GenomeNet

Database: UniProt
Entry: A0A1A7BK59_9SPHN
LinkDB: A0A1A7BK59_9SPHN
Original site: A0A1A7BK59_9SPHN 
ID   A0A1A7BK59_9SPHN        Unreviewed;       623 AA.
AC   A0A1A7BK59;
DT   05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2016, sequence version 1.
DT   31-JUL-2019, entry version 19.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN   ORFNames=I603_1305 {ECO:0000313|EMBL:OBV11862.1};
OS   Porphyrobacter dokdonensis DSW-74.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Erythrobacteraceae; Porphyrobacter.
OX   NCBI_TaxID=1300349 {ECO:0000313|EMBL:OBV11862.1, ECO:0000313|Proteomes:UP000092484};
RN   [1] {ECO:0000313|EMBL:OBV11862.1, ECO:0000313|Proteomes:UP000092484}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSW-74 {ECO:0000313|EMBL:OBV11862.1,
RC   ECO:0000313|Proteomes:UP000092484};
RA   Kim J.F., Song J.Y.;
RT   "Genome sequence of Porphyrobacter dokdonensis DSW-74.";
RL   Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA
CC       replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC       ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00974};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC       Rule:MF_00974};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC       domain that contains the primase activity, and a C-terminal DnaB-
CC       binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:OBV11862.1}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; LZYB01000002; OBV11862.1; -; Genomic_DNA.
DR   RefSeq; WP_068863236.1; NZ_LZYB01000002.1.
DR   EnsemblBacteria; OBV11862; OBV11862; I603_1305.
DR   PATRIC; fig|1300349.4.peg.1300; -.
DR   BioCyc; GCF_001677335:I603_RS06445-MONOMER; -.
DR   Proteomes; UP000092484; Unassembled WGS sequence.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000092484};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709304};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN      255    337       Toprim. {ECO:0000259|PROSITE:PS50880}.
FT   ZN_FING      38     62       CHC2-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00974}.
FT   REGION      418    446       Disordered. {ECO:0000256|SAM:MobiDB-
FT                                lite}.
FT   REGION      604    623       Disordered. {ECO:0000256|SAM:MobiDB-
FT                                lite}.
SQ   SEQUENCE   623 AA;  68321 MW;  9EF6612E392806B7 CRC64;
     MAITPQWKDE LRARITLSSL VQRSVKLTRA GREWKGCCPF HDEKTPSFYV NDQKQFYHCF
     GCGAHGDAIS WMVDQQGLQF MDAIKELAAM AGMEVPAPDP QMAKRAEQRA SLIDVTEAAQ
     RFFVESLAAP GGRVARDYLQ RRGFSPAICR EFGFGWAPDE RQALPKALAQ FSDEMLEGAG
     MRAHTEQGER YDRFRGRVML PIQDARGRVI AFGGRILDKR DGIAKYLNSP DTELFDKGRT
     LYNLHRAAPA ARQSGRVVVV EGYMDVIALA NAGIADAVAP LGTALTEMQL ELLWRMVEVP
     VLCFDGDAAG QRAAMRAIAR ALPLLAPMRS LAIVRLPAGL DPDDLIKTRG KAAMERILEK
     PASLIDTLWE FERDAQPLST PEAKAGLKAR LMAHVDTIAD PEIKALYRRE LSDRFSAFAY
     PPRPPRSAPM QRGPRRAAAP APAGLSDDAR ARLSAVMSGG QRQELFQAVI VGLARFPREI
     RRHAEALSRL ASYDPKSAPA IESLFELAER LDSRAPTAIC ESLGHPAPPA DIRYAFLDEG
     TSTGDACEEL AEAVSLLVER PALEAALAAT IARFDSDPEG SFAEQTRLRT QLMTVDERLK
     AFGRRKAAPV ATGDDSAPAE DMG
//
DBGET integrated database retrieval system