ID A0A1B0FKG2_GLOMM Unreviewed; 1235 AA.
AC A0A1B0FKG2;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
OS Glossina morsitans morsitans (Savannah tsetse fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Hippoboscoidea;
OC Glossinidae; Glossina.
OX NCBI_TaxID=37546 {ECO:0000313|EnsemblMetazoa:GMOY004289-PA, ECO:0000313|Proteomes:UP000092444};
RN [1] {ECO:0000313|Proteomes:UP000092444}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Yale {ECO:0000313|Proteomes:UP000092444};
RA Lawson D.;
RT "Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African
RT Trypanosomiasis.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000092444}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Yale {ECO:0000313|Proteomes:UP000092444};
RG International Glossina Genome Initiative W.H.O.;
RA Lawson D.;
RT "Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African
RT Trypanosomiasis.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblMetazoa:GMOY004289-PA}
RP IDENTIFICATION.
RC STRAIN=Yale {ECO:0000313|EnsemblMetazoa:GMOY004289-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; CCAG010018197; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A1B0FKG2; -.
DR STRING; 37546.A0A1B0FKG2; -.
DR EnsemblMetazoa; GMOY004289-RA; GMOY004289-PA; GMOY004289.
DR VEuPathDB; VectorBase:GMOY004289; -.
DR PhylomeDB; A0A1B0FKG2; -.
DR Proteomes; UP000092444; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035}.
FT DOMAIN 32..655
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 716..870
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1235 AA; 142899 MW; 14186E0E59AA6C1E CRC64;
MVSSKRESPD KLTREDVCRV PETICFPEEE DQILNDWREK KIFETCMKLS KGKPKYTFYD
GPPFATGLPH YGHILAGTIK DIVTRYAYQQ GYHVDRRFGW DCHGLPVEFE IDKLLGVRGP
EDVEKMGIAT YNAECRKIVT RYAKEWESIV TRMGRWIDFK NDYKTLYPWY MESIWWVFKQ
LYDKGLVYEG VKVMPYSTAC TTPLSNFESG QNYKEVVDPC VVIALEAVGY ENTYFLIWTT
TPWTLPSNFA CCVNPKMEYV KVKDVKTLRF YILAKCRLSY VYKTEEDYEV TEEFLGETLA
GIHYKPLFPY FANRGELVKA FKVLVDNYVT DDSGTGIVHN APYFGEDDHR VCLVADLITK
SSPVICPVDD AGRFTEEVTH FKGLYVKDAD KHIIAFLKAS GNLVSAGQVK HSYPFCWRSD
TPLIYKAVPS WFIRVEHMSK NLLACSSLTY WVPDFVKEKR FGNWLKEARD WAISRNRYWG
TPIPIWRSLN GEETVCVGSI KEVEQLSGKL ITDLHRETVD EIEIPSAVLG NPPLRRITPV
FDCWFESGSM PFAQQHFPFE NEKDFMSNFP ADFIAEGIDQ TRGWFYTLLV ISTALFNKAP
FKNLIANGLV LAADGQKMSK RKKNYPDPLE VVQKYGADAL RLYLINSPVV RAENLRFKEE
GVRDIVKDVF LPWYNAYRFL LQNIARYEKE ELQSASLYMY DKERHLKNYE KSSIIDVWIL
SFKESLLDFF ATEMKMYRLY TVVPRLTKFI DQLTNWYVRL NRRRIKGEMG KEECIQSLDT
LYDVLFTMVK MMAPFTPFLT EYIFKRLVLF QPKNAVDNIE SIHYQMMPIS NRKFIRAEVE
KSVAIMQAII ELGRVMRDRR TLPVKYPVSE IIVIHKDPKC LEAIKSLEDL ILGELNVRKL
TLTSDKEKYG VTMRAEPDHK ILGQRLKSNF KSVLSAIKAL TDDDIQKHLS RGYFEIDQQR
IELEEVRIIY CTSPQMGGGN FEAHSDNEVL VLMDMTPNEE LLAEGLAREI INRVQKLKKK
GHLIPTDPVV IYYELTAPPQ KQADIEHITK VMETYYSMIT ATIKSNFAKY SAEEAKNKRI
IISESVDLKG IDLKLTICSM EDVQLPLVHW VNVALAHDLQ PRYGRSNKAS LLLRDVATNN
ILTIEKLRHE IDVLFGLYGI NYSIYIMQTE EDAVSKLTAL DLSANGKLLV ISYRIEEAER
FLKETSASYM PYCRFINREG STVFTENPLG FPLDA
//
