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Database: UniProt
Entry: A0A1B6B9K7_9FIRM
LinkDB: A0A1B6B9K7_9FIRM
Original site: A0A1B6B9K7_9FIRM 
ID   A0A1B6B9K7_9FIRM        Unreviewed;       803 AA.
AC   A0A1B6B9K7;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   08-MAY-2019, entry version 12.
DE   SubName: Full=Enoyl-CoA hydratase {ECO:0000313|EMBL:GAU76421.1};
DE            EC=4.2.1.17 {ECO:0000313|EMBL:GAU76421.1};
GN   ORFNames=F3D3_1018 {ECO:0000313|EMBL:GAU76421.1};
OS   Fusibacter sp. 3D3.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales;
OC   Clostridiales Family XII. Incertae Sedis; Fusibacter.
OX   NCBI_TaxID=1048380 {ECO:0000313|EMBL:GAU76421.1, ECO:0000313|Proteomes:UP000095197};
RN   [1] {ECO:0000313|EMBL:GAU76421.1, ECO:0000313|Proteomes:UP000095197}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3D3 {ECO:0000313|EMBL:GAU76421.1,
RC   ECO:0000313|Proteomes:UP000095197};
RA   Serrano A.E., Escudero L.V., Encalada O., Tebes C.J., Fernandez S.,
RA   Demergasso C.S.;
RT   "Draft genome sequence of Fusibacter ascotence 3D3 isolated from the
RT   high arsenic level Ascotan salt flat in northern Chile.";
RL   Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+)
CC         + NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57318, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:90726; EC=1.1.1.35;
CC         Evidence={ECO:0000256|SAAS:SAAS01123908};
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:GAU76421.1}.
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DR   EMBL; BDHH01000003; GAU76421.1; -; Genomic_DNA.
DR   EnsemblBacteria; GAU76421; GAU76421; F3D3_1018.
DR   Proteomes; UP000095197; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:InterPro.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1040.10; -; 2.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR032259; HIBYL-CoA-H.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF16113; ECH_2; 1.
DR   SUPFAM; SSF48179; SSF48179; 2.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000095197};
KW   Fatty acid metabolism {ECO:0000256|SAAS:SAAS00324839};
KW   Lipid metabolism {ECO:0000256|SAAS:SAAS00324863};
KW   Lyase {ECO:0000313|EMBL:GAU76421.1};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   NAD {ECO:0000256|SAAS:SAAS00830857};
KW   Reference proteome {ECO:0000313|Proteomes:UP000095197};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM     28     53       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN       28    225       3HCDH_N. {ECO:0000259|Pfam:PF02737}.
FT   DOMAIN      228    327       3HCDH. {ECO:0000259|Pfam:PF00725}.
SQ   SEQUENCE   803 AA;  89464 MW;  B841E7499204F85D CRC64;
     MASILRYRMV YQNLIKEVKR MQYQMNRVAI IGAGVMGASI AALIAGTGTP VILLDILPNE
     LSPEEIEKGL SNSDKVFRDK FAVAGKEKVL NPRNRAIYHK DFGDLITTGN IEDDLSLLKD
     CDWVLEVVLE ELEVKQQLFK KMRPYIGEKT IISSNTSGVS IRKIAEPFDE EFKKRFLGTH
     FFNPPRYMKL FEIIPTAETA PSIVDFMTVY ATKLLGKGVV YAKDTPNFIA NRIGAQANVA
     VMQLAEKYQY SIPKSDMLSG EIIGRPKTAT FKTIDMVGLD ILLHVADNVM NNITSTYEAK
     VYRVPDYVRK LASMGYYGDK VGNGFYKKQK TAQGVVKSVW DCDTESYTEI SKVELDIMKV
     VKEGDTLAEK LNLVVWGDAE EQHFIWDVIK ANLLYSAYCM PEIADRVTEI DNGMKWGFNW
     QLGPFEIWDA LGVSKTVKRM QAEGEAVPEW VIKHLEAGHK TFYEDIDETK SYIVLDRKSD
     TILRSNEDAS LVDLGDGVAC LTFKTKGNTV NRGVIEMINK SVEIVENGDF KGLVIGNQSK
     NFSAGADLAL IGELAEKKAW KALETLIHDF QYANLALKYC HKPVVSAPRG MTLGGGAEMA
     LHAHRQVVTA ETYMGLVELG VGLIPGGGGC KELLARAMLD MDKAPLAERV THVKRIWRNI
     AMAKVSSSGY DAKKLGFVRL GDVFNMNADL QLTDAKNEVI KLSQTAYMGN LKTPINMTGK
     TGFAAIKLEI MQMVDGHFIS AYDGMLAEKV AYILTGGNVL SNTVMNEETI LDLEREIFLS
     LCGEAKTQER IRHMLTRGKP LRN
//
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