ID A0A1B6Z9J5_9SPHN Unreviewed; 513 AA.
AC A0A1B6Z9J5;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN ORFNames=A8B75_12770 {ECO:0000313|EMBL:OAO02024.1};
OS Sphingomonadales bacterium EhC05.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales.
OX NCBI_TaxID=1849171 {ECO:0000313|EMBL:OAO02024.1, ECO:0000313|Proteomes:UP000078455};
RN [1] {ECO:0000313|Proteomes:UP000078455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EhC05 {ECO:0000313|Proteomes:UP000078455};
RA Rosana A., Orata F., Boucher Y., Case R.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAO02024.1}.
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DR EMBL; LXYP01000020; OAO02024.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1B6Z9J5; -.
DR STRING; 1849171.A8B75_12770; -.
DR OrthoDB; 7440981at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000078455; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000078455};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..275
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 316..466
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
FT REGION 477..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 477..492
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 513 AA; 56404 MW; 4470C6411A581137 CRC64;
MKQLSALDAM FAYTETANTP MHIGQLLIYD PSTAPGGAVG FKDILRYIEG RLDGARIFRQ
KLVRVPLDLD HPYWVDDANF DIEFHVRHIA LPKPGDWRQL CIQAARIYAR PMDMNKPLWE
YTVIEGLDNV EGVPKGSFAM LHKMHHAAID GQSGLQMTLA LHDLDAKMKP RKFDMAFEPE
SDPNPIGLLA KAQFNNIANP VRGLQNLQKL IPMPKRLLDV RREFVQRDDA KTSIPKTRFN
KKLSPHRVFD GREFALSDIK KMREAFPGAT VNDVMIATVS GAMRGYLKAK DDLPDTTLKI
GAPVSVRSDD DKDSAGNQVT MMQVGVGTHL EDAGERLQFI MAETQRSKAM TQAMGAKTLM
ELSGAMPAGL TAAGTKMMVR AGLVERLNPA VNSVVTNVPG PMVPMYFAGA ELVKSFGMGI
LAEGQGLFHV VTSYNGNVIL TFLADRNIMP DPEFYATCIS RSFADLMAAA TKAKTAATRR
MKTKKAKTTK APKKRVSASA ATAGGVRRKA VKK
//
