ID A0A1B3B582_WHEAT Unreviewed; 591 AA.
AC A0A1B3B582;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase {ECO:0000256|ARBA:ARBA00039007};
DE EC=1.13.11.51 {ECO:0000256|ARBA:ARBA00039007};
GN Name=NCED2A {ECO:0000313|EMBL:AOE46767.1};
GN ORFNames=CFC21_067516 {ECO:0000313|EMBL:KAF7060751.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EMBL:AOE46767.1};
RN [1] {ECO:0000313|EMBL:AOE46767.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27091738; DOI=10.1007/s00425-016-2518-2;
RA Son S., Chitnis V.R., Liu A., Gao F., Nguyen T.N., Ayele B.T.;
RT "Abscisic acid metabolic genes of wheat (Triticum aestivum L.):
RT identification and insights into their functionality in seed dormancy and
RT dehydration tolerance.";
RL Planta 244:429-447(2016).
RN [2] {ECO:0000313|EMBL:KAF7060751.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7060751.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5A02G374000.1.cds1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5A02G374000.1.cds1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [4] {ECO:0000313|EnsemblPlants:TraesCS5A02G374000.1.cds1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [5] {ECO:0000313|EMBL:KAF7060751.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7060751.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000256|ARBA:ARBA00036784};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000256|ARBA:ARBA00035929};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000256|ARBA:ARBA00036011};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KX711890; AOE46767.1; -; mRNA.
DR EMBL; CM022223; KAF7060751.1; -; Genomic_DNA.
DR SMR; A0A1B3B582; -.
DR STRING; 4565.A0A1B3B582; -.
DR EnsemblPlants; TraesCS5A02G374000.1; TraesCS5A02G374000.1.cds1; TraesCS5A02G374000.
DR Gramene; TraesCAD_scaffold_014363_01G000300.1; TraesCAD_scaffold_014363_01G000300.1; TraesCAD_scaffold_014363_01G000300.
DR Gramene; TraesCLE_scaffold_024131_01G000300.1; TraesCLE_scaffold_024131_01G000300.1; TraesCLE_scaffold_024131_01G000300.
DR Gramene; TraesCS5A02G374000.1; TraesCS5A02G374000.1.cds1; TraesCS5A02G374000.
DR Gramene; TraesCS5A03G0898000.1; TraesCS5A03G0898000.1.CDS1; TraesCS5A03G0898000.
DR Gramene; TraesKAR5A01G0347580.1; cds.TraesKAR5A01G0347580.1; TraesKAR5A01G0347580.
DR Gramene; TraesPAR_scaffold_010820_01G000400.1; TraesPAR_scaffold_010820_01G000400.1; TraesPAR_scaffold_010820_01G000400.
DR Gramene; TraesRN5A0100910700.1; TraesRN5A0100910700.1; TraesRN5A0100910700.
DR Gramene; TraesROB_scaffold_026821_01G000300.1; TraesROB_scaffold_026821_01G000300.1; TraesROB_scaffold_026821_01G000300.
DR Gramene; TraesWEE_scaffold_122158_01G000100.1; TraesWEE_scaffold_122158_01G000100.1; TraesWEE_scaffold_122158_01G000100.
DR OMA; RTHNIRI; -.
DR OrthoDB; 318119at2759; -.
DR BRENDA; 1.13.11.51; 6500.
DR Proteomes; UP000019116; Chromosome 5A.
DR Proteomes; UP000815260; Chromosome 5A.
DR ExpressionAtlas; A0A1B3B582; baseline and differential.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543:SF26; 9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED3, CHLOROPLASTIC; 1.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR Pfam; PF03055; RPE65; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964, ECO:0000313|EMBL:AOE46767.1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 288
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 337
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 402
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 578
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 591 AA; 64488 MW; A3E055A6BD20CB84 CRC64;
MQTLTASTSV SSIQRHTPRR TGRSGSLSFS ARAVSSPPRA PAPSRFVRGA DAAPAKPLIA
VPKAPAVERQ EKKLNFFQRA AATALDAFEE GFVANVLERP HGLSRTVDPA VQIAGNFAPV
GETPPVQALP VTGRIPPFIN GVYARNGANP HFDPVAGHHL FDGDGMVHAL RIRNGVAETY
ASRFTETERL QQERALGRPM FPKAIGELHG HSGIARLALF YARAACGLID PSRGTGVANA
GLVYFNGHLL AMSEDDIPYH VRVTDDGDLQ TVGRYDFDGQ LECPMIAHPK LDPATGELHA
LSYDVIKKPY LKYFYFKADG TKSADVEIPL DQPTMIHDFA ITENYVVVPD HQVVFKLQEM
LRGGSPVVLD KEKTSRFGVL PKCAADASEM VWVDVPDCFC FHLWNAWEEE ESDEVVVIGS
CMTPADSIFN ESDECLESVL TEIRLNTRTG ESTRRPILAL SEQVNLEVGM VNSNLLGRKT
RYAYLAVAEP WPKVSGFAKV DLATGELTKF EYGEGRFGGE PCFVPMDPAA SRGEDDGYIL
TFVHDEAAGT SELLVVNAAD MRLEATIQLP SRVPYGFHGT FVTGKELESQ A
//
