ID A0A1B0GHM6_LUTLO Unreviewed; 1418 AA.
AC A0A1B0GHM6;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Alkaline phosphatase {ECO:0000256|ARBA:ARBA00012647, ECO:0000256|RuleBase:RU003947};
DE EC=3.1.3.1 {ECO:0000256|ARBA:ARBA00012647, ECO:0000256|RuleBase:RU003947};
OS Lutzomyia longipalpis (Sand fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Psychodoidea; Psychodidae;
OC Lutzomyia; Lutzomyia.
OX NCBI_TaxID=7200 {ECO:0000313|EnsemblMetazoa:LLOJ001549-PA, ECO:0000313|Proteomes:UP000092461};
RN [1] {ECO:0000313|Proteomes:UP000092461}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Jacobina {ECO:0000313|Proteomes:UP000092461};
RA Richards S., Qu C., Dillon R., Worley K., Scherer S., Batterton M.,
RA Taylor A., Hawes A., Hernandez B., Kovar C., Mandapat C., Pham C., Qu C.,
RA Jing C., Bess C., Bandaranaike D., Ngo D., Ongeri F., Arias F., Lara F.,
RA Weissenberger G., Kamau G., Han H., Shen H., Dinh H., Khalil I., Jones J.,
RA Shafer J., Jayaseelan J., Quiroz J., Blankenburg K., Nguyen L., Jackson L.,
RA Francisco L., Tang L.-Y., Pu L.-L., Perales L., Lorensuhewa L.,
RA Munidasa M., Coyle M., Taylor M., Puazo M., Firestine M., Scheel M.,
RA Javaid M., Wang M., Li M., Tabassum N., Saada N., Osuji N., Aqrawi P.,
RA Fu Q., Thornton R., Raj R., Goodspeed R., Mata R., Najjar R., Gubbala S.,
RA Lee S., Denson S., Patil S., Macmil S., Qi S., Matskevitch T.,
RA Palculict T., Mathew T., Vee V., Velamala V., Korchina V., Cai W., Liu W.,
RA Dai W., Zou X., Zhu Y., Zhang Y., Wu Y.-Q., Xin Y., Nazarath L., Kovar C.,
RA Han Y., Muzny D., Gibbs R.;
RT "Whole Genome Assembly of Lutzomyia longipalpis.";
RL Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MBC1169273.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Jacobina {ECO:0000313|EMBL:MBC1169273.1};
RC TISSUE=Midgut {ECO:0000313|EMBL:MBC1169273.1};
RA Coutinho-Abreu I.V., Serafim T.D., Meneses C., Kamhawi S., Oliveira F.,
RA Valenzuela J.G.;
RT "Leishmania infection induces a limited differential gene expression in the
RT sand fly midgut.";
RL BMC 21:608-608(2020).
RN [3] {ECO:0000313|EnsemblMetazoa:LLOJ001549-PA}
RP IDENTIFICATION.
RC STRAIN=Jacobina {ECO:0000313|EnsemblMetazoa:LLOJ001549-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.1;
CC Evidence={ECO:0000256|RuleBase:RU003947};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 1 Mg(2+) ion. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- SIMILARITY: Belongs to the alkaline phosphatase family.
CC {ECO:0000256|ARBA:ARBA00005984, ECO:0000256|RuleBase:RU003946}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJWK01005343; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJWK01005344; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJWK01005345; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJWK01005346; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; GITU01000570; MBC1169273.1; -; Transcribed_RNA.
DR EnsemblMetazoa; LLOJ001549-RA; LLOJ001549-PA; LLOJ001549.
DR VEuPathDB; VectorBase:LLOJ001549; -.
DR OrthoDB; 35876at2759; -.
DR Proteomes; UP000092461; Unassembled WGS sequence.
DR GO; GO:0004035; F:alkaline phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd16012; ALP; 3.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 3.
DR InterPro; IPR001952; Alkaline_phosphatase.
DR InterPro; IPR018299; Alkaline_phosphatase_AS.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR PANTHER; PTHR11596; ALKALINE PHOSPHATASE; 1.
DR PANTHER; PTHR11596:SF94; ALKALINE PHOSPHATASE; 1.
