ID A0A182Y986_ANOST Unreviewed; 893 AA.
AC A0A182Y986;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 13-SEP-2023, entry version 33.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS Anopheles stephensi (Indo-Pakistan malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=30069 {ECO:0000313|EnsemblMetazoa:ASTEI05022-PA, ECO:0000313|Proteomes:UP000076408};
RN [1] {ECO:0000313|Proteomes:UP000076408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Indian {ECO:0000313|Proteomes:UP000076408};
RX PubMed=25244985; DOI=10.1186/preaccept-1262842421127991;
RA Jiang X., Peery A., Hall A.B., Sharma A., Chen X.G., Waterhouse R.M.,
RA Komissarov A., Riehle M.M., Shouche Y., Sharakhova M.V., Lawson D.,
RA Pakpour N., Arensburger P., Davidson V.L., Eiglmeier K., Emrich S.,
RA George P., Kennedy R.C., Mane S.P., Maslen G., Oringanje C., Qi Y.,
RA Settlage R., Tojo M., Tubio J.M., Unger M.F., Wang B., Vernick K.D.,
RA Ribeiro J.M., James A.A., Michel K., Riehle M.A., Luckhart S.,
RA Sharakhov I.V., Tu Z.;
RT "Genome analysis of a major urban malaria vector mosquito, Anopheles
RT stephensi.";
RL Genome Biol. 15:459-459(2014).
RN [2] {ECO:0000313|EnsemblMetazoa:ASTEI05022-PA}
RP IDENTIFICATION.
RC STRAIN=Indian {ECO:0000313|EnsemblMetazoa:ASTEI05022-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR AlphaFoldDB; A0A182Y986; -.
DR STRING; 30069.A0A182Y986; -.
DR EnsemblMetazoa; ASTEI05022-RA; ASTEI05022-PA; ASTEI05022.
DR VEuPathDB; VectorBase:ASTE002205; -.
DR VEuPathDB; VectorBase:ASTEI05022; -.
DR VEuPathDB; VectorBase:ASTEI20_044091; -.
DR OMA; HDLGAPN; -.
DR Proteomes; UP000076408; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 2.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000076408}.
FT DOMAIN 86..388
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 446..881
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 893 AA; 102159 MW; 8C97CCB07B4BD5EE CRC64;
MDGDSVQSMS DFKQQVAAVP QYGLKLKFNH VFSDTRNQNL RPSIRQLLPI VPMALRYIPY
YWKVSREGRQ VLMDYWYTEN AKQIYGAPLG GIGAGTIGRG FAGEFCRYQL KPGLYEYNTV
HANQFIVTIK DEAGATIFHS LLSTYSRPKN PLGSWESALD ASRCSYTALY PRAWSEYDLS
EHGIRLVQRQ ISPIIPHDYK ESSLPCGVFV WTVENVCDKD RQVTLTFTFK NGTGTKKQDA
EGGSETSAFT QGNARGVSIR QTIADMQCTY CVSCRGSSEI NLTRCERFDP TGNGEKLWND
LKENGHLTEK SNDETLKSKD VAVAVSGQIL VQPGTTSQLE FALVWDMPKV HFQKRGKEYH
RYYTKYFGKS GDAGPMISDY ALNNYGKWER LIDEWQRPIL EDADLPDWYK SAIFNELYFI
ADGGSVWLTM DDQSDLPYDD PRLAYGRYSY LEGHEYRMYT TYDVHFYASH AIASLWPNLQ
VSIQYDYKDS IEREITEGRK HLYDGKVIPR KIKNSVPHDL GDPAEEPFDL INAYPIHDVS
EWRDLNIKFI LQVYRDYYTL NHYAQLNAEN ASKFSSIEFI DKESMYDTYI QDNRNKPNGG
SSPTDNKAAN RKSASMYINE TNGKVYLMDA LTYLKAMYPA CRTVLERSLE WDKDDDGLIE
NSKSPDQTYD TWVMDGPSAY CGGLWLASLH CMSTMASLLD QSEDSDRYKA ILDKGRASFE
EKLWNGTYYR FDAQSGSKNS IMSDQLCGHW YLRACGFDYD VFPKENVRLA MRTIYENNVM
RFCGGQLGAV NGYVPSGQPN KEGRPDTSTI QGEEVWTGVT YALASTMIHE GMFEEAFQTA
GGLYQALSER IGMNFETPEA VYAERHYRAI GYMRPLSIWS MQTAWEMKKL RRD
//
