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Database: UniProt
Entry: A0A1A0MY64_9MYCO
LinkDB: A0A1A0MY64_9MYCO
Original site: A0A1A0MY64_9MYCO 
ID   A0A1A0MY64_9MYCO        Unreviewed;       496 AA.
AC   A0A1A0MY64;
DT   05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2016, sequence version 1.
DT   08-MAY-2019, entry version 27.
DE   RecName: Full=Urease {ECO:0000256|RuleBase:RU000510, ECO:0000256|SAAS:SAAS00325551};
DE            EC=3.5.1.5 {ECO:0000256|RuleBase:RU000510, ECO:0000256|SAAS:SAAS00325551};
DE   Flags: Fragment;
GN   ORFNames=A5662_23215 {ECO:0000313|EMBL:OBA90340.1};
OS   Mycobacteriaceae bacterium 1482268.1.
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae.
OX   NCBI_TaxID=1834080 {ECO:0000313|EMBL:OBA90340.1, ECO:0000313|Proteomes:UP000091934};
RN   [1] {ECO:0000313|EMBL:OBA90340.1, ECO:0000313|Proteomes:UP000091934}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1482268.1 {ECO:0000313|EMBL:OBA90340.1,
RC   ECO:0000313|Proteomes:UP000091934};
RA   Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL   Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|RuleBase:RU000510,
CC         ECO:0000256|SAAS:SAAS01119912};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRSR:PIRSR611612-51,
CC         ECO:0000256|RuleBase:RU000510};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRSR:PIRSR611612-51, ECO:0000256|RuleBase:RU000510};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|SAAS:SAAS00317636}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PROSITE-
CC       ProRule:PRU00700}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR611612-50}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases
CC       superfamily. Urease alpha subunit family.
CC       {ECO:0000256|RuleBase:RU004158}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:OBA90340.1}.
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DR   EMBL; LZSH01000441; OBA90340.1; -; Genomic_DNA.
DR   RefSeq; WP_067074710.1; NZ_LZSH01000441.1.
DR   EnsemblBacteria; OBA90340; OBA90340; A5662_23215.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000091934; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000091934};
KW   Cytoplasm {ECO:0000256|PROSITE-ProRule:PRU00700};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00700,
KW   ECO:0000256|RuleBase:RU000510, ECO:0000256|SAAS:SAAS00321417};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR611612-51,
KW   ECO:0000256|RuleBase:RU000510, ECO:0000256|SAAS:SAAS00321440};
KW   Nickel {ECO:0000256|PIRSR:PIRSR611612-51,
KW   ECO:0000256|RuleBase:RU000510, ECO:0000256|SAAS:SAAS00317628};
KW   Reference proteome {ECO:0000313|Proteomes:UP000091934}.
FT   DOMAIN      136    496       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    327    327       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       141    141       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       143    143       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       224    224       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       224    224       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       253    253       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       279    279       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       367    367       Nickel 1. {ECO:0000256|PIRSR:PIRSR611612-
FT                                51}.
FT   BINDING     226    226       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     224    224       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
FT   NON_TER     496    496       {ECO:0000313|EMBL:OBA90340.1}.
SQ   SEQUENCE   496 AA;  51462 MW;  4453A65F5C001237 CRC64;
     MGELSRARYA ALFGPTTGDR IRLADTDLFV EITEDRSGGP GLAGDEAVFG GGKVLRESMG
     QGRATRADGA PDTVITGAVI IDYWGIIKAD IGIRDGRIAA IGKAGNPDTM TGVHPDLIVG
     PSTEIIAGNG RIVTAGAIDC HVHLICPQIM EEALGGGITT IVAGGTGPAE GSKATTVTPG
     GWHLARILES LDGWPLNVAL LGKGNTVSTE AMWEQLRAGA AGFKLHEDWG TTPAAIDACL
     TVADASGVQV NIHTDTLNEM GFVENTLGAI AGRSIHAYHT EGAGGGHAPD IITVAAESHV
     LPSSTNPTRP HTVNTLDEHL DMLMVCHHLN PSVPEDLAFA ESRIRPSTIA AEDLLHDIGA
     ISMIGSDAQA MGRIGEVVLR TWQTAHVMKR RRGAADGDGA DGRGADNNRV RRYVAKYTIC
     PAVAHGLDAE IGSVEVGKLA DLVLWEPAFF GVRPHAVVKG GMIAWAAMGD ANASIPTPQP
     VLPRPMFGAA PAAAAA
//
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