ID A0A182UAI9_9DIPT Unreviewed; 794 AA.
AC A0A182UAI9;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=ribonuclease Z {ECO:0000256|ARBA:ARBA00012477};
DE EC=3.1.26.11 {ECO:0000256|ARBA:ARBA00012477};
OS Anopheles melas.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=34690 {ECO:0000313|EnsemblMetazoa:AMEC016901-PA, ECO:0000313|Proteomes:UP000075902};
RN [1] {ECO:0000313|Proteomes:UP000075902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM1001059 {ECO:0000313|Proteomes:UP000075902};
RG The Broad Institute Genomics Platform;
RA Neafsey D.E., Besansky N., Howell P., Walton C., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Anopheles melas CM1001059_A (V2).";
RL Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:AMEC016901-PA}
RP IDENTIFICATION.
RC STRAIN=CM1001059 {ECO:0000313|EnsemblMetazoa:AMEC016901-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of RNA, removing extra 3' nucleotides
CC from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy
CC group is left at the tRNA terminus and a 5'-phosphoryl group is left
CC at the trailer molecule.; EC=3.1.26.11;
CC Evidence={ECO:0000256|ARBA:ARBA00000402};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the RNase Z family.
CC {ECO:0000256|ARBA:ARBA00007823}.
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DR AlphaFoldDB; A0A182UAI9; -.
DR STRING; 34690.A0A182UAI9; -.
DR EnsemblMetazoa; AMEC016901-RA; AMEC016901-PA; AMEC016901.
DR VEuPathDB; VectorBase:AMEC016901; -.
DR Proteomes; UP000075902; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0042781; F:3'-tRNA processing endoribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07718; RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 2.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR InterPro; IPR047151; RNZ2-like.
DR InterPro; IPR027794; tRNase_Z_dom.
DR PANTHER; PTHR12553; ZINC PHOSPHODIESTERASE ELAC PROTEIN 2; 1.
DR PANTHER; PTHR12553:SF49; ZINC PHOSPHODIESTERASE ELAC PROTEIN 2; 1.
DR Pfam; PF12706; Lactamase_B_2; 1.
DR Pfam; PF13691; Lactamase_B_4; 1.
DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 41..92
FT /note="tRNase Z endonuclease"
FT /evidence="ECO:0000259|Pfam:PF13691"
FT DOMAIN 496..709
FT /note="Metallo-beta-lactamase"
FT /evidence="ECO:0000259|Pfam:PF12706"
FT REGION 163..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 794 AA; 89793 MW; 6FEE7C4516EC44E5 CRC64;
MPLDPKHIAE AQKQRLKLKQ KVSKVSPGIV NLQVLGCGAP GTPASVYLFT DQTRYLFNCG
EGTQRLAYEH KTKLSCLENI FMTRTNWERI GGLPGICLTM QDVGVPAVSL HGPPGLDELF
KAMRRFVILK DMKVEASEYA TGDVYEDHVM TLRYVVINRQ PASATREAGS DEDQNQDEAT
VDDTDYYAYE RKRETLQKPQ SEAAKTVQST NWTKREESSV MAYICKLKPR HGQLSLEQCV
ELGVPPGPLL GQLKNGNDVT LPDGRVVRSC EVRAPDDPGP VFMFIDIPSR EYMDDFVAKA
ELFEKYQQTA VEESDQAIFV VHFSPLDVMR CDEYRQFMDR FSASTRHIAL NEVNSFSGYI
AAHRIQYHLN QLDGQIFPLL REENNQYADP PANDTDLVRS SSLSYMHIRP PKGIDRTLEA
SLNPQEYLNE LELLPDFKEA LAELKQQLAQ HTVRRSAAVR AEQFPRLIFL GTGSSIPNKT
RNVSAILILT SKQSSILLDC GEGTVGQIWR VFGKEQAEEI LRSIKMVYIS HLHADHHLGL
IGLLQARKKL LGDNCERLTL VAPEQISYWL RLYDCRFETI HRDYVLVKNA DLLENPLQDE
KLLAMGIKEI ATCRVRHCPH SFGVALKVAS LGTHPETNIE GDVKITYSGD TMPCESLIEL
GRDSTVLIHE ATMEDELSAE ARIKMHSTLS QAIEQGRKMN ARYTLLTHFS QRYAKIPRLR
PEQQQAGLGT DLGIAFDNME VTLDDLPTLC KFYPALKAMF ISHFEEMEQK AIKRGNKKLR
LETATRSKEC SPTR
//
