ID A0A1A5WRL4_9BACL Unreviewed; 367 AA.
AC A0A1A5WRL4;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369};
GN ORFNames=A6764_20635 {ECO:0000313|EMBL:OBR43857.1}, A6764_21585
GN {ECO:0000313|EMBL:UYZ13318.1};
OS Brevibacillus sp. WF146.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX NCBI_TaxID=319501 {ECO:0000313|EMBL:OBR43857.1};
RN [1] {ECO:0000313|EMBL:OBR43857.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WF146 {ECO:0000313|EMBL:OBR43857.1};
RA Evans L.H., Alamgir A., Owens N., Weber N.D., Virtaneva K., Barbian K.,
RA Babar A., Rosenke K.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:UYZ13318.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WF146 {ECO:0000313|EMBL:UYZ13318.1};
RA Tang B., Tang X.-F., Zhu F., Li Y.;
RT "Complete genome sequence of Brevibacillus thermoruber WF146.";
RL Submitted (OCT-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC {ECO:0000256|ARBA:ARBA00005163}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
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DR EMBL; LXGJ01000326; OBR43857.1; -; Genomic_DNA.
DR EMBL; CP109943; UYZ13318.1; -; Genomic_DNA.
DR RefSeq; WP_065068276.1; NZ_LXGJ01000326.1.
DR AlphaFoldDB; A0A1A5WRL4; -.
DR UniPathway; UPA00223; -.
DR Proteomes; UP000093585; Chromosome.
DR GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06109; BsCS-I_like; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR11739:SF4; CITRATE SYNTHASE, PEROXISOMAL; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000093585};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369}.
FT ACT_SITE 257
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 312
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 367 AA; 40324 MW; FC9F6F7C201F404B CRC64;
MSQHHTLAIG LEGIAVAETD LSLVDGLNGL LVYRGHWARD LAINHTFEEV AHLLWYGHLP
NEQELDELKD KLKANRELPA HVKSILDLIP ADVDMMSVLR TGVSALGSAD YGWPPTLDQV
ISLTAKMPTI VAYRYARLCG REPLDPNPAL DHTANYLYML KGQEPNPAHV RALDAYLILT
QEHGMNASTF SGRVVSSTQS DLISSITAAI GALKGPLHGG APSEVTEMIE AIGTKENAEP
WLRAKLEKGE RLMGFGHRVY KTVDPRATAL RVVASQLSGD DPWFDLATHV EQVGLKLLEE
YKPGRKLNTN VEFFAAAVMR AVGLDDSLFT PTFAVSRVVG WSAHILEQAK NNRIIRPQSI
YTGPMPE
//
