ID A0A1B6ASP4_9ACTN Unreviewed; 245 AA.
AC A0A1B6ASP4;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 11-DEC-2019, entry version 14.
DE RecName: Full=Urease {ECO:0000256|SAAS:SAAS00312638};
DE EC=3.5.1.5 {ECO:0000256|SAAS:SAAS00312638};
GN ORFNames=SSP35_19_01120 {ECO:0000313|EMBL:GAU70475.1};
OS Streptomyces sp. NBRC 110611.
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=1621259 {ECO:0000313|EMBL:GAU70475.1, ECO:0000313|Proteomes:UP000094479};
RN [1] {ECO:0000313|EMBL:GAU70475.1, ECO:0000313|Proteomes:UP000094479}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 110611 {ECO:0000313|EMBL:GAU70475.1,
RC ECO:0000313|Proteomes:UP000094479};
RA Igarashi Y., Komaki H., Ichikawa N., Hosoyama A., Fujita N., Panbangred W.;
RT "Whole genome shotgun sequence of Streptomyces sp. NBRC 110611.";
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC Evidence={ECO:0000256|SAAS:SAAS01117045};
CC -!- COFACTOR:
CC Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC Evidence={ECO:0000256|SAAS:SAAS00607110};
CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC urea (urease route): step 1/1. {ECO:0000256|SAAS:SAAS00312627}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|SAAS:SAAS00534747}.
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC Urease alpha subunit family. {ECO:0000256|SAAS:SAAS00846840}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAU70475.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BBUY01000019; GAU70475.1; -; Genomic_DNA.
DR EnsemblBacteria; GAU70475; GAU70475; SSP35_19_01120.
DR UniPathway; UPA00258; UER00370.
DR Proteomes; UP000094479; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.30.40.10; -; 1.
DR InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR InterPro; IPR032466; Metal_Hydrolase.
DR InterPro; IPR011612; Urease_alpha_N_dom.
DR InterPro; IPR029754; Urease_Ni-bd.
DR Pfam; PF00449; Urease_alpha; 1.
DR SUPFAM; SSF51338; SSF51338; 1.
DR SUPFAM; SSF51556; SSF51556; 1.
DR PROSITE; PS01120; UREASE_1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|SAAS:SAAS00312633};
KW Hydrolase {ECO:0000256|SAAS:SAAS00321417};
KW Metal-binding {ECO:0000256|SAAS:SAAS00321440};
KW Nickel {ECO:0000256|SAAS:SAAS00312631};
KW Reference proteome {ECO:0000313|Proteomes:UP000094479}.
FT DOMAIN 3..126
FT /note="Urease_alpha"
FT /evidence="ECO:0000259|Pfam:PF00449"
FT REGION 158..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..181
FT /note="Basic"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..245
FT /note="Polyampholyte"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 245 AA; 26369 MW; DC9FBF21D041D6FD CRC64;
MAELTRQAYA GLFGPTAGDR IRLADTDLVV EITEDRSGGP GRAGDEAVFG GGKVIRESMG
QSRVTRAEGA PDTVITGAVV LDHWGIVKAD VGIRDGRITA LGKAGNPDTM DGVHPGLVIG
PETEIIAGNG KILTAGAIDT HVHFICPQQA DEALASGLRR HHDDRRRHRT GRGQQGDHHH
PRRLARRPDA GVDGLPAGQR RTPRQGQHHL PRLDARPAAG RRPRLQDPRG LGRHPRGARR
LPDRL
//