DR Pfam; PF00245; Alk_phosphatase; 3.
DR PRINTS; PR00113; ALKPHPHTASE.
DR SMART; SM00098; alkPPc; 3.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 3.
DR PROSITE; PS00123; ALKALINE_PHOSPHATASE; 3.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|RuleBase:RU003947};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00022622};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR601952-2};
KW Membrane {ECO:0000256|ARBA:ARBA00022622};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601952-2};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR601952-2}.
FT ACT_SITE 1031
FT /note="Phosphoserine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-1"
FT BINDING 987
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 987
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1096
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1252
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1257
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1261
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1299
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 1376
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
SQ SEQUENCE 1418 AA; 158829 MW; C8C37C9D95C5C991 CRC64;
MEEPRGVLNS VDRLNSAEYV GRIYRRNVQF DEKIPDSWFN VDEELDDGSH ADVDTPNHFT
LNLRMEFPKE FTKKQPEEKI APIVKNPMWI DKLAIIKPPP MCPTFDQKDD LGHKYLENDG
HLRILPPVGL YEGPMVGVPY RAYSPDYAQM LFETLQRHPH FQTSHRLEDG YLTDSPIKQT
QDVIEFIKAL TESKNKEKNF EKRHIKEYNS NKDIIDEAIV DEDALSNIFL EQDREFEKYL
EQIGYSGQVE MFDLQMDKSP ASQSIFYSNP KKSNNFQGQS KDSLEQIKLQ GLHIGEGAGE
EISKVLMHIS PKNLLQQDVS AGRSIGYSWE WNSEYWLNLG RQYVLDSINR QLNTNRAKNV
VFFLGDGMSI PTQFVARMAM GGEEQQLSWE KFPFAGLSKT YCVDSQVADS ACSGTAYWSG
IKANLGTLGV NARVPFGDCY AALDAATHTE SIAKWAMDAG KSVGIVTTAK VTSASPAAAY
AHENDSNIRA TGCDPNMIDD IAKQLILGPI GCRLNVILGG GRGNMRDSKI PDEEGGFGER
NDSRDLISEW LNDGRPNKRY VWNRHDLLNL PYDTESLLGL FESGDMQFYL ESLDRRSLMT
EPTLEEMTRN LLQQDVSAGR SIGYSWEWNS EYWLNLGRQY VLDSINRQLN TNRAKNVVFF
LGDGMSIPTQ FVARMAMGGE EQQLSWEKFP FAGLSKTYCV DSQVADSACS GTAYWSGIKA
NLGTLGVNAR VPFGDCYAAL DAATHTESIA KWAMDAGKSV GIVTTAKVTS ASPAAAYAHV
SNRFWENDSN IRATGCDPNM IDDIAKQLIL GPIGCRLNVI LGGGRGNMRD SKIPDEEGGF
GERNDSRDLI SEWLNDGRPN KRYVWNRHDL LNLPYDTESL LGLFESGDMQ FYLESLDRRS
LMTEPTLEEM TRDYTSEKCR EEISKVLMHI SPKNLLQQDV SAGRSIGYSW EWNSEYWLNL
GRQYVLDSIN RQLNTNRAKN VVFFLGDGMS IPTQFVARMA MGGEEQQLSW EKFPFAGLSK
TYCVDSQVAD SACSGTAYWS GIKANLGTLG VNARVPFGDC YAALDAATHT ESIAKWAMDA
GKSVGIVTTA KVTSASPAAA YAHVSNRFWE NDSNIRATGC DPNMIDDIAK QLILGPIGCR
LNVILGGEEI CETQRFPMRG GFGERNDSRD LISEWLNDGR PNKRYVWNRH DLLNLPYDTE
SLLGLFESGD MQFYLESLDR RSLMTEPTLE EMTRVAINVL KKNQNGFFLF VEGGKIDHGH
HYTLARTASA ETVEMHKAVQ AAREVLSEDD TLIVVTADHS HALSISGYPT RGHDIFGKDD
RFEGKDDLPY MTLNYANGPI LSPQYKEVGR INPDISNYKK WTSPFPFYVP LDIATHAGDD
VGIFASGPFA HLFSGTMEQS HLPHLMAYVM SIGNGPRC
//
