ID   A0A1B6YJ61_9RHOB        Unreviewed;       768 AA.
AC   A0A1B6YJ61;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=Penicillin acylase {ECO:0000313|EMBL:OAN68635.1};
GN   ORFNames=A8B83_17560 {ECO:0000313|EMBL:OAN68635.1};
OS   Rhodobacteraceae bacterium EhC02.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae.
OX   NCBI_TaxID=1849170 {ECO:0000313|EMBL:OAN68635.1, ECO:0000313|Proteomes:UP000078223};
RN   [1] {ECO:0000313|EMBL:OAN68635.1, ECO:0000313|Proteomes:UP000078223}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EhC02 {ECO:0000313|EMBL:OAN68635.1,
RC   ECO:0000313|Proteomes:UP000078223};
RA   Lavstsen T., Jespersen J.S.;
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OAN68635.1}.
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DR   EMBL; LXYH01000031; OAN68635.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1B6YJ61; -.
DR   STRING; 1849170.A8B83_17560; -.
DR   OrthoDB; 9760084at2; -.
DR   Proteomes; UP000078223; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000078223};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        242
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         314
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         317
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   768 AA;  82823 MW;  7414E8036A879606 CRC64;
     MTTYHLPGLA APATLQIDRW GLAHIRAEGL HDLFFSQGFN AARDRLWQID LWRKRGLGLL
     AADFGPGYLM QDRAARLFLY RGDMAPEWAA YGEDAQAICT AFAAGINACV DLVLDGRMAL
     PAEFAALGTQ PAHWAAEDVV RIRTHCLTRN AASELARAQV LALADPDTDL LRVPLNPPVD
     ASEWAVNAGH DLPAHALAVF ELATAPVTFA PDRLAAGLDQ AARWSRVDPR KTVVQAPSME
     GSNNWVVSGA RTASGRPIMA SDPHRAHAAP SLRYMVHLTA PGIDIIGAGE PSSPGIMAGH
     NGHAAFSLTI FCADQEDVMV YDTDPDAPAR YRYADGWEEM QTVTEVFAVR GHPDQRLDLR
     FTRHGPVVWE EPGRALALRT VFTEPGTAPY MASLKSMRAT TWEGFKDAVD TWGAPSVNLV
     YADVAGDFGW QAAGYVPRRN GWRGLVPVAG DGRFEWQGFM TASTLPRLDN PAQGFVHSAN
     EMNLPAAAQA GPAIGHEWFQ DQRAARIAAA LGARDDHDLA TSTALQTDTG SPFAARLIAL
     LPQDAPGRDL LALWDGRSDA GSAPALLYEV WLSSHLRPAL LSRVAPDPGL RRFLVPGNVP
     VVTELLEGRH PRLAARAGLD APDARETLLR ETLAAALADV TARFGADPLQ WRWGDLHHGW
     FDHALTPLGT SFDLGPWPKG GGNTSIMLAH HEASDYRVRI GASVRMVIDL GDWDASVWIN
     APGQSGMQDS PHLADLAPLW AAGEYVPLAY SAAAVEAVTV ETITLLTG
//