ID A0A1A9HSB1_9BURK Unreviewed; 387 AA.
AC A0A1A9HSB1;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Mandelate racemase {ECO:0000313|EMBL:ANH70854.1};
GN ORFNames=ABE85_23180 {ECO:0000313|EMBL:ANH70854.1};
OS Mitsuaria sp. 7.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Roseateles.
OX NCBI_TaxID=1658665 {ECO:0000313|EMBL:ANH70854.1, ECO:0000313|Proteomes:UP000078154};
RN [1] {ECO:0000313|EMBL:ANH70854.1, ECO:0000313|Proteomes:UP000078154}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=7 {ECO:0000313|EMBL:ANH70854.1,
RC ECO:0000313|Proteomes:UP000078154};
RA Bao W., Leung F.C.C., Jiang J.;
RT "Complete Genome Sequence of Mitsuaria sp. Strain 7, a Lignin-degrading
RT Bacterium Isolated from Forest Soil.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR634611-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR634611-3};
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DR EMBL; CP011514; ANH70854.1; -; Genomic_DNA.
DR RefSeq; WP_067283399.1; NZ_CP011514.1.
DR AlphaFoldDB; A0A1A9HSB1; -.
DR STRING; 1658665.ABE85_23180; -.
DR KEGG; miu:ABE85_23180; -.
DR PATRIC; fig|1658665.3.peg.4745; -.
DR OrthoDB; 9802699at2; -.
DR Proteomes; UP000078154; Chromosome.
DR GO; GO:0047808; F:D(-)-tartrate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03326; MR_like_1; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034611; D-tartrate_dehydratase.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080:SF5; D(-)-TARTRATE DEHYDRATASE; 1.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDF00118; D-tartrate_dehydratase; 1.
DR SFLD; SFLDG00179; mandelate_racemase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR634611-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR634611-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000078154}.
FT DOMAIN 161..258
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 182
FT /note="acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT ACT_SITE 320
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT BINDING 19
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 53
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 99
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 154
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 180
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 211
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 237
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 237
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 263
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 263
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 320
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 180
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 290
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 339
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
SQ SEQUENCE 387 AA; 43044 MW; F18227218C1CE19B CRC64;
MKIVEIREKT LPISSPIRNA YIDFSKMTLS LVAVITDVIR DGKPVVGYGF NSNGRYGQGT
LMRERFIPRI LEADPDSLLD SRGILDAHKV WDAMFTNEKP GGHGERSVAI GTIDMAVWDA
VAKIEGKPLF QLLAERYGTG TPDRKVFVYA AGGYYYPGQD HGKLKDEMRS YIDRGYTVVK
KKIGGASMDE DLRRIDSILS VLGDGQRLCV DANGRFDLDT AIAYAKALSK YDLFWYEEAG
DPLDFELQAT LRNYYANPMA TGENLFSMQD ARNLIRYGGM RPDRDWLQFD CALSYGLVEY
LRTLDMLKEH GWSASRCVPH GGHQMSLNIA AGLGLGGNES YPDLFQPFGG FPDGVKVENS
YVTLPDLPGI GFEGKSDLYA VMRALSD
//
