ID A0A182XV12_ANOST Unreviewed; 1876 AA.
AC A0A182XV12;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 08-NOV-2023, entry version 37.
DE RecName: Full=uS12 prolyl 3-hydroxylase {ECO:0000256|ARBA:ARBA00029938};
OS Anopheles stephensi (Indo-Pakistan malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=30069 {ECO:0000313|EnsemblMetazoa:ASTEI00048-PA, ECO:0000313|Proteomes:UP000076408};
RN [1] {ECO:0000313|Proteomes:UP000076408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Indian {ECO:0000313|Proteomes:UP000076408};
RX PubMed=25244985; DOI=10.1186/preaccept-1262842421127991;
RA Jiang X., Peery A., Hall A.B., Sharma A., Chen X.G., Waterhouse R.M.,
RA Komissarov A., Riehle M.M., Shouche Y., Sharakhova M.V., Lawson D.,
RA Pakpour N., Arensburger P., Davidson V.L., Eiglmeier K., Emrich S.,
RA George P., Kennedy R.C., Mane S.P., Maslen G., Oringanje C., Qi Y.,
RA Settlage R., Tojo M., Tubio J.M., Unger M.F., Wang B., Vernick K.D.,
RA Ribeiro J.M., James A.A., Michel K., Riehle M.A., Luckhart S.,
RA Sharakhov I.V., Tu Z.;
RT "Genome analysis of a major urban malaria vector mosquito, Anopheles
RT stephensi.";
RL Genome Biol. 15:459-459(2014).
RN [2] {ECO:0000313|EnsemblMetazoa:ASTEI00048-PA}
RP IDENTIFICATION.
RC STRAIN=Indian {ECO:0000313|EnsemblMetazoa:ASTEI00048-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + [ribosomal protein uS12]-L-proline + O2 =
CC [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + CO2 + succinate;
CC Xref=Rhea:RHEA:54156, Rhea:RHEA-COMP:13816, Rhea:RHEA-COMP:13818,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:50342, ChEBI:CHEBI:85428;
CC Evidence={ECO:0000256|ARBA:ARBA00001792};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis. {ECO:0000256|ARBA:ARBA00004687}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the PIGS family.
CC {ECO:0000256|ARBA:ARBA00005316}.
CC -!- SIMILARITY: Belongs to the TPA1 family.
CC {ECO:0000256|ARBA:ARBA00007443}.
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DR STRING; 30069.A0A182XV12; -.
DR EnsemblMetazoa; ASTEI00048-RA; ASTEI00048-PA; ASTEI00048.
DR VEuPathDB; VectorBase:ASTE007159; -.
DR VEuPathDB; VectorBase:ASTEI00048; -.
DR VEuPathDB; VectorBase:ASTEI20_032120; -.
DR VEuPathDB; VectorBase:ASTEI20_038540; -.
DR UniPathway; UPA00196; -.
DR Proteomes; UP000076408; Unassembled WGS sequence.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016301; F:kinase activity; IEA:InterPro.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:InterPro.
DR GO; GO:0031543; F:peptidyl-proline dioxygenase activity; IEA:UniProt.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; IEA:InterPro.
DR Gene3D; 2.60.200.40; -; 1.
DR Gene3D; 2.60.120.620; q2cbj1_9rhob like domain; 2.
DR InterPro; IPR017438; ATP-NAD_kinase_N.
DR InterPro; IPR045363; CERK_C.
DR InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR InterPro; IPR016064; NAD/diacylglycerol_kinase_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR019601; Oxoglutarate/Fe-dep_Oase_C.
DR InterPro; IPR006620; Pro_4_hyd_alph.
DR InterPro; IPR019540; PtdIno-glycan_biosynth_class_S.
DR InterPro; IPR039558; TPA1/OFD1_N.
DR PANTHER; PTHR21072; GPI TRANSAMIDASE COMPONENT PIG-S; 1.
DR PANTHER; PTHR21072:SF13; GPI TRANSAMIDASE COMPONENT PIG-S; 1.
DR Pfam; PF13661; 2OG-FeII_Oxy_4; 1.
DR Pfam; PF19280; CERK_C; 1.
DR Pfam; PF00781; DAGK_cat; 1.
DR Pfam; PF10637; Ofd1_CTDD; 1.
DR Pfam; PF10510; PIG-S; 1.
DR SMART; SM00046; DAGKc; 1.
DR SMART; SM00702; P4Hc; 1.
DR SUPFAM; SSF111331; NAD kinase/diacylglycerol kinase-like; 1.
