ID A0A1A5XMC5_9BURK Unreviewed; 514 AA.
AC A0A1A5XMC5;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=Hopanoid C-3 methylase HpnR {ECO:0000313|EMBL:OBR54651.1};
GN ORFNames=A6456_35260 {ECO:0000313|EMBL:OBR54651.1}, C7399_113175
GN {ECO:0000313|EMBL:PZW79077.1};
OS Paraburkholderia tropica.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Paraburkholderia.
OX NCBI_TaxID=92647 {ECO:0000313|EMBL:OBR54651.1, ECO:0000313|Proteomes:UP000093802};
RN [1] {ECO:0000313|Proteomes:UP000093802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P-31 {ECO:0000313|Proteomes:UP000093802};
RA Kaur C., Selvakumar G., Ganeshamurthy A.N.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OBR54651.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P-31 {ECO:0000313|EMBL:OBR54651.1};
RA Evans L.H., Alamgir A., Owens N., Weber N.D., Virtaneva K., Barbian K.,
RA Babar A., Rosenke K.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:PZW79077.1, ECO:0000313|Proteomes:UP000248793}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SIr-6529 {ECO:0000313|EMBL:PZW79077.1,
RC ECO:0000313|Proteomes:UP000248793};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-V): Genome sequencing
RT to study the core and pangenomes of soil and plant-associated
RT prokaryotes.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBR54651.1}.
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DR EMBL; LXGI01000011; OBR54651.1; -; Genomic_DNA.
DR EMBL; QKZC01000013; PZW79077.1; -; Genomic_DNA.
DR RefSeq; WP_065057557.1; NZ_QKZC01000013.1.
DR AlphaFoldDB; A0A1A5XMC5; -.
DR STRING; 92647.SAMN05216550_11450; -.
DR GeneID; 61303947; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000093802; Unassembled WGS sequence.
DR Proteomes; UP000248793; Unassembled WGS sequence.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR027564; HpnR_B12_rSAM.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04367; HpnR_B12_rSAM; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDF00565; hopanoid_C3-methyltransferase; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methyltransferase {ECO:0000313|EMBL:OBR54651.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000093802};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000313|EMBL:OBR54651.1}.
FT DOMAIN 8..141
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 181..395
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 514 AA; 57974 MW; DAA157D85701905E CRC64;
MRLLAVHPSG LMYTRVFLRL EPLGLESVAG TARDAGHEVQ LLDLQVETHR DLMRRVRNWR
PEALCFSGNY LANIPEIVDL AKAVKAVLPS CFVFVGGHSV SFTAHDLLRH AEGAIDCILR
GEGEASINDL LAVVAQGGDL LSVAGVVTRD GSGPPPRFVE HLDPVRPARD LLRHRRKYFI
GTLDPCASIE FARGCPWDCT FCSAWTFYGR SYRTRSPEVV VEELAALREP GVFIVDDVAF
VHAEHGMAIA DAIRQRGIKK RYYLETRGDV LLRNKDVFRA WESLGLKYMF LGLEAIDEAG
LKAFRKRISL DRNFEALEFA RSLGITVAIN LIADPDWDHD RFETIRQWCL EIPEIVNISV
NTPYPGTENW QQESRPLTSL DYRLYDIQHA VVPTRLPLAE FYAELVRTQQ VLNRKHLGWS
ALRGAAGQAA HLLVRGQTNF VRMLWKFNSV FDPRLQLADH EREVKYAMSP PPAMHGAPAI
DMKTVYIHGP IGRKGRKIDD ATERFVDETR MGTG
//
