ID A0A1A7R2A5_9FLAO Unreviewed; 532 AA.
AC A0A1A7R2A5;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN ORFNames=LX77_00107 {ECO:0000313|EMBL:RAJ27535.1};
OS Gelidibacter algens.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Gelidibacter.
OX NCBI_TaxID=49280 {ECO:0000313|EMBL:RAJ27535.1, ECO:0000313|Proteomes:UP000248987};
RN [1] {ECO:0000313|EMBL:RAJ27535.1, ECO:0000313|Proteomes:UP000248987}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12408 {ECO:0000313|EMBL:RAJ27535.1,
RC ECO:0000313|Proteomes:UP000248987};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699,
CC ECO:0000256|RuleBase:RU000555};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAJ27535.1}.
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DR EMBL; QLLQ01000001; RAJ27535.1; -; Genomic_DNA.
DR RefSeq; WP_066435735.1; NZ_QLLQ01000001.1.
DR AlphaFoldDB; A0A1A7R2A5; -.
DR STRING; 49280.A9996_12825; -.
DR OrthoDB; 9768429at2; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000248987; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW ECO:0000256|RuleBase:RU000555};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU000555}.
FT DOMAIN 19..71
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 163..407
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 413..532
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 167
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 447
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 532 AA; 61323 MW; 3962B9C78C52C91C CRC64;
MENNILSLPK IQYSKDVKNY YPEILTDEAL EFLTTLHEKF NQGRLELLKS REEQQKQFDQ
GAFPDFPAET ERIREGDWTA GAIPQDLLDR RVEITGPVDR KMVINALNSG AKTFMADLED
SNAPTWQNAI EGQQNLIDAN NRTISLHDVK RNKSYTLNAD PAVLMVRPRG LHLNERHMLI
NKEEMSGSLV DFGLYVFHNT KTLLDNNSAP YFYLPKLEHY TEARWWNQVF DFAENYMKVP
KGTFKATVLV ETITASFQLD EIIYELKDHI VGLNCGRWDY IFSYIKKFRN HDNFVVPNRD
QVTMTTPFMA AYSKLVIRRC HKRGIHAIGG MAAQIPIKNN PEANKAALEK VRVDKEREVK
NGHDGTWVAH PALVEVAMKE FDKHMPTANQ FHVTRDDVSI TKEDLVELPI GTVTEEGVRK
NINVGLLYTE AWLRGHGAVA LYNLMEDAAT AEISRTQIWQ WLKNEVVLED GRKFSRTLYD
TIFDDEVEKL ITEFGEENIK NTKFSLAIEL FNNLVTNDDF EEFLTLPAYQ HI
//
