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Database: UniProt
Entry: A0A197JGZ7_9FUNG
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ID   A0A197JGZ7_9FUNG        Unreviewed;       847 AA.
AC   A0A197JGZ7;
DT   05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2016, sequence version 1.
DT   03-JUL-2019, entry version 20.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   ORFNames=K457DRAFT_141950 {ECO:0000313|EMBL:OAQ24457.1};
OS   Mortierella elongata AG-77.
OC   Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota;
OC   Mortierellomycotina; Mortierellomycetes; Mortierellales;
OC   Mortierellaceae; Mortierella.
OX   NCBI_TaxID=1314771 {ECO:0000313|EMBL:OAQ24457.1, ECO:0000313|Proteomes:UP000078512};
RN   [1] {ECO:0000313|EMBL:OAQ24457.1, ECO:0000313|Proteomes:UP000078512}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AG-77 {ECO:0000313|EMBL:OAQ24457.1,
RC   ECO:0000313|Proteomes:UP000078512};
RG   DOE Joint Genome Institute;
RA   Uehling J., Gryganskyi A., Hameed K., Tschaplinski T., Misztal P.,
RA   Wu S., Desiro A., Vande Pol N., Du Z.-Y., Zienkiewicz A.,
RA   Zienkiewicz K., Morin E., Tisserant E., Splivallo R., Hainaut M.,
RA   Henrissat B., Ohm R., Kuo A., Yan J., Lipzen A., Nolan M., Labutti K.,
RA   Barry K., Goldstein A., Labbe J., Schadt C., Tuskan G., Grigoriev I.,
RA   Martin F., Vilgalys R., Bonito G.;
RT   "Genome sequencing reveals origins of a unique bacterial endosymbiosis
RT   in the earliest lineages of terrestrial Fungi.";
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
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DR   EMBL; KV442094; OAQ24457.1; -; Genomic_DNA.
DR   EnsemblFungi; OAQ24457; OAQ24457; K457DRAFT_141950.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000078512; Unassembled WGS sequence.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000078512};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000078512}.
FT   DOMAIN      402    847       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    593    593       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       407    407       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       409    409       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       490    490       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       490    490       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       519    519       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       545    545       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       633    633       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     492    492       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     490    490       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   847 AA;  90982 MW;  045C9550A08835E9 CRC64;
     MKLVPRELDK LVLHQVGFLA QKRLARGLRL NHTETVALIA SQLAEFIRDG RHSVADLMDL
     GKQLLGRRQV MPGVAETVHE VQIEGTFPDG TKLVTVHDPI SNESGNLELA LHGSFLPIPD
     VDRYFPPITE VEEATFVHPP GAIVTAPGTL TLNKGRKRFR LEITNTGDRP ILVGSHFHLI
     EANSALKMDR ALAYGHRLDI PAGSAIRFEP GEKNTVTIVS IAGNKKISGG NNLASGTVNP
     GKLSEIMANL VAKNFAHMEQ SPALNAKELP EYQIPREVYQ SFYGPTTGDR VRLGDTDLWI
     EIEKDFTHYG DECKFGGGKV LREGMGQANG VPDSEALDLV ITNCIILDYT GIYKADIGVK
     NGVISGIGKA GNPDVMEGVM PNMVVGVSTE VMAGEHQIVV AGGIDTHVHY ICPQLIEEAL
     TSGLTTLVGG GTGPNTGSNA TTCTPAAEFI KMMMQATDAS PINIGITGKG NTAHLNGIHD
     QVKAGVIGLK LHEDWGTTPA AIDNCLTACD MYDVQATIHT DTLNEAGFVQ STVGAIKGRT
     IHTYHTEGAG GGHAPDIITV CGQPNVLPSS TTPTLPFTKN TLDEHIDMLM VCHHLDKNLP
     EDVHFAESRI RGETIAAEGC LHDMGAISMI SSDAQAMGRI GEVVGRTWML ADRMKQVRGH
     IDDEDQSAFK TTADNFRVKR YVAKYTINPA VAHGISHLVG SIEIGKVADL VMYEPASFGS
     KPTHVLKSGV VTFSAMGLAN GSIPSTQPII LRPMFGAIGS AAAANSIIFM SQAAVELNRG
     KEYGLKKRVE AIRKCRDISK ADMRLNNVCP VVEVDPESFE VSVEGIMISD MVKPADSLPL
     AQSMYIF
//
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