ID   A0A1B3LPQ0_9PROT        Unreviewed;      1264 AA.
AC   A0A1B3LPQ0;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   27-MAR-2024, entry version 36.
DE   SubName: Full=Nitrate reductase, alpha subunit {ECO:0000313|EMBL:AOF82827.1};
DE            EC=1.7.99.4 {ECO:0000313|EMBL:AOF82827.1};
GN   ORFNames=BSY238_1272 {ECO:0000313|EMBL:AOF82827.1};
OS   Methyloversatilis sp. RAC08.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC   Sterolibacteriaceae; Methyloversatilis.
OX   NCBI_TaxID=1842540 {ECO:0000313|EMBL:AOF82827.1, ECO:0000313|Proteomes:UP000094168};
RN   [1] {ECO:0000313|Proteomes:UP000094168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RAC08 {ECO:0000313|Proteomes:UP000094168};
RA   Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL   Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mo-bis(molybdopterin guanine dinucleotide);
CC         Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC       Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004170}.
CC   -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC       oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR   EMBL; CP016448; AOF82827.1; -; Genomic_DNA.
DR   RefSeq; WP_069038382.1; NZ_CP016448.1.
DR   AlphaFoldDB; A0A1B3LPQ0; -.
DR   STRING; 1842540.BSY238_1272; -.
DR   KEGG; metr:BSY238_1272; -.
DR   PATRIC; fig|1842540.3.peg.1285; -.
DR   OrthoDB; 9759518at2; -.
DR   Proteomes; UP000094168; Chromosome.
DR   GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR   GO; GO:0008940; F:nitrate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR   GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR   CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR   CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR   Gene3D; 3.40.50.12440; -; 1.
DR   Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR   InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR   InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR   InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR   InterPro; IPR006656; Mopterin_OxRdtase.
DR   InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR   InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR   InterPro; IPR006468; NarG.
DR   InterPro; IPR028189; Nitr_red_alph_N.
DR   InterPro; IPR044906; Nitr_red_alph_N_sf.
DR   NCBIfam; TIGR01580; narG; 1.
DR   PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR   PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR   Pfam; PF00384; Molybdopterin; 1.
DR   Pfam; PF01568; Molydop_binding; 1.
DR   Pfam; PF14710; Nitr_red_alph_N; 1.
DR   SMART; SM00926; Molybdop_Fe4S4; 1.
DR   SUPFAM; SSF50692; ADC-like; 1.
DR   SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR   PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR   PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR   PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000313|EMBL:AOF82827.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000094168}.
FT   DOMAIN          43..107
FT                   /note="4Fe-4S Mo/W bis-MGD-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51669"
SQ   SEQUENCE   1264 AA;  141828 MW;  CF7EC7ABA3204DE8 CRC64;
     MSHFLDRLSH FSNPKEAFSG DHGITTGEDR SWEDAYRNRW AHDKIVRSTH GVNCTGSCSW
     KIYVKGGIVT WETQQTDYPR TRWDMPNHEP RGCARGASYS WYLYSANRVK YPMVRGRLLK
     SWREARLAHG PVEAWASIVE NDAKRRDYQQ VRGLGGFVRS SWDEVNEIVA AANVYTIKKH
     GPDRVIGFSP IPAMSMISYA AGSRYLSLIG GVCMSFYDWY CDLPPSSPQI WGEQTDVPES
     ADWYNSTYII AWGSNVPQTR TPDAHFFTEV RYKGAKTVAV TPDYSEVAKL SDIWMKPKQG
     TDAAVAMAMG HVILKEFYFP DSGERSAYFD DYVRRYTDMP MLVMLKEHKL PNGDMILVPD
     RYVRASDFNG KLGQANNPEW KTVAFDDSGK VVLPNGAIGF RWGADGRADE GQWNLEAKEA
     RHGANVKLKL SLLEGEAPSS ETARVGFPYF GGIVSEHFPN NAQTDVLVRT VPVQRIALGK
     AGEKRDALVA TVFDLQVANY GIARGLPGEL AAKDFNDDTP YTPAWQECIT GTPREQLITV
     ARQFAENAHK TKGKSMVIIG AAMNHWYHSD MNYRGVINML MMCGCIGQSG GGWAHYVGQE
     KLRPQTGWTA LAFALDWIRP PRQMNSTSFF YAHTDQWRYE KLGMEEVISP LADKKMYGGS
     MIDYNVRAER MGWLPSAPQL QTNPLQVVRD AASAGMDPKD YAVRGLKDGS LKMSCTDPDH
     PDNWPRNMFV WRSNILGSSG KGHEYFLKHL LGTSNGVQGK DLGKEEAKPT EVVWHDTAPE
     GKLDLLVTLD FRMSTTCLYS DIVLPTATWY EKNDLNTSDM HPFIHPLSTA VDPAWQSRSD
     WDIYKGFARK FSEVCVGHLG VERELVLSPL MHDSPSELAQ PFDVKDWKRG ECELIPGKTA
     PNLVVVERDY PNVYKRFTAL GPLMNKVGNG GKGISWNTQT EVKQLGELNG LVTAEGVTCG
     MPKIDTDIDA CEVVLQLAPE TNGHVAVKAW QALGKQTGLK HEHLALYRED EKIRFRDIQA
     QPRKIISSPT WSGIESETVS YNAGYTNVHE LIPWRTLTGR QQFYMDHPWM IAFGEGFTSY
     RPPVDLKTTA GIHNIKPNGN PEIALNFITP HQKWGIHSTY TDNLLMLTLS RGGPCVWMSE
     DDAKRADIID NDWIELFNIN GAIAARAVVS QRVKPGMVMM YHAQEKIVNT PGSEITGARG
     GIHNSVTRIV LKPTHMIGGY AQFSYGFNYY GTIGTNRDEF VVVRKMKRID WLDNEKSAAD
     TSHA
//