREAD VIKKISHVSF NIDSPQQIQQ EAHIHVVAKN LYNQNADRTP VAYGVLDRRM
GVSQKDASCE TCKKGLNDCV GHFGYIDLAL PVFHVGHFRA TITILQSICK VCSRVMLKEE
EKKNFSIRLV NPNLSYLAKK SIHSQVLKKA KKNTKCPYCN SLNGPVKKGP GLMKIVHEPF
RGKKPSDPLV TNALHEMQAA VESNRELAQT VVGPSSLSKE LNPVEVLDLF RNIPKSDVPL
LGMTSTYSNP ADLIVTRVFV PPVCIRPSVV SEVKAGTTED DLTMKQSEIL LISDVITKHM
MTNGKIELIQ EDWDYLQLHC ALYFNSELSG IPLSLMPKKP TRGIVQRLKG KQGRFRGNLS
GKRVDFSART VISPDPNLMI HQVGVPDRVA KILTFPERVN LANITRMRQL IRNGTEKHPG
ANYVQQKGSA FKKYLAYGNR DKVAQDLKCG DIVERHLIDG DVVLFNRQPS LHKLSIMCHV
AKVQPQRTFR FNECACTPYN ADFDGDEMNL HLPQTEEARA EALVLMGNKS NLVTPRNGEL
LIAATQDFIT GGYLLTQKDQ FLTLEQVMQL AACMLSGPDS NMVIDLPRPA ILKPRKLWTG
KQIFSLILKP NSQSCVNANL YTKGRNYTRG NDMCVKDSWV IIRNSQLLCG AMDKGTLGSG
TKNCIFYVIL RDFGEEYAIK SMWRLARMAS YFMMNHGFSF GIGDVTPSRK LLEEKQKLLE
NGYRKCDEYI AEMKKGTLQC QPGCTAEQTL EAVMLKELSS IRELAAKACF RELHPTNSAL
IMAQSGSKGS NINISQMIAC VGQQAINGKR VPNGFEDPKI PAARGFVQNS FYSGLTPTEF
FFHTMAGREG LVDTAVKTAE TGYLQRRLVK CLEDLVVQYD GTVRNAIGEV VEFSYGADGL
DPVYMEVKNK PVDIERQFIH VRNMLPCRDE IPLRGEEVLS EGERILSKPQ FSNCRQDFRK
ECLGFLEKFG RRLCTLQRLY RNGPRVTTEV QRTTAGHLEE FLLQVRNKYN KAVTEPGTAV
GALAAQSIGE PGTQMTLKTF HFAGVASMNI TQGVPRIVEI INATKAISTP IITAEIENNT
NMEFARKVKA RIEKTTLGEI SSYIEEVYKM NECFLLIKLD HGRIRVLGLE VNADTIRYSI
CTSKLKLKQE NVEVYGPSLI IIRPDSSKHS HSLNAELQRL ITAIPNVVVA GLPQVSRAVI
AVDDSRGTPK YRLCVEGCGL RDVMGTYGVI GNRTKSNNIL EVYQTLGIEA ARTTIMTEIT
EVMEGHGMSV DHRHIMLLSG QMTSRGEVLG ITRHGLAKMR ESVFNLASFE KTADHLFDAA
YYGQTDSVDG VSERIILGMP ASIGTGLFKL IHNHSNTQLE SISCPIFNVV
//
