ID A0A192H0H6_9LACO Unreviewed; 661 AA.
AC A0A192H0H6;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE SubName: Full=N-acetylglucosamine-specific PTS transporter subunit IIBC {ECO:0000313|EMBL:MDA5387182.1};
DE EC=2.7.1.193 {ECO:0000313|EMBL:MDA5387182.1};
DE SubName: Full=PTS N-acetylglucosamine transporter subunit IIABC {ECO:0000313|EMBL:ANK61783.1};
GN Name=nagE {ECO:0000313|EMBL:MDA5387182.1};
GN ORFNames=AYR53_02770 {ECO:0000313|EMBL:ANK61783.1}, OUQ91_002399
GN {ECO:0000313|EMBL:MDA5387182.1};
OS Loigolactobacillus backii.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Loigolactobacillus.
OX NCBI_TaxID=375175 {ECO:0000313|EMBL:ANK61783.1, ECO:0000313|Proteomes:UP000078582};
RN [1] {ECO:0000313|EMBL:ANK61783.1, ECO:0000313|Proteomes:UP000078582}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TMW 1.1989 {ECO:0000313|EMBL:ANK61783.1,
RC ECO:0000313|Proteomes:UP000078582};
RA Behr J., Geissler A.J., Vogel R.F.;
RT "Pediococcus and Lactobacillus from brewery environment - whole genome
RT sequencing and assembly.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MDA5387182.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KKP 3565 {ECO:0000313|EMBL:MDA5387182.1};
RG Lg backii sequencing group;
RA Kiousi D.E., Bucka Kolendo J.;
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CP014873; ANK61783.1; -; Genomic_DNA.
DR EMBL; JAPTYS010000007; MDA5387182.1; -; Genomic_DNA.
DR RefSeq; WP_068279492.1; NZ_JAPTYT010000007.1.
DR AlphaFoldDB; A0A192H0H6; -.
DR STRING; 375175.AYR53_02770; -.
DR GeneID; 42981160; -.
DR OrthoDB; 9764327at2; -.
DR Proteomes; UP000078582; Chromosome.
DR Proteomes; UP001150357; Unassembled WGS sequence.
DR GO; GO:0019866; C:organelle inner membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015572; F:N-acetylglucosamine transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00212; PTS_IIB_glc; 1.
DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1.
DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR036878; Glu_permease_IIB.
DR InterPro; IPR018113; PTrfase_EIIB_Cys.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR001996; PTS_IIB_1.
DR InterPro; IPR010974; PTS_IIBC_nag.
DR NCBIfam; TIGR00826; EIIB_glc; 1.
DR NCBIfam; TIGR00830; PTBA; 1.
DR NCBIfam; TIGR01998; PTS-II-BC-nag; 1.
DR PANTHER; PTHR30009; CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT; 1.
DR PANTHER; PTHR30009:SF4; PTS SYSTEM N-ACETYLGLUCOSAMINE-SPECIFIC EIICBA COMPONENT; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF51261; Duplicated hybrid motif; 1.
DR SUPFAM; SSF55604; Glucose permease domain IIB; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000078582};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:MDA5387182.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 71..88
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 108..129
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 175..197
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 209..228
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 297..319
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 350..369
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1..381
FT /note="PTS EIIC type-1"
FT /evidence="ECO:0000259|PROSITE:PS51103"
FT DOMAIN 404..486
FT /note="PTS EIIB type-1"
FT /evidence="ECO:0000259|PROSITE:PS51098"
FT DOMAIN 529..633
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000259|PROSITE:PS51093"
FT ACT_SITE 426
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421"
SQ SEQUENCE 661 AA; 70678 MW; CD95DD7ED6425769 CRC64;
MKAYLQRMGR SLQLPVAVLP AAALLVGIGH WLPQKWAAAQ FLQSGGNAIL TNLALLFAGG
LALGMSKDKD GASALAGLVA FIVPVYVLDP AQVSTLLGIK LSAVDPAFAA IKGNVLIGII
AGLVAAALYN RFHETKLPMA LSFFSGKRLV PILASLVMLV VSLVLLFVWP PLYEALVSFA
KFIVGLGAVG AGLYGFFNRL LIPTGLHQAL NSVFWFNVAG INDIGKFWAG EGVKGVTGMY
QAGFFPVMMF GLPAGAYAIY KNARPERKRE TASLMLAGAF ASFFTGVTEP LEFSFMFVAW
PLYVIHAVFT GISLAFAAFM HWTAGFTFSA GLVDFVLSSH LPIANMPYML LVQGLVMAVI
YYFGFDFAIK KFNLMTPGRE PVEETTDADT DIEVATDSSD DKYTVTAKKV YAAIGGKDNI
TDIENCTTRL RLQLKDTDKV NKAAIRGAGA VGMNVLDKTN IQIVIGTEVQ FVADALTKLF
ETGAPISNVA NSPAAPAKPI VDPNINSGAV DTFYSVAQGQ LLDIEQVSDP TFAQKMLGDG
YAIEPVNGEV VSPIDGEILT IFPTKHAIGL KTTDGLEVLV HMGLDTVELK GAPFDIKVKQ
GQKVKHGDVL ANADLQAIKD AGKKSTMIVI VTNMPNVAYM KFAELDRSIA ANNEVVKVTV
K
//
