ID A0A1A7QFU9_9SPHN Unreviewed; 756 AA.
AC A0A1A7QFU9;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN ORFNames=A9995_10070 {ECO:0000313|EMBL:OBX18890.1};
OS Erythrobacter sp. QSSC1-22B.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter.
OX NCBI_TaxID=1860125 {ECO:0000313|EMBL:OBX18890.1, ECO:0000313|Proteomes:UP000092728};
RN [1] {ECO:0000313|EMBL:OBX18890.1, ECO:0000313|Proteomes:UP000092728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=QSSC1-22B {ECO:0000313|EMBL:OBX18890.1,
RC ECO:0000313|Proteomes:UP000092728};
RA Bowman J.P.;
RT "Draft genome of Erythrobacter sp. QSSC1-22B.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBX18890.1}.
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DR EMBL; LZRP01000005; OBX18890.1; -; Genomic_DNA.
DR RefSeq; WP_067612356.1; NZ_LZRP01000005.1.
DR AlphaFoldDB; A0A1A7QFU9; -.
DR STRING; 1860125.A9995_10070; -.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000092728; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:OBX18890.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000092728};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OBX18890.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 23..168
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
SQ SEQUENCE 756 AA; 84420 MW; EDD577A4D1171B50 CRC64;
MKAAASARLI LTRLHEVMAS RLHAQAKLDQ VVEIIGESLT SEVCSIYLLR EGALELFATR
GLNPDAVHVT RLGLGEGLIG TIAKNIETLN LAEAKAHPEF LYHPETGEEK FHSFAGVPIV
YRERAVGALS VQHVEPRRYE DVEIEALQTT AMILSELIAH KELVDLEHPL DLAELPSGPE
MIDGLALVKG LSEGVAVFHQ PRVEIDQVVA EDTEAERQRV YRAFDKMRDQ IDAMGKEPEF
GKGGEHDDVL ETYKMFAYDE GWSRRINEAI DSGLTAEAAI ERVQQRTRMR MREIDDPLLA
DRMHDLEDLS NRLLRIVSGQ LGTAARQGLR RDSILVARNL GPAELLEYDR RRLKGVVLEE
GSLTAHMVIV ARAMDIPVLG RAKGVLRRVN EGDEVLLDAD KGCITLRPSP PMREAFDARF
AKKRERQAAY AELRDVEPFT RCGTRINVMM NAGLRDDMSS LAMTGADGVG LFRTEFQFLV
SSTLPQRDRQ TRLYRDVLDA AGDKPVIFRT IDIGGDKAVP YIDASMGERE ENPAMGWRAL
RLSLERGGLL KAQARSLLEA ASGRTLNVMF PMVSEPWEFD AAKEVFDEQL AWLKDRRKRL
PEEIRYGAML EVPALAEVLD LMLPKLSFLS VGTNDLTQFL FAADRANPKL AERYDWMSPS
ILRFLQRIAK GVVGHPVDLS VCGEMGGRRL EALALLGIGY RRLSITPASV GPIKELVRKI
DLDELSGVMQ GWLASPPPDM RVALQDWAAE REIETD
//
