ID A0A197KEJ5_9FUNG Unreviewed; 1071 AA.
AC A0A197KEJ5;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 13-SEP-2023, entry version 27.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=K457DRAFT_132115 {ECO:0000313|EMBL:OAQ36137.1};
OS Linnemannia elongata AG-77.
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mortierellomycotina;
OC Mortierellomycetes; Mortierellales; Mortierellaceae; Linnemannia.
OX NCBI_TaxID=1314771 {ECO:0000313|EMBL:OAQ36137.1, ECO:0000313|Proteomes:UP000078512};
RN [1] {ECO:0000313|EMBL:OAQ36137.1, ECO:0000313|Proteomes:UP000078512}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AG-77 {ECO:0000313|EMBL:OAQ36137.1,
RC ECO:0000313|Proteomes:UP000078512};
RG DOE Joint Genome Institute;
RA Uehling J., Gryganskyi A., Hameed K., Tschaplinski T., Misztal P., Wu S.,
RA Desiro A., Vande Pol N., Du Z.-Y., Zienkiewicz A., Zienkiewicz K.,
RA Morin E., Tisserant E., Splivallo R., Hainaut M., Henrissat B., Ohm R.,
RA Kuo A., Yan J., Lipzen A., Nolan M., Labutti K., Barry K., Goldstein A.,
RA Labbe J., Schadt C., Tuskan G., Grigoriev I., Martin F., Vilgalys R.,
RA Bonito G.;
RT "Genome sequencing reveals origins of a unique bacterial endosymbiosis in
RT the earliest lineages of terrestrial Fungi.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; KV442012; OAQ36137.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A197KEJ5; -.
DR STRING; 1314771.A0A197KEJ5; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000078512; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000078512}.
FT DOMAIN 16..640
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 694..849
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT COILED 1010..1037
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1071 AA; 123810 MW; 1592A6B297F912C3 CRC64;
MADGSAQFNF PKEEEKILEF WREIDAFKRS QELNKDKEPF AFFDGPPFAT GLPHYGHLLA
GTIKDIVTRF AHSTGHSVER RFGWDCHGLP VEYEVDKALG ITGKADVLAM GIDKYNEECR
SIVMRYAKEW RQTVERAGRW IDFDNDYKTL NVEFMESVWW VFKTLFDKGQ VYRGVRVMPF
STACSTPVSN FEAQQNYKDV SDPSVVVSFP LLNDPSVAFL AWTTTPWTLP SNLALCVNPT
HDFIKFLDEA SGNTYIMIEK RLSILYKDPS KAKIKILERM KGADLKGIQY VPLFEYFYKE
FEATGFRILN DEYVDDSAGT GVVHQAPAFG EDDYRVCINH GVITADGHIP CPVDESGKYF
DVITDFKGMH VKEADKLIQK HIKGMGRLIV QTQINHSYPF CWRSDTPLIY KAFPSWFVRV
QPIVDKMLKN NAQSRWVPGF VQEKRFANWI ANARDWNISR SRYWGTPIPI WATEDFSEMH
CIGSLAELEE LSGCGKLTDI HRHFIDHITF KSPKTGKTMR RVEDVFDCWF ESGSMPYAQQ
HYPFENKESF SKGFPADFIS EGLDQTRGWF YTLLVLSTHL FDTPPAKNQI VSGLVLASDG
KKMSKRLKNY PEANIILDAY GADALRLYLI NSPVVRAETL KFKEEGVKDV ITKVFLPWYN
AYKFFVNQTV LLKKEFNHDF MYNGALKKSD NVMDRWMLAS CQSLIKFVRE EMTNYRLYTV
VPRLLRLIEE LTNWYVRFNR RRLKGENGLE DATQAMNTLF EVLYTLVRVM SPFTPFLTEN
MYQTLKNFVP KDPKIVDDRS VHFLDFPEVK EEYFDDDIER AMSRMQAVIE LGRTTRERKT
ISLKTPLKEL VVIHPDPQYH ADVKSLESYI VDELNVRSLI VTSDEAQYGV KYTIEADFRS
LGAKLKKDAV KVKNALPKLS EEQIKDFIRN KAITIEGHNL GEEDLTVVRF FESSENSLET
NSDKDVLILL DVKRYPELEQ EGLAREIINR VQRLRKKAGL EPTDMIHMYY EFTQDLNNAL
EQVIKEQEET LVRALKRSLE PIATKKEADA VIIEEAQEVN GSTFNLTLVR D
//
