ID A0A194PM72_PAPXU Unreviewed; 528 AA.
AC A0A194PM72;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 22-FEB-2023, entry version 18.
DE RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN ORFNames=RR46_05788 {ECO:0000313|EMBL:KPI94536.1};
OS Papilio xuthus (Asian swallowtail butterfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Papilionoidea;
OC Papilionidae; Papilioninae; Papilio.
OX NCBI_TaxID=66420 {ECO:0000313|EMBL:KPI94536.1, ECO:0000313|Proteomes:UP000053268};
RN [1] {ECO:0000313|EMBL:KPI94536.1, ECO:0000313|Proteomes:UP000053268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ya'a_city_454_Px {ECO:0000313|EMBL:KPI94536.1};
RC TISSUE=Whole body {ECO:0000313|EMBL:KPI94536.1};
RX PubMed=26354079; DOI=10.1038/ncomms9212;
RA Li X., Fan D., Zhang W., Liu G., Zhang L., Zhao L., Fang X., Chen L.,
RA Dong Y., Chen Y., Ding Y., Zhao R., Feng M., Zhu Y., Feng Y., Jiang X.,
RA Zhu D., Xiang H., Feng X., Li S., Wang J., Zhang G., Kronforst M.R.,
RA Wang W.;
RT "Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies.";
RL Nat. Commun. 6:8212-8212(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC Evidence={ECO:0000256|ARBA:ARBA00001231,
CC ECO:0000256|PIRNR:PIRNR001093};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC {ECO:0000256|ARBA:ARBA00006285, ECO:0000256|PIRNR:PIRNR001093}.
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DR EMBL; KQ459598; KPI94536.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A194PM72; -.
DR STRING; 66420.A0A194PM72; -.
DR Proteomes; UP000053268; Unassembled WGS sequence.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd06562; GH20_HexA_HexB-like; 1.
DR Gene3D; 3.30.379.10; Chitobiase/beta-hexosaminidase domain 2-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR029018; Hex-like_dom2.
DR InterPro; IPR029019; HEX_eukaryotic_N.
DR PANTHER; PTHR22600; BETA-HEXOSAMINIDASE; 1.
DR PANTHER; PTHR22600:SF21; BETA-HEXOSAMINIDASE A; 1.
DR Pfam; PF00728; Glyco_hydro_20; 1.
DR Pfam; PF14845; Glycohydro_20b2; 1.
DR PIRSF; PIRSF001093; B-hxosamndse_ab_euk; 1.
DR PRINTS; PR00738; GLHYDRLASE20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF55545; beta-N-acetylhexosaminidase-like domain; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR001093-2};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR001093};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001093};
KW Reference proteome {ECO:0000313|Proteomes:UP000053268}.
FT DOMAIN 17..143
FT /note="Beta-hexosaminidase eukaryotic type N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14845"
FT DOMAIN 167..480
FT /note="Glycoside hydrolase family 20 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00728"
FT ACT_SITE 321
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-1"
FT DISULFID 47..104
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
FT DISULFID 276..326
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
FT DISULFID 508..526
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
SQ SEQUENCE 528 AA; 60714 MW; F0DE2EC9CF22FD3F CRC64;
MSIVNPGPKY PPSKGEVWPK PQNEITELTY YTFDPSNFKV KVTNNTCDIL TSAIERYSYI
VESRKPLRDQ RPVSVTERRQ HAREAQTLVL GVLQELQVQL TEPCEKYPYL EMDEKYRLNV
SAVSVLTSAS VWGILRGLET FAQKFYISHD MNELRINTSV VVDFPAYAHR GLLLDTGRHY
ISVPNILLTL DAMTINKMNV FHWHIVDDQS FPYQSEKFPD LSLYGAYHSS MVYTKDDIAR
IVEYARLRGI RVLPEFDVPG HTRSWGNAYP NILTACYRGN EVVGLGPMNP IRNITYKMIR
DLFHEVQAWF PDKYMHIGGD EVEMLCWRSN PEIREYMQEN GLKVEELHAL FMQNTIPLLA
DGSKIVVWQE VFDEDVPLTN DTLVQVWKSG WIDELVKVLE AGHRVLFSSA WYLDHLQNEW
PSLYMVDPRR IVRDATGSDT LPAGVIGGEA CMWGEMVDDR NVISRVWPRA SAVAERLWSA
PTVGQRRRYS SASLPHSEVY RRMEEHVCRM NRRGISAQPA SGPGYCLV
//
