ID A0A196N3L0_9SPHN Unreviewed; 631 AA.
AC A0A196N3L0;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|RuleBase:RU361168};
DE EC=3.2.1.22 {ECO:0000256|RuleBase:RU361168};
DE AltName: Full=Melibiase {ECO:0000256|RuleBase:RU361168};
GN ORFNames=A7X12_22325 {ECO:0000313|EMBL:OAN62232.1};
OS Sphingomonas sp. TDK1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=453247 {ECO:0000313|EMBL:OAN62232.1, ECO:0000313|Proteomes:UP000078445};
RN [1] {ECO:0000313|EMBL:OAN62232.1, ECO:0000313|Proteomes:UP000078445}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TDK1 {ECO:0000313|EMBL:OAN62232.1,
RC ECO:0000313|Proteomes:UP000078445};
RA Takahashi S.;
RT "Draft Genome Sequences of Sphingobium sp. strain TCM1 and Sphingomonas sp.
RT strain TDK1, Haloalkyl Organophosphorus Flame Retardant-Degrading
RT Bacteria.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|RuleBase:RU361168};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 27 family.
CC {ECO:0000256|ARBA:ARBA00009743, ECO:0000256|RuleBase:RU361168}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAN62232.1}.
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DR EMBL; LXVY01000027; OAN62232.1; -; Genomic_DNA.
DR RefSeq; WP_064312330.1; NZ_LXVY01000027.1.
DR AlphaFoldDB; A0A196N3L0; -.
DR STRING; 453247.A7X12_22325; -.
DR OrthoDB; 9807519at2; -.
DR Proteomes; UP000078445; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd14792; GH27; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.120.1060; NPCBM/NEW2 domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR013222; Glyco_hyd_98_carb-bd.
DR InterPro; IPR002241; Glyco_hydro_27.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR041233; Melibiase_C.
DR InterPro; IPR038637; NPCBM_sf.
DR PANTHER; PTHR11452:SF80; ALPHA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR11452; ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE; 1.
DR Pfam; PF16499; Melibiase_2; 2.
DR Pfam; PF17801; Melibiase_C; 1.
DR Pfam; PF08305; NPCBM; 1.
DR PRINTS; PR00740; GLHYDRLASE27.
DR SMART; SM00776; NPCBM; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|RuleBase:RU361168};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361168};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361168};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..631
FT /note="Alpha-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5008272610"
FT DOMAIN 489..631
FT /note="Glycosyl hydrolase family 98 putative carbohydrate-
FT binding module"
FT /evidence="ECO:0000259|SMART:SM00776"
SQ SEQUENCE 631 AA; 67937 MW; AA9C2E4E024AE7EA CRC64;
MAIKTLAGIS ALLLASASVQ AAPRDPLAPT GRWSANTDGR AATPPMGWNS WNAFATDVDE
EKVLASAQKI VDTGLAAKGY RYVNLDEGWW DHRRTDGRML VRADKFPSAR TADGATSFKP
FTDRLHAMGL KAGIYTDLGR NTCAQAYGPN EPNLPRGSVP EREVGLYGHI DQDIALYFGA
WGFDYIKIDG CGLRAYGEDS PLVRAGRYRP LAPILDLDSV NRSNIPAVRA MFAQVKAALV
RADPDQDYLL SLCIWGSADV RSWAKDVGNI SRTSDDITPH WTRLLTNFDT AARRPLYAHP
GSWNDPDMLF IGKGEFDADH LTEARSHFAL WAMVSAPLLI GADLRTTPQA LMDIFGNAAL
ISVDQDPAGN QAVLAYDSDD FQILVKTLAD GDKAVALFNR TAAPMEAILT AQHLKLRDDA
AIALTDLWSG ASSSFRKEAK FKLAPHETLV FRARGPRLLT DGLYLSEMPG SVNPAADGIT
APMADPTIHR AVVSWSGTRG AGQRPLYAGW GGAQADATPY GRELAVAGKH FTAGLGVLAN
SRLEVRNAGY RQLSVSVGVD DSATDAAHAV TFQIYGDRRL LATAAPVRRG APAKPLSVNV
AGVKLVELVA RSAGVVNEQL PIVWGDAAFH R
//
