ID A0A1A3N0M5_MYCAS Unreviewed; 4169 AA.
AC A0A1A3N0M5;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:OBK15336.1};
GN ORFNames=A5636_05970 {ECO:0000313|EMBL:OBK15336.1};
OS Mycobacterium asiaticum.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=1790 {ECO:0000313|EMBL:OBK15336.1, ECO:0000313|Proteomes:UP000093629};
RN [1] {ECO:0000313|EMBL:OBK15336.1, ECO:0000313|Proteomes:UP000093629}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1245139.5 {ECO:0000313|EMBL:OBK15336.1,
RC ECO:0000313|Proteomes:UP000093629};
RA Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBK15336.1}.
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DR EMBL; LZLQ01000086; OBK15336.1; -; Genomic_DNA.
DR RefSeq; WP_065159086.1; NZ_LZLQ01000086.1.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000093629; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd05195; enoyl_red; 2.
DR CDD; cd08956; KR_3_FAS_SDR_x; 2.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 6.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF13602; ADH_zinc_N_2; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF21089; PKS_DH_N; 2.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00826; PKS_DH; 2.
DR SMART; SM00829; PKS_ER; 2.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF50129; GroES-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000093629};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 34..455
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1979..2054
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2072..2493
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4010..4085
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT COILED 2..32
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 4169 AA; 434716 MW; 1BC0E025949BFB9B CRC64;
MADQLQHATE ALRKALVQVE RLKRTNRALL ERSSEPIAIV GMSCRFPGGV DSPDSLWQMV
ADGRDVIAEF PTDRGWDVGS LFDPDPDAPH KTYANTGGFV DGVADFDPAF FGIAPSEALA
MDPQHRMLLE LSWEALERAG IDPGGLRGSA TGVFAGIIAQ GYGMFAEEIE GYRLTGMTSS
VASGRVSYVL GLEGPAVSVD TACSSSLVAL HMAVQSLRTG ECDLALAGGA TVNATPTVFV
EFSRHRGLAP DGRCKAYAGA ADGVGWSEGG AMLVVERLSD AQRLGHPVLA VVRGSAVNQD
GASNGLTAPN GPSQQRVVRA ALANAGLSTA DVDVVEGHGT GTTLGDPIEA QALLATYGQD
RSQPLWLGSI KSNMGHTQAA AGIAGVIKMV QAMRHEVLPA TLHVDVPSPH VDWSAGSVEL
LAQARPWTTE SARNRRAAVS SFGISGTNAH VIIESVPAEE PRQSRPAPSA LPWVLSAKSA
SAVTAQAARL AEHLRARPEL DLADVAWSLA GRSTFEHRAV VVGGDREQLL AGLGELASDE
FGEVIRGAAR PAGKTVFVFP GQGSQWVGMG IELLDTAPVF ARQIEACAEA FAEFVDWSLT
DVLRAAPGAP ALDRVDVVQP VLFAVMVSLA ELWKSVGVRP DAVIGHSQGE IAAAYVAGAL
SLRDAAKVVT LRSKLLTGLA GPGGMVSIAC GIERARELLA PFGDRIDIAA VNGRSAVVVS
GEVAALDELI RLCTEHELRV RRIDVDYASH SAAVEAIRTE LGEVLAGIEP VSTRTAFYST
VTGSRLDTAA LDADYWYRNI RQTVQLDQAV RSACEHGYRV FIESSPHPAL IAGIEDTVTD
CLAGDAEPVV VPTLGREDGG LHRFLSSAAL AFVGGVAVDW RGMLDALSGG RLVELPTYAF
DKRRFWLSGE GAGADASGLG LTASEHALLR AVVELPASGG VVLTGRLSAG AQSWLADHAV
SGMAVFPGTG FVELAIRAGD EVGCSVVDEL TLRSPLMIPA SGSVAVQVVV GPAASESGQE
TEHEGGHRSV SIYSHADVDG ASAWVCHAEG TLSSGTVEPG CDLAVWPPAG AVAVDLTGGY
ERLAEHGYGY GPAFRGLTAL WSRGEELFAE VRLPEAAGGS AGFGVHPALL DAALHAAVLA
NPEAELALPF SWQGVSLHAA GASTVRARIA PAGNAALSIE LADGLGLPVL TVAAMVARPV
TEQRLRAALS GVGPDRLFEL AWSQATFNTA SGAEATPVHD IFNSVATEDD PVTATYDRAH
