ID A0A1B1MJ47_STRLN Unreviewed; 408 AA.
AC A0A1B1MJ47;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=3-ketoacyl-CoA thiolase/acetyl-CoA acetyltransferase {ECO:0000313|EMBL:ANS68631.1};
DE SubName: Full=Acetyl-CoA C-acyltransferase {ECO:0000313|EMBL:QMV10248.1};
DE EC=2.3.1.16 {ECO:0000313|EMBL:QMV10248.1};
GN ORFNames=GJU35_34385 {ECO:0000313|EMBL:QMV10248.1}, SLCG_2008
GN {ECO:0000313|EMBL:AXG53163.1}, SLINC_6407
GN {ECO:0000313|EMBL:ANS68631.1};
OS Streptomyces lincolnensis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1915 {ECO:0000313|EMBL:ANS68631.1, ECO:0000313|Proteomes:UP000092598};
RN [1] {ECO:0000313|EMBL:ANS68631.1, ECO:0000313|Proteomes:UP000092598}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 2936 {ECO:0000313|EMBL:ANS68631.1,
RC ECO:0000313|Proteomes:UP000092598};
RA Meng S.C.;
RT "Enhancement of antibiotic productionsby engineered nitrateutilization in
RT actinobacteria.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AXG53163.1, ECO:0000313|Proteomes:UP000253884}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LC-G {ECO:0000313|EMBL:AXG53163.1,
RC ECO:0000313|Proteomes:UP000253884};
RX PubMed=29574602; DOI=10.1007/s10295-018-2029-1;
RA Xu Y., Tan G., Ke M., Li J., Tang Y., Meng S., Niu J., Wang Y., Liu R.,
RA Wu H., Bai L., Zhang L., Zhang B.;
RT "Enhanced lincomycin production by co-overexpression of metK1 and metK2 in
RT Streptomyces lincolnensis.";
RL J. Ind. Microbiol. Biotechnol. 45:345-355(2018).
RN [3] {ECO:0000313|EMBL:QMV10248.1, ECO:0000313|Proteomes:UP000515335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B48 {ECO:0000313|EMBL:QMV10248.1,
RC ECO:0000313|Proteomes:UP000515335};
RX PubMed=32322696;
RA Wang R., Kong F., Wu H., Hou B., Kang Y., Cao Y., Duan S., Ye J., Zhang H.;
RT "Complete genome sequence of high-yield strain S. lincolnensis B48 and
RT identification of crucial mutations contributing to lincomycin
RT overproduction.";
RL Synth Syst Biotechnol 5:37-48(2020).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
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DR EMBL; CP016438; ANS68631.1; -; Genomic_DNA.
DR EMBL; CP022744; AXG53163.1; -; Genomic_DNA.
DR EMBL; CP046024; QMV10248.1; -; Genomic_DNA.
DR RefSeq; WP_067441448.1; NZ_CP046024.1.
DR AlphaFoldDB; A0A1B1MJ47; -.
DR STRING; 1915.SLINC_6407; -.
DR KEGG; sls:SLINC_6407; -.
DR PATRIC; fig|1915.4.peg.7079; -.
DR OrthoDB; 3204099at2; -.
DR Proteomes; UP000092598; Chromosome.
DR Proteomes; UP000253884; Chromosome.
DR Proteomes; UP000515335; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0003988; F:acetyl-CoA C-acyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR43853; 3-KETOACYL-COA THIOLASE, PEROXISOMAL; 1.
DR PANTHER; PTHR43853:SF2; 3-OXOADIPYL-COA_3-OXO-5,6-DEHYDROSUBERYL-COA THIOLASE; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00737; THIOLASE_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:QMV10248.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000092598};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:ANS68631.1}.
FT DOMAIN 8..266
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 275..397
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 96
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 354
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 385
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 408 AA; 43544 MW; FA06C6CDB5C6C1CF CRC64;
MPRTVRDVVF VDGVRTPFGK AGPKGIYHET RADDLVVKAI RELLRRNPGL DPKQIDEVAI
AATTQIGDQG LTLGRTAGIL AGLPQSVPGY SIDRMCAGAL TAVTSTAGSI AFGAYDAVIA
GGVEHMGRHP MGEGVDPNPR FVSEKLVDES ALFMGMTAEN LHDRYPTITK QRADEYAVRS
QEKAAKAYAN GKIQADLVPI SVRRTNAEAG ETGWGLVTAD EPMRPGTTLE NLAGLKTPFR
VHGRVTAGNA AGLNDGATAS IIASEDFARE HNLPVKMRLV SYAFAGVEPE VMGYGPIPAT
EKALAKAGLS IEDINLFEIN EAFAVQVLAF LEHYGIADDD ARVNQYGGAI AYGHPLASSG
VRLMTQLARQ FEEQPEVRYG LTTMCVGFGM GATVIWENPH HKDAGGDK
//
