ID A0A193KE90_9VIBR Unreviewed; 157 AA.
AC A0A193KE90;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=Transcription elongation factor GreA {ECO:0000256|ARBA:ARBA00013729, ECO:0000256|HAMAP-Rule:MF_00105};
DE AltName: Full=Transcript cleavage factor GreA {ECO:0000256|HAMAP-Rule:MF_00105};
GN Name=greA {ECO:0000256|HAMAP-Rule:MF_00105};
GN ORFNames=A6E01_10375 {ECO:0000313|EMBL:ANO33609.1}, BCU06_01980
GN {ECO:0000313|EMBL:PMK32169.1};
OS Vibrio breoganii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=553239 {ECO:0000313|EMBL:ANO33609.1, ECO:0000313|Proteomes:UP000092018};
RN [1] {ECO:0000313|EMBL:ANO33609.1, ECO:0000313|Proteomes:UP000092018}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FF50 {ECO:0000313|EMBL:ANO33609.1,
RC ECO:0000313|Proteomes:UP000092018};
RA Hehemann J.-H., Arevalo P., Datta M.S., Yu X., Corzett C., Henschel A.,
RA Preheim S.P., Timberlake S., Alm E.J., Polz M.F.;
RT "Adaptive Radiation by Waves of Gene Transfer Leads to Fine-Scale Resource
RT Partitioning in Marine Microbes.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000235835}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10N.261.54.C7 {ECO:0000313|Proteomes:UP000235835};
RA Kauffman K., Hussain F., Yang J., Arevalo P., Brown J., Cutler M.,
RA Kelly L., Polz M.F.;
RT "Nontailed viruses are major unrecognized killers of bacteria in the
RT ocean.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:PMK32169.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=10N.261.54.C7 {ECO:0000313|EMBL:PMK32169.1};
RA Kauffman K., Arevalo P., Polz M.F.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:PMK32169.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=10N.261.54.C7 {ECO:0000313|EMBL:PMK32169.1};
RX PubMed=29364876; DOI=.1038/nature25474;
RA Kauffman K.M., Hussain F.A., Yang J., Arevalo P., Brown J.M., Chang W.K.,
RA VanInsberghe D., Elsherbini J., Sharma R.S., Cutler M.B., Kelly L.,
RA Polz M.F.;
RT "A major lineage of non-tailed dsDNA viruses as unrecognized killers of
RT marine bacteria.";
RL Nature 554:118-122(2018).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC {ECO:0000256|ARBA:ARBA00024916, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family.
CC {ECO:0000256|ARBA:ARBA00008213, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
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DR EMBL; CP016177; ANO33609.1; -; Genomic_DNA.
DR EMBL; MCXF01000025; PMK32169.1; -; Genomic_DNA.
DR RefSeq; WP_017028361.1; NZ_SYVT01000035.1.
DR AlphaFoldDB; A0A193KE90; -.
DR STRING; 553239.A6E01_10375; -.
DR GeneID; 77304928; -.
DR KEGG; vbr:A6E01_10375; -.
DR eggNOG; COG0782; Bacteria.
DR OrthoDB; 9808774at2; -.
DR Proteomes; UP000092018; Chromosome 1.
DR Proteomes; UP000235835; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR NCBIfam; TIGR01462; greA; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF4; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00105}; Elongation factor {ECO:0000313|EMBL:ANO33609.1};
KW Protein biosynthesis {ECO:0000313|EMBL:ANO33609.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00105};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00105}.
FT DOMAIN 4..73
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 82..156
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
SQ SEQUENCE 157 AA; 17573 MW; 197D28FF340CB91A CRC64;
MEKVPMTVRG EQQLKTELER LLKLRPLIST AIAEARELGD LKENAEYHAA REEQGICEAQ
IRDIEYKLSV AQVIDVTKMD NSGKVIFGTT VTLIDVDTEE EFKYQIVGED EAEIKLGRIS
VSSPIARGLI GKMEGDEVTI TTPGGVKDYE IDRVEYL
//
