ID A0A1A5YPZ6_9BACL Unreviewed; 934 AA.
AC A0A1A5YPZ6;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=A7K91_22655 {ECO:0000313|EMBL:OBR67677.1};
OS Paenibacillus oryzae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1844972 {ECO:0000313|EMBL:OBR67677.1, ECO:0000313|Proteomes:UP000092024};
RN [1] {ECO:0000313|EMBL:OBR67677.1, ECO:0000313|Proteomes:UP000092024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1DrF-4 {ECO:0000313|EMBL:OBR67677.1,
RC ECO:0000313|Proteomes:UP000092024};
RA Zhang J., Zhang X.;
RT "Paenibacillus oryzae. sp. nov., isolated from the rice root.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBR67677.1}.
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DR EMBL; LYPA01000032; OBR67677.1; -; Genomic_DNA.
DR RefSeq; WP_068680579.1; NZ_LYPA01000032.1.
DR AlphaFoldDB; A0A1A5YPZ6; -.
DR STRING; 1844972.A7K91_22655; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000092024; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Pyruvate {ECO:0000313|EMBL:OBR67677.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000092024}.
FT ACT_SITE 156
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 590
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 934 AA; 107183 MW; 774E6581FEFA8FE1 CRC64;
MSEQPIATTT ASRTSANNLL RRDVRFLGNI LGDVLVHQGG RELLDIVERI REQSKALRAG
FLPELFEQFK TTVNGLSPEI RHQVIRAFAI YFQLVNIAEQ NHRIRRKRDY EVSAGETVQR
GSIESAIKEL SELGLDVAAV SEIMKVISLE LVITAHPTEA TRRVVLDVHK RIADDMMQLD
NPTLTHRERG KLKEKLLNEV LILWQTDELR DRKPTVIDEV RNGLYFFDET LFEAIPSVYE
ELERCLTKYY PGEHWHVPDY LRFGSWIGGD RDGNPSVTSS VTWETLQLHR QLALRKYEEK
LKNLVDLLSF STNLVTVSEE LVESIRNDRS HVELRCVDPW RNAKEPYRIK LAFMLEKLAN
TRDESLKGTP TRYNHPSELR EDLLIIDRSL RHHFADYVAD TDLAKLVRQV ELFGFHLMAL
DVRQHSQEHQ NAMTEILQKM HVTENYGALS EAEKTELLHR LLNDPRPLTS SHLDYSESTR
ECLDVYHTIY RAQQEFGEGC ITSYLISMTE AASDMLEVMV FAKEVGLFRQ ESDGTVRCTL
QSVPLFETID DLHAAPAIMK ELFELPVYRK AVEARGNMHE IMLGYSDSNK DGGVVTANWE
LRVALKEITA AGKEYDVKLK FFHGRGGALG RGGMPLNRSI LAQPPHTVGA GIKITEQGEV
LSSRYSLEGI AYRSLEQATW ALITSARLNK YPQQEPEAER EWDEIARSIS ETALTKYQDL
IFRDPDFMTF FKESTPLPEV GELNIGSRPS KRKNSDRFED LRAIPWVFAW TQSRYLLPAW
YAAGTALKQY VGDSEERLET LRTMYEQFPF FSSLIDNLQM ALAKADLLIA KEYANMISDD
TIRQRIFSQI DEEYRVTSEL ILKITKQDEI LDNVPVIQES IRLRNPYVDP LSYMQVQLLS
ELRALRDKDE DDPELLREVL LTINGIAAGL RNTG
//
