ID   A0A1A9ZLS4_GLOPL        Unreviewed;       808 AA.
AC   A0A1A9ZLS4;
DT   05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2016, sequence version 1.
DT   24-JAN-2024, entry version 27.
DE   RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE            EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE   AltName: Full=ATP-binding domain-containing protein 4 {ECO:0000256|ARBA:ARBA00031202};
DE   AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE   AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
DE   AltName: Full=Protein DPH6 homolog {ECO:0000256|ARBA:ARBA00032849};
OS   Glossina pallidipes (Tsetse fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Hippoboscoidea;
OC   Glossinidae; Glossina.
OX   NCBI_TaxID=7398 {ECO:0000313|EnsemblMetazoa:GPAI018618-PA, ECO:0000313|Proteomes:UP000092445};
RN   [1] {ECO:0000313|Proteomes:UP000092445}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAEA {ECO:0000313|Proteomes:UP000092445};
RA   Aksoy S., Warren W., Wilson R.K.;
RL   Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblMetazoa:GPAI018618-PA}
RP   IDENTIFICATION.
RC   STRAIN=IAEA {ECO:0000313|EnsemblMetazoa:GPAI018618-PA};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC         AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC         H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC         COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC         ChEBI:CHEBI:456215; EC=6.3.1.14;
CC         Evidence={ECO:0000256|ARBA:ARBA00001559};
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00005156}.
CC   -!- SIMILARITY: Belongs to the Diphthine--ammonia ligase family.
CC       {ECO:0000256|ARBA:ARBA00008496}.
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DR   AlphaFoldDB; A0A1A9ZLS4; -.
DR   STRING; 7398.A0A1A9ZLS4; -.
DR   EnsemblMetazoa; GPAI018618-RA; GPAI018618-PA; GPAI018618.
DR   VEuPathDB; VectorBase:GPAI018618; -.
DR   UniPathway; UPA00559; -.
DR   Proteomes; UP000092445; Unassembled WGS sequence.
DR   GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017183; P:protein histidyl modification to diphthamide; IEA:UniProtKB-UniPathway.
DR   CDD; cd01994; Alpha_ANH_like_IV; 1.
DR   CDD; cd06155; eu_AANH_C_1; 1.
DR   CDD; cd06156; eu_AANH_C_2; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR   Gene3D; 3.30.1330.40; RutC-like; 2.
DR   InterPro; IPR002761; Diphthami_syn_dom.
DR   InterPro; IPR030662; DPH6/MJ0570.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR035959; RutC-like_sf.
DR   InterPro; IPR006175; YjgF/YER057c/UK114.
DR   NCBIfam; TIGR00290; MJ0570_dom; 1.
DR   PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR   PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR   Pfam; PF01902; Diphthami_syn_2; 1.
DR   Pfam; PF01042; Ribonuc_L-PSP; 2.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR   SUPFAM; SSF55298; YjgF-like; 2.
PE   3: Inferred from homology;
FT   DOMAIN          19..147
FT                   /note="Diphthamide synthase"
FT                   /evidence="ECO:0000259|Pfam:PF01902"
FT   REGION          463..489
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   808 AA;  89581 MW;  FF1F537C0D27B614 CRC64;
     MKTFLRSIGL FTFHSLSTKE LHIEAVAVGA ILSDYQRVRV ENVCNRLSLM SLAYLWRRDQ
     TELLQEMIDC QVHAVLIKNS FDKVASLGLV PDRHLGKSLH EIQPHLLKMR DKYGLNVCGE
     GGEYETFTLD CPLFKQRIVI DDVQTVISSA DPICPVGYIN FTKLSLQPKD MQQSCDVIVK
     KSLDYISDLN ESTYSDLSDP DLSETELELI EKETRLRESL SQNELISRSN SFGRHLATST
     SPIPIVTKSA SVDEHTPLNL LSSSAALCAA SLHAGTFGSP RLLGSSTAAV CGSLSLAVSS
     IGLSAIGTTL GKDGGMSSTT VGGGNGGGTL IFTQPPSPLK YEREFHPLNN RAMVAINCKG
     WIWVAGIQGC ASTLEEGFTK ALDSLRSLAV EHNYEMTDYC YVTLYVRSIS EYPALNRIYG
     QALNFQNPPT RVCVECPLPD ECHVIIEAIA YKKPQRGRRS TISVNDTDVS SESQQAQNTS
     INNGELPNKR NTMHVQSISH WAPANIGPYS QSTKINEITY ISGQIALVPG SMTIIDGGIR
     PQCKLTLRHI SRIAKAMNAQ GQLRDVVHGI CFITHPAFIG EARRQWERRT TNAIMDYIVV
     PALPREALVE WQVWAHTHND RFEYEETGCS ISDYTISIRR RWNYENNCAA IICYVSTGLA
     SSTTQLTQLS EDILNNHRHL AQNLKADHLE EILTYVVNRL LKDYPSLKRS NENLCTSHDG
     ISTTENNVNA IHNCTSNSAN VDIILPPAIH LKVFYQVTAA PPVDLLLMAL NEYREKCEQT
     ANIVFTVLPA CSLHNFSTFL SICGVRHE
//