DR PROSITE; PS50146; DAGK; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW GPI-anchor biosynthesis {ECO:0000256|ARBA:ARBA00022502};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000076408};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
SQ SEQUENCE 1876 AA; 209795 MW; A92C52E4111D4D78 CRC64;
MKPTDKRRAH GDDADDGGGT AEQSDRSGST AREDGKALLR ILTRFHIFHI ALNNPRASLH
ASENAPTPPA AKRTKRGTIV DDSNTELVSI NGCFFDDDFR ERFKVAWQRK ESIAGNNYQL
KQDPFQLAVF QHFLQEDVNR ADATKRLEAE FSTVDWKRKQ MDLYEFYQTN DLCSLERDYL
QAFYTVLKEQ MMPLVEELTG IKLTHVSASC SMYNAGDYLL VHDDLLSDRR IAYVFYLSPW
DCHREWCEKD GGALELFKAD GNQLPVFPVT DKIYPQNNQL VLFRVCEKSF HQVGEVTTFD
YPRLTINGWF HGPTAPAAQC AGKTSSNSVP QPPAIIVDTH YLSPRKVDID LAGWINDVYL
EDEVKQNVQK KVEMFSEVSL EQFLLLPRFS VLLETLCNVP NLEWKIKGPA HLRKYEVLNF
KTLPTDASPL GELYDLFTSR TMFRLLYDYT ELDLYGRKAK APKCAIELQR WERGCYTVLG
DSSTYADSTL DLTFYLNAQE NVGVITYLVP EADQQQQQQQ QGSSGHDQPS SSSSAAYAEC
YDEDPVLLTL LPKDNVLNVV YRAEGTTKFT KYVSHNTHLE RANASSTGHA YTYILVCTAA
IHIYATLAFI VIIIVIGVPM WWKTTEVYRV SLPYSEIRAL NEEPIRAAFR LGLYCPSAER
REILAFELRK KFEQNFVFEL DLELLSLDAA TVASIKTPAR LEAELLHRYP TSAGQFLFIE
WHKLDDDILV TSDRTAFISE SASSLKAYQV LSSWILQEYK LKAILGSRDA QQTLRGRQLR
LNSAPLQGQY EVLITVLNPR PDLQKVHWNA RAAAETYIEP FLNELSGITN FTIKTQWIYQ
VGIVEGASTQ PKQVPDDTKL GRHYALAEDS LPHIITSLEK KLGTQITDHP CIHLVVYVPP
CAQAPLKIYR RDGQRAPPAS GGSVEAFTSA KWGGIVFANP AEATCVRYME SEQFSDVYLH
AQDVMPVLLY QLRKIFDLEN NTPLLGGTLV PYSTIEPRLW EVDTFVRTNT IYLVHSATTT
LQSLIQLLGG IEYIVINDEV GAAIQNAYQK IVEAKQQLAA GSLQRAALLA REAYASAERA
FFDPSMLALL YFPNEQKYAI YIPLFLPIMI PVVFSFNTII KYFRGKSGPA SAKTKHDFIS
SLLFSGKAIP QQCRANLEQN PSAVQRATNG TARSPQSSSK VSSSVSEAAC HTLCATGSEQ
HQRLAVHRQI IQALCKLPER SKQGFPPSWW PSSCVAFFVR VSAMDKEQEV LLNAFVIAKK
RYRVFYNAGI LVWEDESSKK AKTTVPITDV LSVAVSKHPH ANGKQWMGKR SSKAPPPPSP
TAPPIARNDA HGSLDSDRIP VLSGSTTTNT LATNASNNIP HHHSNRGSLN ELSDSHDDRR
DTSDYFDGGI QAGNFNYLIL HYAALVCAKT SRWRVKSVAL HNSEQRIVEL WYNRLSSDLR
DQNRPKNLLL FLNPYGGKKN ALALYERFAK PLFRLAQVDI NLIITQRAQQ IYDIVTSKSI
VLDNYDGLVC CGGDGTFAEL FNGLVTRTMM DCGIDVKHPP FLPKPNIPIG IIPAGSTDTV
ACCLNGTTDI KTSIIHIILG QHSGLDISAV YGADAVAQDD DGSPSLGGKP RRPELLKLFA
SALSYGYLGD IAYDSEKYRW MGPKRYDYSG FKKFLANRGY SAEIVVHLDR RGKQDPNDGV
RCLDQCSRCK KALCSSQVGS EAGAEPDDTE PLVVRGKFLM VSGANISCSC ERSPQGFSPF
CHLGDGLLDL VLVRHTSMLN NLRLLLTMTS KTKKLSELPF VEVYRTKEFR FTANGQQQQQ
GDTNGGEVAP ALSSEMLKRR SKWNCDGEVL LDTNIIVESN CQLIRVFRRG ILPSGGHGGN
TLDTDDKDAC CFGLCN
//
