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Database: UniProt
Entry: A0A1B3NKS4_9BRAD
LinkDB: A0A1B3NKS4_9BRAD
Original site: A0A1B3NKS4_9BRAD 
ID   A0A1B3NKS4_9BRAD        Unreviewed;       635 AA.
AC   A0A1B3NKS4;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   31-JUL-2019, entry version 17.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN   ECO:0000313|EMBL:AOG05949.1};
GN   ORFNames=BSY19_3652 {ECO:0000313|EMBL:AOG05949.1};
OS   Bosea sp. RAC05.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bosea.
OX   NCBI_TaxID=1842539 {ECO:0000313|EMBL:AOG05949.1, ECO:0000313|Proteomes:UP000094689};
RN   [1] {ECO:0000313|Proteomes:UP000094689}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RAC05 {ECO:0000313|Proteomes:UP000094689};
RA   Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL   Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA
CC       replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC       ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRNR:PIRNR002811};
CC       Note=Binds 1 zinc ion per monomer.
CC       {ECO:0000256|PIRNR:PIRNR002811};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00974}.
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DR   EMBL; CP016464; AOG05949.1; -; Genomic_DNA.
DR   RefSeq; WP_069055955.1; NZ_CP016464.1.
DR   EnsemblBacteria; AOG05949; AOG05949; BSY19_3652.
DR   KEGG; bos:BSY19_3652; -.
DR   PATRIC; fig|1842539.3.peg.3735; -.
DR   KO; K02316; -.
DR   OrthoDB; 1071997at2; -.
DR   Proteomes; UP000094689; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   PIRSF; PIRSF002811; DnaG; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000094689};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000094689};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN      257    339       Toprim. {ECO:0000259|PROSITE:PS50880}.
FT   REGION      428    453       Disordered. {ECO:0000256|SAM:MobiDB-
FT                                lite}.
FT   COILED      590    610       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   635 AA;  69521 MW;  2CB408DAC9052BFA CRC64;
     MKFPPSLLEE IKARLPVSAV VGKRVRLAKA GREWKGLSPF NAEKTPSFFV NDHKASFFDF
     SSGKNGDIFK FVMETEGLSF PEAVEKLAAE AGVTLPKITQ EAQVQEERRK GLHEVVELAA
     RFFEVELMGD RGGQARRYLA SRGLDGEART RFRLGYGPAE RFALRDHLAG KGVSAELMME
     AGLLVHGEEI AVPYDRFRDR IMFPIHDSRG RVVAFGGRAM SAEVSAKYLN SPETPLFHKG
     SLLFNHHNAR KAAHDTGQAI VVEGYVDVIA MALAGFPQTV APLGTALTED QLTLLWRMAD
     EPVICLDGDK AGRKAASRAI DLALPLLEPG KSLSFALLPE GQDPDDLARS GGKAAIADVL
     AEARPLVDML WTREVEAGPL DTPERRAAFE RRLKEPLGQI RDETTRKHYR REMDERLGQL
     FASAAPARAE RREGGYPRGR GQGGGAVRGG RGGFPQVSPW SVGPLKASSQ LTRTKIMARG
     RGEDTREAFI LLALASHPAL ILRCADEIAD LSLDGPAAER FRQALLDAAM EGVLDEEALA
     TRLQQPRVQE AQAQLMAVTG PAERLKLAQA ADPESVFDSI RQAIVLHHRA RTLHSELKAA
     ERALADEATE ANFAWIKDVK ARLETIEGTE AMSED
//
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