ID A0A1A5YCC0_9BACL Unreviewed; 730 AA.
AC A0A1A5YCC0;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=A7K91_25235 {ECO:0000313|EMBL:OBR63256.1};
OS Paenibacillus oryzae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1844972 {ECO:0000313|EMBL:OBR63256.1, ECO:0000313|Proteomes:UP000092024};
RN [1] {ECO:0000313|EMBL:OBR63256.1, ECO:0000313|Proteomes:UP000092024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1DrF-4 {ECO:0000313|EMBL:OBR63256.1,
RC ECO:0000313|Proteomes:UP000092024};
RA Zhang J., Zhang X.;
RT "Paenibacillus oryzae. sp. nov., isolated from the rice root.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBR63256.1}.
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DR EMBL; LYPA01000074; OBR63256.1; -; Genomic_DNA.
DR RefSeq; WP_068686522.1; NZ_LYPA01000074.1.
DR AlphaFoldDB; A0A1A5YCC0; -.
DR STRING; 1844972.A7K91_25235; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000092024; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000092024}.
FT DOMAIN 29..286
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 650..726
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 479
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 549
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 367..368
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 444
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 477..481
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 527
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 549
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 730 AA; 82311 MW; 89A877550D091246 CRC64;
MTITYLKDKN VFHLQSKNVS YVLGVTKQGH LAHYYWGKRI RGGHLSQLLE LQERASFSPS
TDMEQLAYSL DTLPQEYPSF GSSDFRQPAF QAQLQNGTTV MELLYESHTI RQGKPGLEGL
PAVYVESQEE AQTLEITVKD ELSGLKAILS YTAFADHPAI ARSVRFVNTS AVPLNLLRAY
SVSLDMPHDS FEMLQLSGAW VRERHVHKRK LVPGLQAVES RRGSSSHMQN PFIALLAEGT
TETFGEAYGL SFVYSGSFKA GVEVDQFHTA RLFAGINDFD FNWLLKGGES FQTPEAILVY
SDEGIGGMSR GYHDLYRTRL CRGEFRDKAR PILINNWEAT YFNFNADKIE AIATAGQQLG
IELFVLDDGW FGKRDSDNSS LGDWFVDRNK LPNGLPDLVQ RVKNLNMEFG LWFEPEMISP
ESELYRNHPD WCLHVEGRRR TQARRQLVLD LSRADVQDYI VTVVSDILSS APITYVKWDM
NRNMTEIGSA LLPAERQRET AHRYMLGLYS VLERITTAFP HILFESCSGG GGRFDPGMLY
YMPQTWTSDN TDAVSRLKIQ YGTSIVYPVS AMGSHVSAVP NHQVNRITSL STRGHVALSG
NFGYELDLTG FSEEEQVEVK AQVALYKEVR HLVQFGDFYR LLSPFEGNDT AWMFVAKDKS
EAFAVFVTVL QEPNPVLGRF RLQGLDAGKN YILKGSETVY GGDELMYAGL PKPQFHGDFS
STVFHFKAVD
//