RALAAVQSWL AEHDSGVLVV ATRGAMALPG EDIQDLAGAA VWGLLRSAQT ENPGRLVLVD
SDAAVDDAAA ATALAVGEPQ ILMRNGDVYT ARVRASRAVD GILEPPGDGP WRLGLSSAGT
FENLRLEPVP NGDAPLQPGH VRVAMRAIAA NFRDIMITLG MFTHEALLGG EGAGVVVEVG
PGVTEFAVGD SVYGFFPDTS GTLVPGDIRL LLPMPSDWTY AEAAAISAVF TTAWFAFIDL
AAVKPGQRVL IHAAAGGVGM AATQLARHLG LEVFGTASKG KWDTLRAMGF DDDHISDSRS
LDFEDKFRVV TGGAGMDVVL DSLAGEFVDA SLRLVAPGGV FLEMGKTDIR DPGVIAQEYP
GVRYRAFDLF EPGRPRMHQY MLELAELFDA GVLRPLPVTT FDIRRAPAAL RYLSQARHIG
KVVMTIPAGA GNGLGHGTVL ITGGTGMAGS LLARHVVAQH GVRHLVLISR RGPEAPGAAE
LTAELEAAGT QVQVVACDAA DRPALAKVIA DIPVQRPLSA VIHAAGVLDD ATVTSLTPDR
VDAVLRAKVD AAWNLHELTR DLNVSAFVMF SSMAGLVGSS GQANYSAANC FLDALAAHRR
AHHLPAMSLG WGLWEQASAM TGGLDAAGRA RLGREGVLAL SSQEALELFD TALIVDEPFL
APARIDLAAL RAHAAVPPMF RDLVTGPGRR QVDESLAAAK SKSALAHRLH GLSEAEQHAV
LLDLVRSHIA TVLGNVAPEA IDPDKAFQEL GFDSLTAVEM RNRLKSATGL SLSPTLIFDY
PTPSGLASYM RTELAGMPQE IKQVPAVRAT GDDAIAIVGM SCRYPGGVGS PEDLWDMVLQ
GRDVLSDFPT DRGWDLAGLY NRDPDVPGTC YTRTGGFVDG VADFDPAFFG IAPSEALAMD
PQQRLYLELS WEALERAGIE PGALRGSATG VFAGVYTQGY GIGAAQTAEG FRLTGQSSSI
ASGRVAYVLG LEGPAVSVDT ACSSSLVALH MAVQSLRSGE CDLALAGGVT VNATPDIFVE
FSRMRGLSAD GRCKAFAGAA DGTGFAEGGG MLVVERLSDA QRLGHPVLAL VRGSAINQDG
ASNGLTAPNG PSQQRVVRAA LASAGLSAVD VDAVEGHGTG TTLGDPIEAQ ALLATYGQGR
SEPLWLGSIK SNMGHTQAAA GVAGVIKMVQ AMRHEVLPAT LHVDAPSPHV DWSTGSVSLL
TESRPWLAQS GIRRAGVSSF GISGTNAHVI IESVPAPASA EATGPRPAVL PWVISAKTPS
ALQSQAARLA EYVGAHGESD VTDVGWTLAA RSSFEHRAVV VGADRDQLLA GLAEVAGDEV
GEVIRGVAQP AAKTVFVFPG QGSQLLGMGM GLHAVYPVFA EAFNTVVAEL DRHLLRPLRE
VMWGHDENLL NTTEFAQPAL FAVEVALFRL LESWDVRPDF VMGHSVGELS AAHVAGVLSL
ENAAVLVAAR GRFMQRLDPG GAMIALQATE AEVRPLLSNQ VSIAAVNGPA SVVISGDAEE
VVSVAESLRA DGRRVHQLAV SHAFHSPLMD PMIDEFGAVA AGLPIGEPTI PIISNLTGQL
AGDDYGSADY WKRHVREAVR FSDSIRFAQA AGATRFLELG PSGGLTTSIE ESLPDVAVTT
VYALRKDRPE PTTLITALAQ AFVTGAQVNW RAVLGHANLI ELPTYAFERR RFWLSNDGGT
VDAAGLGLAA GEHPLLGAVV DLPSSGGVVL TGRLSAARHA WLRDHAVGGV VILPGAGFVE
LAIRAGDEVG CSTVEELTLA APLVLPESGS VAVQVVVDAA DESGTRALSV YSRAEAGSGW
VLHAEGAVSP AAVQAGADLS AWPPQGAAPV DVSGLYDRLA DDGYGYGPAF QGLTAMWRRG
EDLFAEVTLP AMAGLSPAGF GVHPVVLDAA LHAIIAGADA EPDRPEGVLV PFAWQGVSLH
AAGASAVRAR IARTGPSAVS VELADALGLP VLSVASMVAR PVTEQQLKAA LAGSGGDRLF
DVIWSPAPAV LTGGTPPYQV YESQPVAGDT VAAVYAMTHQ VLEVVQSWLR EHDSGVLVVA
TRGAVAGPGE AITDLAGAAV WGLVRSAQTE HPGRIVLVDT DVPIDEEAAA AVLAVGEPQV
LLRNGTTHIA RVRGSRAVRD LLVPPGDVPW RLGMSTAGTF ENLRMEPIPD TDTALAPRHV
RVAASAMAAN FRDVMIALGL YPDEDAAMGV EASGAVIETG AAASCFAIGD RVTGLFPEGT
GAAATTDERL LIKIPAGWTD TEAATAPVVF ATAYYALTAL ADIQPGQRLL VHAATGGVGM
AAVQLARHLG AEVFATASKG KWDTLRRLGF DDDHIADSRS VEFEDKFRAV AGDRGIDVVL
DSLAGEFVDA SLRLVAPGGV FLEMGKTDIR DQETVAQQHT GVRYRAFDLF EAGPDHIQRM
LTELTGLFEA GVLRPLPVTA FDVRRAPAAL RYLSQARHIG KVVMTVPPAL AAGTVAEVPS
GLGGGTVLIT GGTGMAGAAL ARHVVHRHRV ARLILVSRRG PDAPGAGELT AELTASGAEV
HVVACDAADR EALAKVLAGI PEQQPLTAVI HAAGTLDDAV VTSLTPDRVD AALRSKVDAA
WNLHELTSGL NLSAFVLFSS IAGLAGASGQ ANYAAGNSFL DALAVYRHAQ GLPAISLGWG
LWDQASNMTE GLAAADLARF ARDGIVAMSS DEALELLDTA LIVDEPFLLP AHIDLVALRA
KFDAGTLPPM FVDLINAPAR RQVDDSLAAA KSKSALLQRL EGLPEDEQHA ILLDLVRSHI
ATVLGNTSPE AIDPDRAFQE LGFDSLTAVE MRNRLKAATG LPLSPTLIFD YPNSAALAGY
FHQELVGENT PATQPAAPGE EELRRVVASI PVKRLRQAGV LDVLLALANE KDGGLNGTNG
TPHSGTDAKD IADMDLDDLV NAAFLNDDE
//
