ID A0A1B3SKW9_9MOLU Unreviewed; 792 AA.
AC A0A1B3SKW9;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=protein-N(pi)-phosphohistidine--D-fructose phosphotransferase {ECO:0000256|ARBA:ARBA00012799};
DE EC=2.7.1.202 {ECO:0000256|ARBA:ARBA00012799};
GN Name=fruA {ECO:0000313|EMBL:AOG60582.1};
GN ORFNames=SHELI_v1c06310 {ECO:0000313|EMBL:AOG60582.1};
OS Spiroplasma helicoides.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=216938 {ECO:0000313|EMBL:AOG60582.1, ECO:0000313|Proteomes:UP000094378};
RN [1] {ECO:0000313|EMBL:AOG60582.1, ECO:0000313|Proteomes:UP000094378}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TABS-2 {ECO:0000313|EMBL:AOG60582.1,
RC ECO:0000313|Proteomes:UP000094378};
RA Shen W.-Y., Lo W.-S., Lai Y.-C., Kuo C.-H.;
RT "Complete genome sequence of Spiroplasma helicoides TABS-2 (DSM 22551).";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose(out) + N(pros)-phospho-L-histidyl-[protein] = D-
CC fructose 1-phosphate(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:49252, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:29979, ChEBI:CHEBI:37721, ChEBI:CHEBI:58674,
CC ChEBI:CHEBI:64837; EC=2.7.1.202;
CC Evidence={ECO:0000256|ARBA:ARBA00001401};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CP017015; AOG60582.1; -; Genomic_DNA.
DR RefSeq; WP_069116627.1; NZ_CP017015.1.
DR AlphaFoldDB; A0A1B3SKW9; -.
DR STRING; 216938.SHELI_v1c06310; -.
DR KEGG; shj:SHELI_v1c06310; -.
DR PATRIC; fig|216938.3.peg.643; -.
DR OrthoDB; 9782569at2; -.
DR Proteomes; UP000094378; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR GO; GO:0022877; F:protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR CDD; cd00211; PTS_IIA_fru; 1.
DR CDD; cd05569; PTS_IIB_fructose; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR InterPro; IPR004715; PTS_IIA_fruc.
DR InterPro; IPR003353; PTS_IIB_fruc.
DR InterPro; IPR006327; PTS_IIC_fruc.
DR NCBIfam; TIGR00829; FRU; 1.
DR NCBIfam; TIGR00848; fruA; 1.
DR NCBIfam; TIGR01427; PTS_IIC_fructo; 1.
DR PANTHER; PTHR30505; FRUCTOSE-LIKE PERMEASE; 1.
DR PANTHER; PTHR30505:SF0; FRUCTOSE-LIKE PTS SYSTEM EIIBC COMPONENT-RELATED; 1.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF55804; Phoshotransferase/anion transport protein; 1.
DR SUPFAM; SSF52794; PTS system IIB component-like; 1.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000094378};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 299..320
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 455..477
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 497..525
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 587..610
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 650..672
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 692..720
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 5..149
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000259|PROSITE:PS51094"
FT DOMAIN 163..259
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000259|PROSITE:PS51099"
FT DOMAIN 288..720
FT /note="PTS EIIC type-2"
FT /evidence="ECO:0000259|PROSITE:PS51104"
SQ SEQUENCE 792 AA; 85113 MW; FC8818390489E2C5 CRC64;
MELKELFHKK IAFFETDLNS KDEVIDFLTS TLEKEKFIKD AKAFKDAVYK REGEGSTGIG
DGIAIPHVLN PTVNQSAIAF CKLKNKIDWQ SLDGQPADLI FMIMTNGKDG NEHLDALANL
SGYLIKAEVQ EGLRKAKSVD KVKELLSQPK EKREVKGDGG YDVVGITACP TGIAHTYLAA
EKLQEYANQL GMSAKIETQG RRGVEDKLTA KDIENAKVVI LAHDKAINGM SRFNGKKVID
TTTKDAIYNG KDLIEKFDSN PKLKTIENAK DEEDGGSGEL TLSQFKKVKD NLLAGVSRML
PFVVAGGIVL GLGFLLDYIY TAANGMIAVA SLDDFEKYGL GNVITGYTEK NEAVHLTYKA
FTGMAGSIDY AGQTYYSSGS FGTIWQVAHF FSGVGKIGME LMIPILAAYV CYSIVGPQGL
MVGAVAGLMA NGDGMVYGAI GSWSGAWTRF LPKDLSGLSS GFIGALVGAY LGGLVVFGWT
KAMKKFGKGL QGVRDIVFIP VLSLICMAVI MLAINIPLGY VMYGLQQGIT FMAEKNLMAI
VCIIVALMMC VDMGGPINKI AYTLGTLSVG YALVKDSSSP AYEMQTMIMT AALCGGMVPP
LGIAVSTVIF PRQWTQKAKD AAKTNWLMGA CFISEGAIPF MIEDPKRIPV SAIVGGAITG
AVVGIFKITI TAPHGGIFVS PLTNSLLFSG DAIQKAMGVV FFFVAVLVGA VVMGVILGFW
RLADIRNGKL VLSGTNGVKE GLEVKLAKLN AKNNQSGVAK INSKIQKYNE FEKALLIKQE
AHKKNIESKV KK
//
