ID A0A196SE17_BLAHN Unreviewed; 1757 AA.
AC A0A196SE17;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=AV274_4040 {ECO:0000313|EMBL:OAO14239.1};
OS Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII).
OC Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Opalinata; Blastocystidae;
OC Blastocystis.
OX NCBI_TaxID=478820 {ECO:0000313|EMBL:OAO14239.1, ECO:0000313|Proteomes:UP000078348};
RN [1] {ECO:0000313|EMBL:OAO14239.1, ECO:0000313|Proteomes:UP000078348}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50177 / NandII {ECO:0000313|Proteomes:UP000078348};
RA Gentekaki E., Curtis B., Stairs C., Eme L., Herman E., Klimes V.,
RA Arias M.C., Elias M., Hilliou F., Klute M., Malik S.-B., Pightling A.,
RA Rachubinski R., Salas D., Schlacht A., Suga H., Archibald J., Ball S.G.,
RA Clark G., Dacks J., Van Der Giezen M., Tsaousis A., Roger A.;
RT "Nuclear genome of Blastocystis sp. subtype 1 NandII.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAO14239.1}.
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DR EMBL; LXWW01000272; OAO14239.1; -; Genomic_DNA.
DR STRING; 478820.A0A196SE17; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000078348; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000078348};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 331..673
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 386..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1449..1527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..406
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1460..1474
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1475..1491
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1492..1515
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1757 AA; 198407 MW; F7D1474DB2DC8C62 CRC64;
MQSIISAPLT HEITRTHFGY YMPDEIKKLS VKKITNPVAF DGDGKPLPDG LYDPALGPVD
FHVKCPTCYR TFDDCPGHLG HIELELPIYN PMLFTLLIKI LRCTCQNCHR FKMNKYMIKR
YEIHKKTEFV EELDQLLHSQ KRNEDFEEKF DAVIAKYEAI IRSSKRLPKH EVVYTHTRAI
YNGLIREFLA AMPRICENCG AASPSIVRDG FLKLFEVPIK SKSKQFATNA MPTKSALDFL
TVSQLAKMEG VEEKEPEFAE AEMEEEEAPA LADDAAAKMK FLPPLEAEVR MRLLYEANHD
IINLIWYPQL TEAGAILNPT SEPEHANDGY KIFFVRTILV TPSRFRPPQH LHGESYEHPQ
NVFLSQILKD NYNLLKYGAR EQVEAEVEGS SASADKTDKT GKANKASAKD RAMGYTFENA
LHDWVDMQEA YNSLVDSAKS RNNKNSSGIR QLLEHKEGLF RTNLMGKRVN FAARSVIAPD
PYIDTDEVGV PLQFARELCY PEPVTANNVK MLRELVVNGR DAYPGACYLE NDKGFFIDLK
YKNRGQREAL AKMLMTKDAS GNVRMKRVWR NLLEGDILLV NRQPTLHKAS IMALRARILK
SKRQTIRMHY SNCNTFNADF DGDEINLHFP QGELARSEAY NIVCAKHQYP SARDGSPLRG
LIQDHVASGF LLTKRDTFLN HQEFDQLVFT AISGFDGRAR ERVVKTVPPC ILKPVTLWTG
KQVVSSLLIN LVGDLPRMNY EGNAQVADKN WGDGKIPAGS PLGEQQVLIE ECELLRGVLD
KSTFGAKNYS LVHAIHELYG VDLCSKFLSC FGRVVTSYFQ KRGFTCSMED LVLVDDAEKK
RRALIDGALE KAVAGLERYM SLEEDKGLTL EQRRTAVSKE LRKRFINSRG RVGPEIDNVM
KSIVSPISSE IFKVCLPHGL YVPFPHNAFS CMIISGAKGS NVNQTQVSCA LGQQELEGRR
VPMMSSGKTL PSFRPYDPMP RAGGWVSDRF LTGIRPQEYY FHAMAGREGL VDTAVKTSRS
GYLQRCVIKA MEDLSVQYDY SVRNSDGEVI QVLYGDDGVD VSKVKYLTGK PAQFDFLAKN
STILVYKYGL SEHFFQDTGL NSEEAAVKHA EVQYAREELA KHAEALSKKS VIEARRKLGK
GEWAVGNVSN EFVPAVITKV HKKDGSYDVV YSDTQEKEKH VSRRVFDSSE GIEIELIRNR
VPDPVNWQLN ICRNIGAVNE KYQKAIDSYL DTHSATGSVD KGGMEVLFWM KYLRSLVDPG
ESVGVIAGQS IGEPATQMTL NTFHLAGHGA VNVTLGIPRL REVIMTGSRT ILTPMMTIPL
IPAVKKASAE KLMKKLHRIG LNELLDPECY FRVVDYLTNT THGLKYTPYI RKYELHVNLL
PTEVIRKVYN LEMDGVLGVI MNEFLPLLGS LIAKDMKRSA RSVNIGLDIE KIVSGKDLSN
LKKTKLKKSE EKEVRGMADS EDDAEEDEEE VADVDGADAG RLNKAARVEY EEEEKESEED
EEKMETEEKE KEEESKEEME VEKKSVTHDK VPEGFTVLEA PFKVMKLKFF RRVMYNEATN
KLRVEFEFPP SVPKLLLVEL AEKAAQTVLV NSIQHINRCI VMDKDTDEDA NHTLQTEGVN
FPVVWMYQHL VDVNKVYSND IWQILETYGV EAARTAIITE IERVFDVYSI SIDPRHLSLI
ADYMTQDGSY RPMNRGGMEN MESPFQQMSF ETTTHFLNNA VLYGYSDSMK TPSACISTGK
VMKAGTGSFQ IVQPLNV
//
