ID A0A1A6ABC7_9TREE Unreviewed; 794 AA.
AC A0A1A6ABC7;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
GN ORFNames=I303_01564 {ECO:0000313|EMBL:OBR87362.1};
OS Kwoniella dejecticola CBS 10117.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Kwoniella.
OX NCBI_TaxID=1296121 {ECO:0000313|EMBL:OBR87362.1, ECO:0000313|Proteomes:UP000078595};
RN [1] {ECO:0000313|EMBL:OBR87362.1, ECO:0000313|Proteomes:UP000078595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 10117 {ECO:0000313|EMBL:OBR87362.1,
RC ECO:0000313|Proteomes:UP000078595};
RG The Broad Institute Genome Sequencing Platform;
RA Cuomo C., Litvintseva A., Chen Y., Heitman J., Sun S., Springer D.,
RA Dromer F., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A.,
RA Alvarado L., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Pearson M., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Cryptococcus dejecticola CBS10117.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC ChEBI:CHEBI:456215; EC=6.3.1.14;
CC Evidence={ECO:0000256|ARBA:ARBA00001559};
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DR EMBL; KI894028; OBR87362.1; -; Genomic_DNA.
DR RefSeq; XP_018265204.1; XM_018404923.1.
DR AlphaFoldDB; A0A1A6ABC7; -.
DR STRING; 1296121.A0A1A6ABC7; -.
DR GeneID; 28965263; -.
DR VEuPathDB; FungiDB:I303_01564; -.
DR OrthoDB; 103959at2759; -.
DR Proteomes; UP000078595; Unassembled WGS sequence.
DR GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR CDD; cd01994; Alpha_ANH_like_IV; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR Gene3D; 3.30.1330.40; RutC-like; 2.
DR InterPro; IPR002761; Diphthami_syn_dom.
DR InterPro; IPR030662; DPH6/MJ0570.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR035959; RutC-like_sf.
DR InterPro; IPR006175; YjgF/YER057c/UK114.
DR NCBIfam; TIGR00290; MJ0570_dom; 1.
DR PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR Pfam; PF01902; Diphthami_syn_2; 1.
DR Pfam; PF01042; Ribonuc_L-PSP; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF55298; YjgF-like; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000078595}.
FT DOMAIN 15..246
FT /note="Diphthamide synthase"
FT /evidence="ECO:0000259|Pfam:PF01902"
FT REGION 649..681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 794 AA; 87094 MW; EDD81F3E2BACFA91 CRC64;
MSDPCATSLP GTKHKVIGLV SGGKDSCFNL MHCVANGHKI VALATLTPEP GVDELDSHLY
QSVGTNLIHL IAKSMDLPLY TRVIKGKAVS KGPEYGSRLK GGEGSGEGGD ETEDLTKLLV
DVMAKHPEAT ALSSGAILST YQRLRIEHVC SRLNLVSLAY LWQSKQMPLL ERMLSCGMEV
VLMKVAGVGL GTEVVGKQLA DIMPLLRKLE ARYGSHPAGE GGEYETFTLS TPLFSHRIKL
KTVRTIVTDP EPYPVAYLKI EDAELEEKEG WVKPSVAELR DMLGLDNHEQ AEEGLDEDSI
ELLEELKRDV GLETLKIDNV NNLNTLRDKI DRRNDSSSDL HSANDEGQKV RFARQGRWFS
ASISGITQEG EDVGQELRKC FDDISETLKS NNLSLPLHTT HITLLLSSMS LFVPANEMYK
TYFGTSPPSR ATVAVPLASG RVRIEIVGFD DTLPTSPSSS STVSTVSAEQ SQQQLSSVGG
RNALHVQGLS YWAPANIGPY SQAVMVNQRL HLAGQIPLLP PSLTLPIPPS PSPSPYPHQA
ILALQHVSKI IDVLRSKNYT GGGWEGWVES CTAWWASSGC VSGTGSRAEA KSVLQRDAEG
LKVFRKAWEL WIERNGDSRT PVLFVQAEEL PKSALVEYQV NVHTGRRGVD PISSMAGMSG
TSGTIERGRD DDAEEDDEDH ELEPIYEQAE ELERRIRWEA YSTTSKLNQG SRGVVFVRAI
DELSRIWDIT RIARTVEKAI TVRIYHLPGT ENHLSSLLSM SMSQLKCNYT LIPVLSIHDK
AGEEAVVALE IFGI
//
