ID A0A1A2B539_9MYCO Unreviewed; 1258 AA.
AC A0A1A2B539;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA gyrase subunit A {ECO:0000256|ARBA:ARBA00026190};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=A5753_12845 {ECO:0000313|EMBL:OBF63173.1};
OS Mycobacterium sp. 852002-51971_SCH5477799-a.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=1834106 {ECO:0000313|EMBL:OBF63173.1, ECO:0000313|Proteomes:UP000091887};
RN [1] {ECO:0000313|EMBL:OBF63173.1, ECO:0000313|Proteomes:UP000091887}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=852002-51971_SCH5477799-a {ECO:0000313|EMBL:OBF63173.1,
RC ECO:0000313|Proteomes:UP000091887};
RA Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. {ECO:0000256|ARBA:ARBA00008263}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBF63173.1}.
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DR EMBL; LZIF01000104; OBF63173.1; -; Genomic_DNA.
DR RefSeq; WP_067129582.1; NZ_LZIF01000104.1.
DR AlphaFoldDB; A0A1A2B539; -.
DR STRING; 1834106.A5753_12845; -.
DR OrthoDB; 9806486at2; -.
DR Proteomes; UP000091887; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 3.30.1360.40; -; 1.
DR Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 2.
DR Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR NCBIfam; TIGR01063; gyrA; 1.
DR NCBIfam; NF038098; GyrA_w_intein; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR Pfam; PF03989; DNA_gyraseA_C; 6.
DR Pfam; PF00521; DNA_topoisoIV; 2.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 2.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 256..396
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 528..551
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT COILED 869..896
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1258 AA; 139455 MW; B3E5B5D5ED3B29C9 CRC64;
MTDTTLPPGD DSVDRIQPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM
YDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP
GNDPPAAMRY CVTGDALVRL PLGQAVRIGG VVPGCRSNSD NVLDLKVLDR HGNPVLADRL
FHSGDHQTYT VRTAEGYEVT GTANHPLLCL VDVGGVPTLL WKLISEIKQD DHVVLQRNEP
VELGPADWQP TMEALLLGAF ISEGFVSEKR AGFNNLDRDY FATVVAAYDA VVGGQRYVSS
RRIASGSILH ELDIHNLTAL QQSRLAELSK QRSAQKVVPE WLWNAPAAVK RVFLGALFEG
DGSCSALPRN TIQVSYSTRS ERLAKDVQQM LLEFGVVSKR YRHAVGEYKV VITNRAQAEL
FARQIGFGGV KQDKLVGILS AMPPCAGQDT DHVPGLAQFI RKHCGSRWTD KDWLNRHNID
RLSCWRRDGE QILSRIADPD VRAVATDLTD GRFYYARVAA VTEAGVQPVY SLRVDTQDHA
FITNGFVSHN TEARLTPLAM EMLREIDEET VDFIPNYDGR VQEPTVLPSR FPNLLANGSG
GIAVGMATNI PPHNLRELAE AVYWCLDNYE ADEEATLTAV RERVKGPDFP THGLIVGSQG
IHDAYTTGRG SVRMRGVVEV EEDSRGRTSL VITELPYQVN HDNFITSIAE QVRDGKLAGI
SNIEDQSSDR VGLRIVVEIK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD
QMIRYYVAHQ LDVIVRRTTY RLRKANERAH ILRGLVKALD ALDEVIALIR ASETVEIARA
GLIELLDIDE IQAQAILDMQ LRRLAALERQ RIVDDLAKIE AEIADLEDIL AKPERQRRIV
HDELSEIVDK YGDERRTRII AADGDVNDED LIAREDVVVT ITETGYAKRT KTDLYRSQKR
GGKGVQGAGL KQDDIVRHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL
LAFQPEERIA QVIQIRGYED APYLVLATRN GLVKKTKLTD FDSNRSGGIV AVNLRDGDEL
VGAVLCSADE DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNADD YLLSLNVVRE
GTYLLVATSG GYAKRTAIEE YPVQGRGGKG VLTVMYDRRR GRLVGALIVD EDSELYAITS
GGGVIRTAAR QVRKAGRQTK GVRLMNLGEE NTLLAIARNA EENADEVVEQ IDGAQSDS
//
