ID A0A182XMQ5_ANOQN Unreviewed; 1042 AA.
AC A0A182XMQ5;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2023, sequence version 2.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Aminopeptidase {ECO:0008006|Google:ProtNLM};
OS Anopheles quadriannulatus (Mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=34691 {ECO:0000313|EnsemblMetazoa:AQUA011151-PA, ECO:0000313|Proteomes:UP000076407};
RN [1] {ECO:0000313|Proteomes:UP000076407}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SANGQUA {ECO:0000313|Proteomes:UP000076407};
RG The Broad Institute Genomics Platform;
RA Neafsey D.E., Howell P., Walker B., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Anopheles quadriannulatus QUAD4_A.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:AQUA011151-PA}
RP IDENTIFICATION.
RC STRAIN=SANGQUA {ECO:0000313|EnsemblMetazoa:AQUA011151-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR AlphaFoldDB; A0A182XMQ5; -.
DR STRING; 34691.A0A182XMQ5; -.
DR EnsemblMetazoa; AQUA011151-RA; AQUA011151-PA; AQUA011151.
DR VEuPathDB; VectorBase:AQUA011151; -.
DR Proteomes; UP000076407; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR PANTHER; PTHR11533:SF174; PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT DOMAIN 188..372
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 407..624
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 704..1017
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 480
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 479
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 483
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 502
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 565
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1042 AA; 117241 MW; 0D098AD76CE7D3B9 CRC64;
MRLLYGNRRL APAVCVQYFQ HTTALAAPQA AAPRPYSAGR WNDSVATAAK QCYLFNSPSA
VTSPASAAPA TVGLTSAWQR YRLREQRNTP PRSRVSACVQ RQQSSSGCYV HFAPKQSSGV
WSTVVRPVVY GCDGNSARTV SCCARETQRR YSSTSATIDG SDVCETSEYK MSATTGKPKF
QRLPTNVVPE HYRLTLKPNL TALTFEGNTA VELKVVEATD RITLNALDLK LGTATVSFGD
QQLTAQDIQF DAGQETACFV FGAEIPPGKA TLAVEFSGEL NDKMKGFYRS KYFSPTGEER
YAGVTQFEAT DARRCFPCWD EPAIKATFDI SLIVPTNLVA LSNMPVVEER PEPADNTVHF
KFDRTPVMST YLVAVVVGEY DYVEDRSADG VLVRVYTPVG KREQGRFALD VATKVLPYYK
DYFNIAYPLP KMDLIAISDF SAGAMENWGL ITYRETFVLV DPENTSLIRK QSIALTVGHE
IAHQWFGNLV TMEWWTHLWL NEGYASFVEF LCVDHLFPDY DIWTQFVTDM YTRALELDCL
RNSHPIEVPV GHPSEIDEIF DEISYNKGAS VIRMLHHYIG DEDFKRGMNL YLTRHQYNNT
RTEDLWNALQ EASSKPVGAV MSTWIQRMGF PVVQVRSSKQ LEGNRRVLSI AQSKFCADGC
EAPEQSLWMI PINVSTPSSG NAVSTVLETA TADITVEGVG EQDWVKINPG TIGYYRTQYP
AEMLEQFLPA IKNMTLPPLD RLGLIDDLFA LVQAGKSSTV DALKVIDAYR NENNYTVWSS
ISNCLAKLQL LLAHTPAEKQ FSEYGVRLYQ PVAEKLGWDV KPGESHLDTL LRSLVLGRLV
SFGCPKTVAE AKRRFEEHAQ NKSVLPADLR STCYRAVLQH GDLATYDEML RLYRATDLHE
EKDRISRALG SIGNVDILRK VIDFAMSEEV RAQDSVFVIV SVAINPKGRD MAWDYFCEHW
QVLLNQYEGG FLLARLIKYL TENFSTEERA KEVEQFFREH DFPGTERTVS QSIETIRLNA
DWMRRDLDAI SAYLKQQEAE GH
//
