ID A0A182YDX4_ANOST Unreviewed; 1056 AA.
AC A0A182YDX4;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Aminopeptidase {ECO:0000313|EnsemblMetazoa:ASTEI06660-PA};
OS Anopheles stephensi (Indo-Pakistan malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=30069 {ECO:0000313|EnsemblMetazoa:ASTEI06660-PA, ECO:0000313|Proteomes:UP000076408};
RN [1] {ECO:0000313|Proteomes:UP000076408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Indian {ECO:0000313|Proteomes:UP000076408};
RX PubMed=25244985; DOI=10.1186/preaccept-1262842421127991;
RA Jiang X., Peery A., Hall A.B., Sharma A., Chen X.G., Waterhouse R.M.,
RA Komissarov A., Riehle M.M., Shouche Y., Sharakhova M.V., Lawson D.,
RA Pakpour N., Arensburger P., Davidson V.L., Eiglmeier K., Emrich S.,
RA George P., Kennedy R.C., Mane S.P., Maslen G., Oringanje C., Qi Y.,
RA Settlage R., Tojo M., Tubio J.M., Unger M.F., Wang B., Vernick K.D.,
RA Ribeiro J.M., James A.A., Michel K., Riehle M.A., Luckhart S.,
RA Sharakhov I.V., Tu Z.;
RT "Genome analysis of a major urban malaria vector mosquito, Anopheles
RT stephensi.";
RL Genome Biol. 15:459-459(2014).
RN [2] {ECO:0000313|EnsemblMetazoa:ASTEI06660-PA}
RP IDENTIFICATION.
RC STRAIN=Indian {ECO:0000313|EnsemblMetazoa:ASTEI06660-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR AlphaFoldDB; A0A182YDX4; -.
DR STRING; 30069.A0A182YDX4; -.
DR EnsemblMetazoa; ASTEI06660-RA; ASTEI06660-PA; ASTEI06660.
DR VEuPathDB; VectorBase:ASTE007236; -.
DR VEuPathDB; VectorBase:ASTEI06660; -.
DR VEuPathDB; VectorBase:ASTEI20_042139; -.
DR OMA; SYLWMKE; -.
DR Proteomes; UP000076408; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533:SF290; AMINOPEPTIDASE; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000076408};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT DOMAIN 48..248
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 285..488
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 594..909
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 356
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 355
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 359
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 378
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 441
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1056 AA; 119959 MW; 669B7D288FF8B20B CRC64;
MKWHRCESAV RCAPVQTALR ILILSLSFIH SNGQSEPLNY RLPNETYPLR YNIELITRIH
DDTIGDDRFR FEGKVTIQLM ATDDRNAATD TITLNYRQID ITHVKLWYLI QNEWENVILD
DSTSFTVDPV REFLTIHSPQ PLNGSYFLEI KYNGTLREDN GGFYRSSYRT NDGKVQWLAT
TQFSPTDARH TFPCYDEPGI RAPIALRVIH GKNYSVLSNN VPIDVRESIM EGMVITTFPE
TPKMPSYLLG IIVSDFAEVS LPSYARQLAY ARPTALSTPA ANFILEAGFK VLAVLEDFLQ
TPYILRKLAH VAVPDFSPGA MENYGLITYK EENFLYNPQT SPMKQKKKIA TIVAHEIGHH
YFGNYVSPAW WSYLWMKEGF ARFFEYTAAQ MVFPEMEIGK MYTIDKTHNA FQTDSLSSSR
PMTYYVNTQT EISNIFDDIA YDKGGAVMLM FYHAFGQEPF RNALIKYIQD NALQAGTPAK
FAEAMQSTMF DTLPPDSLPF NASALLASWT DQAGYPILYI SRSGADCALR IEQQRYLLKS
NETSSSSAVQ WMIPYNFATA KNPSFQDTTY SGWITERFHT IKPTDDLSWT CDDWIVFNKQ
QTGYYRVNYD SDLWQLITTA LLQNSSAFHE NNRAQVIDDA MNNGRSGRLQ YAVPLQLLRY
LSKETDYVPW AAVDRNLALL NVLMRGTNKY DLWHKYCLEF VEPTYTLMGM TTLDQDTLTQ
RMTREIVVSW ACKVGSASCL NNTAFIVREI AENRLKDADP DLREPIFCNG LRNASAAVFD
SIWQRMQASQ DQAYRSELIR SLGCVESEPL VNQYLDSTIS TNSSNYYNQE RERVFMAVYR
NGEFGLQLAM KFLLENMDQV NQLYNKGNFG GRAISSIVRN MAQQITNEDM HTQLQDLMEK
LLEKGFLRSA DMLQALEYSS ENLMWIQERG AQIAAWLDEQ YPDPPPTTTT ISTTTTTTTP
ATTSTVTVTP TSTLSSAKSS TSDAISTSSI SITTISSLPT TISTKYDIIS NVSTSPMDSI
TSTENYDDNG ASHIQHDVFY STLLVVVSVL LIMFTQ
//
